Parameter Overview
| Reactions | kcat [M/s or 1/s] | σcat [M/s or 1/s] | Kms [M] | σKms [M] | Kmp [M] | σKmp [M] | Keq | σKeq |
|---|---|---|---|---|---|---|---|---|
| ERK Activation1 | 4.9198E+00 | 5.1143E-01 | 4.7600E-07 | 6.7416E-08 | 4.9980E-07 | 1.0112E-07 | 1.7700E+02 | 1.0500E+01 |
| ERK Activation2 | 4.9198E+00 | 5.1143E-01 | 4.6600E-08 | 6.6000E-09 | 4.8930E-08 | 9.9000E-09 | 1.7700E+02 | 1.0500E+01 |
| ERK Inactivation1 | 7.0350E-01 | 2.1510E-02 | 1.5020E-06 | 1.3348E-07 | 1.5771E-06 | 2.0022E-07 | 7.5000E+02 | 1.1753E+02 |
| ERK Inactivation2 | 7.0350E-01 | 2.1510E-02 | 1.5020E-06 | 2.0022E-07 | 1.5771E-06 | 3.0033E-07 | 7.5000E+02 | 1.1753E+02 |
| MEK Activation (B-Raf) | 2.5083E-01 | 2.3908E-02 | 2.7500E-07 | 1.0011E-06 | 2.8875E-07 | 1.5016E-06 | 1.7700E+02 | 1.0500E+01 |
| MEK Activation (Raf1) | 2.5083E-01 | 3.5862E-02 | 8.0000E-07 | 4.2788E-07 | 8.4000E-07 | 6.4182E-07 | 1.7700E+02 | 1.0500E+01 |
| MEK-Inactivation | 6.3251E+02 | 6.5753E+01 | 3.8307E-03 | 1.3976E-04 | 4.0222E-03 | 2.0964E-04 | 7.5000E+02 | 1.1753E+02 |
| Raf1 Activation | 1.7658E+01 | 1.8356E+00 | 5.4228E+03 | 2.9004E+03 | 5.6939E+03 | 4.3505E+03 | 1.7700E+02 | 1.0500E+01 |
| Raf1 Inactivation | 1.3120E+04 | 1.3639E+03 | 3.8307E-03 | 1.3976E-04 | 4.0222E-03 | 2.0964E-04 | 7.5000E+02 | 1.1753E+02 |
| Raf1-Removal | 1.9248E+00 | 9.6200E-01 | 4.2700E-05 | 1.3476E-05 | 4.4835E-05 | 2.0214E-05 | 1.7700E+02 | 1.0500E+01 |
| Ras-Activation | 8.8992E-02 | 9.2511E-03 | 5.4228E+03 | 2.9004E+03 | 5.6939E+03 | 4.3505E+03 | 3.0700E+00 | 4.8110E-01 |
| Ras-Inactivation (GAP) | 7.7330E+00 | 2.5500E+00 | 8.5800E-06 | 9.4050E-06 | 9.0090E-06 | 1.4108E-05 | 5.8000E+05 | 9.0892E+04 |
| Sos-Removal alternative value | 3.8911E-03 | 4.0450E-04 | ||||||
| Sos-Removal(ERKP) | 3.1600E+01 | 1.1000E-01 | 6.5600E-04 | 6.2000E-05 | 6.8880E-04 | 9.3000E-05 | 1.7700E+02 | 1.0500E+01 |
| Sos-Removal(ERKPP) | 6.9900E+00 | 1.0900E-01 | 2.8000E-03 | 6.9100E-04 | 2.9400E-03 | 1.0365E-03 | 1.7700E+02 | 1.0500E+01 |
| B-Raf-Activation (Rap1) | 1.7211E+01 | 1.7892E+00 | 9.6653E+03 | 5.1695E+03 | 1.0149E+04 | 7.7542E+03 | 1.7700E+02 | 1.0500E+01 |
| B-Raf-Activation (Ras) | 5.8585E+00 | 6.0902E-01 | 2.4322E+03 | 1.3009E+03 | 2.5538E+03 | 1.9513E+03 | 1.7700E+02 | 1.0500E+01 |
| B-Raf-Inactivation | 7.5932E+00 | 7.8935E-01 | 3.8307E-03 | 1.3976E-04 | 4.0222E-03 | 2.0964E-04 | 7.5000E+02 | 1.1753E+02 |
| C3G-Activation | 2.0549E+01 | 1.3256E+02 | 1.3480E-07 | 2.4020E-07 | 1.4154E-07 | 3.6030E-07 | 7.4104E+01 | 3.3443E+01 |
| C3G-Inactivation | 2.1099E+03 | 2.1933E+02 | 3.8307E-03 | 1.3976E-04 | 4.0222E-03 | 2.0964E-04 | 7.4104E+01 | 3.4443E+01 |
| Rap1-Activation (C3G) | 7.2000E-04 | 6.0000E-05 | 2.0200E-05 | 2.5050E-06 | 2.1210E-05 | 3.7575E-06 | 3.0700E+00 | 4.8110E-01 |
| Rap1-Activation (EPAC) | 7.2000E-04 | 1.9000E-04 | 2.0200E-05 | 1.6700E-06 | 2.1210E-05 | 2.5050E-06 | 3.0700E+00 | 4.8110E-01 |
| Rap1-Inactivation (GAP) | 7.7330E+00 | 2.8500E-04 | 8.5800E-06 | 1.0973E-05 | 9.0090E-06 | 1.6459E-05 | 5.8000E+05 | 9.0892E+04 |
| EPAC-Activation (Cilostamide) | 9.3451E+00 | - | 9.6999E+03 | - | 1.0185E+04 | - | 1.0000E+10 | - |
| EPAC-Activation (EPACA) | 4.0753E+00 | 4.2365E-01 | 1.7400E-05 | 1.4081E-05 | 1.8270E-05 | 2.1122E-05 | 2.0000E+00 | 9.0000E-01 |
| EPAC-Inactivation | 8.7276E+02 | 9.0727E+01 | 8.3204E+03 | 6.7334E+03 | 8.7364E+03 | 1.0100E+04 | 2.0000E+00 | 9.0000E-01 |
| PKA-Activation (Cilostamide) | 1.6605E+00 | - | 3.1023E+03 | - | 3.2574E+03 | - | 1.0000E+10 | - |
| PKA-Activation (PKAA) | 1.2010E+00 | 1.2485E-01 | 1.7400E-05 | 2.1122E-05 | 1.8270E-05 | 3.1683E-05 | 2.0000E+00 | 1.3500E+00 |
| PKA-Inactivation | 1.3519E+04 | 1.4054E+03 | 9.6812E+03 | 1.1752E+04 | 1.0165E+04 | 1.7628E+04 | 2.0000E+00 | 1.3500E+00 |
| kass [1/Ms] | σkass [1/µMs] | kdiss [1/s] | σkdiss [1/s] | |||||
| Binding EGF to EGFR | 1.0700E+06 | 2.4000E+05 | 2.3800E-03 | 5.9100E-04 | ||||
| Binding Sos to EGFR | 1.4700E+08 | 6.1310E+07 | 1.4880E-01 | 3.9650E-02 |
Parameter of Model Version 2 (modified structure)
| Reactions | kcat [1/µMs or 1/s] | σcat [1/µMs or 1/s] | Kms [µM] | σKms [µM] | Kmp [µM] | σKmp [µM] | Keq | σKeq |
|---|---|---|---|---|---|---|---|---|
| ERK Activation1 | 6.4843E-07 | 1.1383E-07 | 4.7600E-01 | 6.7416E-02 | 4.5220E-01 | 1.0112E-01 | 1.7700E+02 | 1.0500E+01 |
| ERK Activation2 | 6.4843E-07 | 1.1383E-07 | 4.6600E-02 | 6.6000E-03 | 4.4270E-02 | 9.9000E-03 | 1.7700E+02 | 1.0500E+01 |
| ERK Inactivation1 | 7.0500E-01 | 1.8838E-02 | 1.5000E+00 | 1.2438E-02 | 1.4250E+00 | 1.8656E-02 | 1.7700E+02 | 1.0500E+01 |
| ERK Inactivation2 | 1.0550E+00 | 5.9101E-02 | 4.6700E+00 | 5.6607E-01 | 4.4365E+00 | 8.4911E-01 | 1.7700E+02 | 1.0500E+01 |
| MEK Activation (B-Raf) | 2.2856E+02 | 1.4773E+02 | 2.7500E-01 | 1.0011E+00 | 2.6125E-01 | 1.5016E+00 | 1.7700E+02 | 1.0500E+01 |
| MEK Activation (Raf1) | 2.2856E+02 | 1.4773E+02 | 8.0000E-01 | 3.7626E-01 | 7.6000E-01 | 5.6439E-01 | 1.7700E+02 | 1.0500E+01 |
| MEK-Inactivation | 1.1303E+02 | 3.0888E+02 | 3.8307E+03 | 1.3976E+02 | 3.6392E+03 | 2.0964E+02 | 1.7700E+02 | 1.0500E+01 |
| Raf1 Activation | 1.7658E-05 | 3.0997E-06 | 5.6713E+09 | 2.6674E+09 | 5.3878E+09 | 4.0010E+09 | 1.7700E+02 | 1.0500E+01 |
| Raf1 Inactivation | 1.1303E+02 | 3.0888E+02 | 3.8307E+03 | 1.3976E+02 | 3.6392E+03 | 2.0964E+02 | 1.7700E+02 | 1.0500E+01 |
| Raf1-Removal | 2.0000E+05 | 1.0462E+05 | 1.6250E+01 | 6.1500E+00 | 1.5438E+01 | 9.2250E+00 | 1.7700E+02 | 1.0500E+01 |
| Ras-Inactivation (GAP) | 1.4500E+00 | 2.6250E-01 | 8.5800E+00 | 9.4050E+00 | 8.1510E+00 | 1.4108E+01 | 3.0700E+00 | 4.8110E-01 |
| Sos-Removal(ERKPP) | 1.7560E+01 | 6.1111E-01 | 6.5600E+02 | 6.2000E+01 | 6.2320E+02 | 9.3000E+01 | 1.7700E+02 | 1.0500E+01 |
| Sos-Removal(ERKP) | 3.8800E+00 | 6.0556E-01 | 2.8000E+03 | 6.9100E+02 | 2.6600E+03 | 1.0365E+03 | 1.7700E+02 | 1.0500E+01 |
| B-Raf-Activation (Rap1) | 1.7211E-05 | 3.0213E-06 | 9.6653E+09 | 4.5458E+09 | 9.1820E+09 | 6.8187E+09 | 1.7700E+02 | 1.0500E+01 |
| B-Raf-Activation (Ras) | 5.8585E-06 | 1.0284E-06 | 2.4322E+09 | 1.1439E+09 | 2.3106E+09 | 1.7159E+09 | 1.7700E+02 | 1.0500E+01 |
| B-Raf-Inactivation | 1.1303E+02 | 3.0888E+02 | 3.8307E+03 | 1.3976E+02 | 3.6392E+03 | 2.0964E+02 | 1.7700E+02 | 1.0500E+01 |
| C3G-Activation | 2.0549E+01 | 1.3256E+02 | 1.3480E-01 | 2.4020E-01 | 1.2806E-01 | 3.6030E-01 | 7.4104E+01 | 3.3443E+01 |
| C3G-Inactivation | 1.1303E+02 | 3.0888E+02 | 3.8307E+03 | 1.3976E+02 | 3.6392E+03 | 2.0964E+02 | 7.4104E+01 | 3.4443E+01 |
| Rap1-Activation (C3G) | 7.2000E-03 | 6.0000E-05 | 2.0200E+01 | 2.5050E+00 | 1.9190E+01 | 3.7575E+00 | 3.0700E+00 | 4.8110E-01 |
| Rap1-Activation (EPAC) | 7.2000E-03 | 4.0000E-06 | 2.0200E+01 | 1.6700E+00 | 1.9190E+01 | 2.5050E+00 | 3.0700E+00 | 4.8110E-01 |
| Rap1-Inactivation (GAP) | 1.4500E+00 | 3.0625E-01 | 8.5800E+00 | 1.0973E+01 | 8.1510E+00 | 1.6459E+01 | 3.0700E+00 | 4.8110E-01 |
| EPAC-Activation (Cilostamide) | 9.3451E-06 | - | 9.6999E+09 | - | 9.2149E+09 | - | 1.0000E+10 | - |
| EPAC-Inactivation | 8.7276E+02 | 1.5320E+02 | 8.3204E+03 | 6.7334E+03 | 7.9043E+03 | 1.0100E+04 | 2.0000E+00 | 9.0000E-01 |
| PKA-Activation (Cilostamide) | 1.6605E-06 | - | 3.1023E+09 | - | 2.9472E+09 | - | 1.0000E+10 | - |
| PKA-Inactivation | 1.3519E+04 | 2.3731E+03 | 9.6812E+09 | 1.1752E+10 | 9.1971E+09 | 1.7628E+10 | 2.0000E+00 | 1.3500E+00 |
| kass [1/µMs] | σkass [1/µMs] | kdiss [1/s] | σkdiss [1/s] | |||||
| Binding EGF to EGFR | 1.0700E+00 | 2.4000E-01 | 2.3800E-03 | 5.9100E-04 | ||||
| Binding Sos to EGFR | 1.4700E+02 | 6.1310E+01 | 1.4880E-01 | 3.9650E-02 | ||||
| Activation of Ras (RasGDP to Sos) | 1.1222E+00 | 3.5987E-01 | 6.7333E-04 | 1.7331E-04 | ||||
| Activation of Ras (Dissociation of GDP) | 2.4000E+00 | 7.6965E-01 | 2.0640E+01 | 5.3126E+00 | ||||
| Activation of Ras (Association of GTP) | 2.4000E+00 | 7.6965E-01 | 2.0160E+01 | 5.1890E+00 | ||||
| Activation of Ras (Unbinding of RasGTP) | 3.3667E+00 | 1.0796E+00 | 6.7333E-04 | 1.7331E-04 | ||||
| C3G Binding | 1.4700E+02 | 6.1310E+01 | 1.4880E-01 | 3.9650E-02 | ||||
| PKA Binding | 1.5000E+01 | 8.4000E+00 | 2.1500E+01 | 1.5000E+00 | ||||
| EPAC Binding | 1.5000E+01 | 5.6000E+00 | 2.1500E+01 | 1.0000E+00 |
