EGF-Binding

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Binding Reaction

$EGF + unboundEGFR \rightleftharpoons activeEGFR$

Kinetic Equation

$v_{EGF-binding} = k_{EGF-binding} \cdot [EGF][unboundEGFR]-k_{EGF-release} \cdot [boundEGFR]$

Final Parameter

$k_{EGF-binding}=1.07\;\pm\;0.24\ \frac{1}{\mu Ms}$
$k_{EGF-release}= 2.38 \cdot 10^{-3}\;\pm\;5.91 \cdot 10^{-4}\ \frac{1}{s}$

Parameters

Parameter Calculation	Notes	References
$k_{EGF-binding}=6.3 \cdot 10^7\;\pm\;1.9 \cdot 10^7\ \frac{1}{minM}$ $= 1.05\;\pm\;0.32\ \frac{1}{\mu Ms}$ $k_{EGF-release}= 0.16\;\pm\;0.05\ \frac{1}{min}$ $= 2.6 \cdot 10^{-3}\;\pm\;8.33 \cdot 10^{-4}\ \frac{1}{s}$	Data obtained from at least 3 different experiments; at pH 7.4 and at 37 °C; used for the calculation are the hEGF data	French et al.(1995)
$k_{EGF-binding} = (0.47 \cdot 6.2 * 10^5\; \frac{1}{Ms}$ $+ 0.24 \cdot 3.3 \cdot 10^6\ \frac{1}{Ms} + 0.29 \cdot 3.2 \cdot 10^4\ \frac{1}{Ms})$ $\;\pm\; (0.47 \cdot 0.3 \cdot 10^5\ \frac{1}{Ms} + 0.24 \cdot 0.6 \cdot 10^6\ \frac{1}{Ms}$ $+ 0.29 \cdot 0.4 \cdot 10^4\ \frac{1}{Ms}) = 1.09 \;\pm\; 0.159\ \frac{1}{\mu Ms}$ $k_{EGF-release}$ is calculated analog to $k_{EGF-release} = 2.15 \cdot 10^{-3} \;\pm\; 3.5 \cdot 10^{-4}\ \frac{1}{s}$	Method used: mixture of unlabeled and labeled I-EGF at different concentrations is added to HeLa cells. Radioactivity is measured. Temperature 0 °C The weighted average depending on the amount of the different receptor types is calculated.	Berkers et al. (1991)