Transformation of AA to PC
Contents
Reaction
Catalysed by LAT
Chemical equation
Rate equation
LAT Parameters
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
0.7219 | mM | Human Liver | In vitro
Expression: Human Liver Cell lines (LPCAT3) Substrate: 1-palmitoyl-lysophosphatidylcholine Temperature:Room Temperature pH:unknown |
[1] |
0.7156 | mM | Human Liver | In vitro
Expression: Human Liver Cell lines (LPCAT3) Substrate: Arachidonoyl-CoA Temperature:Room Temperature pH:unknown |
[1] |
0.4129 | mM | Human Liver | In vitro
Expression: Human Liver Cell lines (LPCAT3) Substrate: Palmitoyl-CoA Temperature:Room Temperature pH:unknown |
[1] |
0.558 | mM | Human | In vitro
Expression: Human Embyonic Kidney Cells(HEK293) (LPCAT3) Substrate: Arachidonyl-CoA Temperature: 37 pH:unknown |
[1] |
2.45 | mM | Human | In vitro
Expression: Human Embyonic Kidney Cells(HEK293) (LPCAT3) Substrate: Arachidonyl-CoA Temperature: 37 pH:unknown |
[1] |
0.005 | mM | Human | In vitro
Expression: Yeast (Saccharomyces cerevisiae) Substrate: Arachidonyl-CoA Temperature: 30 pH:7.4 |
[2] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
11.67 | min-1 | Human Neutrophil | In vitro
Expression: Yeast Substrate: Arachidonyl-CoA Temperature: 37 pH:7.5 |
[3] |
0.238 ± 0.004 | min-1 | Recombinant Bacteria | In vitro
Expression: E. Coli Substrate: Capryl-ACP Temperature: unknown pH:unknown |
[4] |
1.483 ± 0.099 | min-1 | Recombinant Bacteria | In vitro
Expression: E. Coli Substrate: Lauryl-ACP Temperature: unknown pH:unknown |
[4] |
0.346± 0.013 | min-1 | Recombinant Bacteria | In vitro
Expression: E. Coli Substrate: Myristyl-ACP Temperature: unknown pH:unknown |
[4] |
0.057 ± 0.009 | min-1 | Recombinant Bacteria | In vitro
Expression: E. Coli Substrate: Palmityl-ACP Temperature: unknown pH:unknown |
[4] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
215 | ppm | Human | Expression Vector: Lung
Enzyme: LAT pH: 7.5 Temperature: 37 °C |
[5] |
16 | Human | Expression Vector: Esophagus
Enzyme: LAT pH: 7.5 Temperature: 37 °C |
[6] | |
6.44 | Human | Expression Vector: Oral Cavity
Enzyme: LAT pH: 7.5 Temperature: 37 °C |
[6] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
325.0658 | kcal/mol | Not stated | Estimated
Enzyme: LAT Substrate: acyl CoA Product: Phosphatidylcholine pH: 7.3 ionic strength: 0.25 |
[7] |
(-331.11646) | kcal/mol | Not stated | Estimated
Enzyme: FACL4 Substrate: FA Product: FA-acyl CoA pH: 7.3 ionic strength: 0.25 |
[7] |
(-8.398743) | kcal/mol | Not stated | Estimated
Enzyme: Transacylase Substrate: Product: pH: 7.3 ionic strength: 0.25 |
[7] |
References
- ↑ 1.0 1.1 1.2 1.3 1.4 Zhao Y. “Identification and Characterization of a Major Liver Lysophosphatidylcholine Acyltransferase” J Biochem. 283, 8258-8265.
- ↑ Kazachkov M. “Substrate Preferences of a Lysophosphatidylcholine Acyltransferase Highlight Its Role in Phospholipid Remodeling” Lipids (2008) 43: 895. doi:10.1007/s11745-008-3233-y
- ↑ S. “Lysophospholipid Acyltransferases and Arachidonate Recycling in Human Neutrophils” J Biol Chem. 2008 Oct 31; 283(44): 30235–30245.
- ↑ 4.0 4.1 4.2 4.3 D. Dovala“Structure-guided enzymology of the lipid A acyltransferase LpxM reveals a dual activity mechanism.” Proc Natl Acad Sci U S A. 2016 Oct 11;113(41):E6064-E6071. Epub 2016 Sep 28.
- ↑ M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
- ↑ 6.0 6.1 M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587
- ↑ 7.0 7.1 7.2 Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471