Transformation of AA to PGH2

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Reaction

R2 AA-PGH2.jpg

Chemical equation

AA \rightleftharpoons PGH2

Rate equation

COX Enzyme Parameters

Gibbs Free Energy Change
Value Units Species Notes Reference
15.0 kcal/mol Sheep Calculated

Enzyme: COX (Unspecific) Substrate: Arachidonate pH: 7.2 Temperature: 30 °C

[1]

COX-1 Enzyme Parameters

COX-1 Michaelis-Menten Constants (When AA is the substrate)
Value Units Species Notes Reference
0.0088 ± 0.0022 mM Human Expression Vector: Human

Enzyme: Cyclooxygenase-1 pH: 7.6 - 8.4 Temperature: 37 °C

[2]
COX-1 Abundance
Value Units Species Notes Reference
1176 ppm Human Expression Vector: Urinary Bladder

Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C

[3]
849 ppm Human Expression Vector: Platlet

Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C

[3]
208 ppm Human Expression Vector: Stomach

Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C

[4]
130 ppm Human Expression Vector: Esophagus

Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C

[4]
70.8 ppm Human Expression Vector: Oral Cavity

Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C

[4]
19.1 ppm Human Expression Vector: Lung

Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C

[3]

COX-2 Enzyme Parameters

COX-2 Michaelis-Menten Constants (When AA is the substrate)
Value Units Species Notes Reference
1.62E-02 ± 0.22E-02 mM Human Expression Vector: Embryonic kidney cells

Enzyme: Cyclooxygenase-2 pH: Not specified Temperature: Not specified

[5]
5.14E-03 ± 2.90E-04 mM Mouse Expression Vector: Baculovirus (Insect Cell)

Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.

[6]
2.1E-03 ± 4.00E-04 mM Human Expression Vector: E. Coli

Enzyme: Wild Type Cyclooxygenase-2 Enzyme pH: 8.5 Temperature:30 °C

[7]
2.1E-03 ± 4.00E-04 mM Human Expression Vector: Baculuvirus

Enzyme: Wild Type Cycloxygenase-2 pH: 7.2 Temperature: 30 °C 100 uM arachidonate substrate,

[8]
Enzyme Turnover Numbers (When AA is the substrate)
Value Units Species Notes Reference
1620 ± 24 per minute Mouse Expression Vector: Baculovirus (Insect Cell)

Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.

[6]


COX-2 Abundance
Value Units Species Notes Reference
13.7 ppm Human Expression Vector: Platlet

Enzyme: Cyclooxygenase-2 (PGTS2) pH: 7.5 Temperature: 37 °C

[3]
4.11 ppm Human Expression Vector: Stomach

Enzyme: Cyclooxygenase-2 (PGTS2) pH: 7.5 Temperature: 37 °C

[4]
1.49 ppm Human Expression Vector: Oral Cavity

Enzyme: Cyclooxygenase-2 (PGTS2) pH: 7.5 Temperature: 37 °C

[4]


COX Enzyme Parameters with other substrates

Michaelis-Menten Constants (When EPA is the substrate)
Value Units Species Notes Reference
9.45E-03 ± 7.30E-04 mM Mouse Expression Vector: Baculovirus (Insect Cell)

Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.

[6]
Enzyme Turnover Numbers (When EPA is the substrate)
Value Units Species Notes Reference
522 ± 12.6 per minute Mouse Expression Vector: Baculovirus (Insect Cell)

Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.

[6]
Michaelis-Menten Constants (When DHA is the substrate)
Value Units Species Notes Reference
3.68E-02 ± 4.25E-03 mM Mouse Expression Vector: Baculovirus (Insect Cell)

Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.

[6]
Enzyme Turnover Numbers (When DHA is the substrate)
Value Units Species Notes Reference
207 ± 9 per minute Mouse Expression Vector: Baculovirus (Insect Cell)

Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.

[6]

References

  1. ↑ [http://www.jbc.org/content/269/8/5527 R J Kulmacz et al 2014, "Interaction between peroxidase and cyclooxygenase activities in prostaglandin-endoperoxide synthase. Interpretation of reaction kinetics" 1994 The Journal of Biological Chemistry 269, 5527-5536.]
  2. ↑ Y. Noreen Development of a Radiochemical Cyclooxygenase-1 and -2 in Vitro Assay for Identification of Natural Products as Inhibitors of Prostaglandin BiosynthesisJ. Nat. Prod., 1998, 61 (1), pp 2–7)
  3. ↑ 3.0 3.1 3.2 3.3 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
  4. ↑ 4.0 4.1 4.2 4.3 4.4 [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587]
  5. ↑ S.F. Kim Inducible nitric oxide synthase binds, S-nitrosylates, and activates cyclooxygenase-2. Science 2005,310(5756):1966-70)
  6. ↑ 6.0 6.1 6.2 6.3 6.4 6.5 [www.ncbi.nlm.nih.gov/pubmed/20463020 Vecchio A. J. "Structural basis of fatty acid substrate binding to cyclooxygenase-2." J. Biol. Chem. 285 22152-63 (2010)]
  7. ↑ [http://pubs.acs.org/doi/pdf/10.1021/bi035717o Rogge C. "Identification of Tyr504 as an Alternative Tyrosyl Radical Site in Human Prostaglandin H Synthase-2" Biochemistry 2004, 43, 1560-1568]
  8. ↑ [http://www.jbc.org/content/279/6/4084.full.pdf Bambai B. "Role of Asn-382 and Thr-383 in Activation and Inactivation of Human Prostaglandin H Synthase Cyclooxygenase Catalysis" February 6, 2004 The Journal of Biological Chemistry, 279, 4084-4092.]

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