Transformation of AA to PGH2

Reaction

AA \rightleftharpoons PGH2

COX-1 Michaelis-Menten Constants (When AA is the substrate)
Value	Units	Species	Notes	Reference
0.0088 ± 0.0022	$mM$	Human	Expression Vector: Human Enzyme: Cyclooxygenase-1 pH: 7.6 - 8.4 Temperature: 37 °C	^[1]

COX-1 Abundance
Value	Units	Species	Notes	Reference
1176	$ppm$	Human	Expression Vector: Urinary Bladder Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C	^[2]
849	$ppm$	Human	Expression Vector: Platlet Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C	^[2]
208	$ppm$	Human	Expression Vector: Stomach Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C	^[3]
130	$ppm$	Human	Expression Vector: Esophagus Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C	^[3]
70.8	$ppm$	Human	Expression Vector: Oral Cavity Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C	^[3]
19.1	$ppm$	Human	Expression Vector: Lung Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C	^[2]

COX-2 Michaelis-Menten Constants (When AA is the substrate)
Value	Units	Species	Notes	Reference
1.62E-02 ± 0.22E-02	$mM$	Human	Expression Vector: Embryonic kidney cells Enzyme: Cyclooxygenase-2 pH: Not specified Temperature: Not specified	^[4]
5.14E-03 ± 2.90E-04	mM	Mouse	Expression Vector: Baculovirus (Insect Cell) Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.	^[5]
2.1E-03 ± 4.00E-04	mM	Human	Expression Vector: E. Coli Enzyme: Wild Type Cyclooxygenase-2 Enzyme pH: 8.5 Temperature:30 °C	^[6]
2.1E-03 ± 4.00E-04	mM	Human	Expression Vector: Baculuvirus Enzyme: Wild Type Cycloxygenase-2 pH: 7.2 Temperature: 30 °C 100 uM arachidonate substrate,	^[7]

Enzyme Turnover Numbers (When AA is the substrate)
Value	Units	Species	Notes	Reference
1620 ± 24	per minute	Mouse	Expression Vector: Baculovirus (Insect Cell) Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.	^[5]

COX-2 Abundance
Value	Units	Species	Notes	Reference
13.7	$ppm$	Human	Expression Vector: Platlet Enzyme: Cyclooxygenase-2 (PGTS2) pH: 7.5 Temperature: 37 °C	^[2]
4.11	$ppm$	Human	Expression Vector: Stomach Enzyme: Cyclooxygenase-2 (PGTS2) pH: 7.5 Temperature: 37 °C	^[3]
1.49	$ppm$	Human	Expression Vector: Oral Cavity Enzyme: Cyclooxygenase-2 (PGTS2) pH: 7.5 Temperature: 37 °C	^[3]

Michaelis-Menten Constants (When EPA is the substrate)
Value	Units	Species	Notes	Reference
9.45E-03 ± 7.30E-04	mM	Mouse	Expression Vector: Baculovirus (Insect Cell) Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.	^[5]

Enzyme Turnover Numbers (When EPA is the substrate)
Value	Units	Species	Notes	Reference
522 ± 12.6	per minute	Mouse	Expression Vector: Baculovirus (Insect Cell) Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.	^[5]

Michaelis-Menten Constants (When DHA is the substrate)
Value	Units	Species	Notes	Reference
3.68E-02 ± 4.25E-03	mM	Mouse	Expression Vector: Baculovirus (Insect Cell) Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.	^[5]

Enzyme Turnover Numbers (When DHA is the substrate)
Value	Units	Species	Notes	Reference
207 ± 9	per minute	Mouse	Expression Vector: Baculovirus (Insect Cell) Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.	^[5]

Enzyme Concentration
Value	Units	Species	Notes	Reference
1.45e-02	mM	Mouse	Expression Vector: Baculovirus (Insect Cell) Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.	^[5]

↑ Y. Noreen Development of a Radiochemical Cyclooxygenase-1 and -2 in Vitro Assay for Identification of Natural Products as Inhibitors of Prostaglandin BiosynthesisJ. Nat. Prod., 1998, 61 (1), pp 2–7)
↑ ^2.0 ^2.1 ^2.2 ^2.3 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587]
↑ S.F. Kim Inducible nitric oxide synthase binds, S-nitrosylates, and activates cyclooxygenase-2. Science 2005,310(5756):1966-70)
↑ ^5.0 ^5.1 ^5.2 ^5.3 ^5.4 ^5.5 ^5.6 [www.ncbi.nlm.nih.gov/pubmed/20463020 Vecchio A. J. "Structural basis of fatty acid substrate binding to cyclooxygenase-2." J. Biol. Chem. 285 22152-63 (2010)]
↑ [http://pubs.acs.org/doi/pdf/10.1021/bi035717o Rogge C. "Identification of Tyr504 as an Alternative Tyrosyl Radical Site in Human Prostaglandin H Synthase-2" Biochemistry 2004, 43, 1560-1568]
↑ [http://www.jbc.org/content/279/6/4084.full.pdf Bambai B. "Role of Asn-382 and Thr-383 in Activation and Inactivation of Human Prostaglandin H Synthase Cyclooxygenase Catalysis" February 6, 2004 The Journal of Biological Chemistry, 279, 4084-4092.]