Transformation of AA to PGH2
Contents
Reaction
Chemical equation
Rate equation
COX-1 Enzyme Parameters
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
0.0088 ± 0.0022 | Human | Expression Vector: Human
Enzyme: Cyclooxygenase-1 pH: 7.6 - 8.4 Temperature: 37 °C |
[1] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
1176 | Human | Expression Vector: Urinary Bladder
Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C |
[2] |
COX-2 Enzyme Parameters
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
1.62E-02 ± 0.22E-02 | Human | Expression Vector: Embryonic kidney cells
Enzyme: Cyclooxygenase-2 pH: Not specified Temperature: Not specified |
[3] | |
5.14E-03 ± 2.90E-04 | mM | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[4] |
2.1E-03 ± 4.00E-04 | mM | Human | Expression Vector: E. Coli
Enzyme: Wild Type Cyclooxygenase-2 Enzyme pH: 8.5 Temperature:30 °C |
[5] |
2.1E-03 ± 4.00E-04 | mM | Human | Expression Vector: Baculuvirus
Enzyme: Wild Type Cycloxygenase-2 pH: 7.2 Temperature: 30 °C 100 uM arachidonate substrate, |
[6] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
1620 ± 24 | per minute | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[4] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
9.45E-03 ± 7.30E-04 | mM | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[4] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
522 ± 12.6 | per minute | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[4] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
3.68E-02 ± 4.25E-03 | mM | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[4] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
207 ± 9 | per minute | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[4] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
1.45e-02 | mM | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[4] |
References
- ↑ Y. Noreen Development of a Radiochemical Cyclooxygenase-1 and -2 in Vitro Assay for Identification of Natural Products as Inhibitors of Prostaglandin BiosynthesisJ. Nat. Prod., 1998, 61 (1), pp 2–7)
- ↑ M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
- ↑ S.F. Kim Inducible nitric oxide synthase binds, S-nitrosylates, and activates cyclooxygenase-2. Science 2005,310(5756):1966-70)
- ↑ 4.0 4.1 4.2 4.3 4.4 4.5 4.6 [www.ncbi.nlm.nih.gov/pubmed/20463020 Vecchio A. J. "Structural basis of fatty acid substrate binding to cyclooxygenase-2." J. Biol. Chem. 285 22152-63 (2010)]
- ↑ [http://pubs.acs.org/doi/pdf/10.1021/bi035717o Rogge C. "Identification of Tyr504 as an Alternative Tyrosyl Radical Site in Human Prostaglandin H Synthase-2" Biochemistry 2004, 43, 1560-1568]
- ↑ [http://www.jbc.org/content/279/6/4084.full.pdf Bambai B. "Role of Asn-382 and Thr-383 in Activation and Inactivation of Human Prostaglandin H Synthase Cyclooxygenase Catalysis" February 6, 2004 The Journal of Biological Chemistry, 279, 4084-4092.]