Transformation of AA to PGH2

Reaction

AA \rightleftharpoons PGH2

Michaelis-Menten Constants (When AA is the substrate)
Value	Units	Species	Notes	Reference
1.62E-02 ± 0.22E-02	$mM$	Human	Expression Vector: Embryonic kidney cells Enzyme: Cyclooxygenase-2 pH: Not specified Temperature: Not specified	^[1]
5.14E-03 ± 2.90E-04	mM	Mouse	Expression Vector: Baculovirus (Insect Cell) Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.	^[2]
2.1E-03 ± 4.00E-04	mM	Human	Expression Vector: E. Coli Enzyme: Wild Type Cyclooxygenase-2 Enzyme pH: 8.5 Temperature:30 °C	^[3]
2.1E-03 ± 4.00E-04	mM	Human	Expression Vector: Baculuvirus Enzyme: Wild Type Cycloxygenase-2 pH: 7.2 Temperature: 30 °C 100 uM arachidonate substrate,	^[4]

Enzyme Turnover Numbers (When AA is the substrate)
Value	Units	Species	Notes	Reference
1620 ± 24	per minute	Mouse	Expression Vector: Baculovirus (Insect Cell) Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.	^[2]

Michaelis-Menten Constants (When EPA is the substrate)
Value	Units	Species	Notes	Reference
9.45E-03 ± 7.30E-04	mM	Mouse	Expression Vector: Baculovirus (Insect Cell) Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.	^[2]

Enzyme Turnover Numbers (When EPA is the substrate)
Value	Units	Species	Notes	Reference
522 ± 12.6	per minute	Mouse	Expression Vector: Baculovirus (Insect Cell) Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.	^[2]

Michaelis-Menten Constants (When DHA is the substrate)
Value	Units	Species	Notes	Reference
3.68E-02 ± 4.25E-03	mM	Mouse	Expression Vector: Baculovirus (Insect Cell) Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.	^[2]

Enzyme Turnover Numbers (When DHA is the substrate)
Value	Units	Species	Notes	Reference
207 ± 9	per minute	Mouse	Expression Vector: Baculovirus (Insect Cell) Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.	^[2]

Enzyme Concentration
Value	Units	Species	Notes	Reference
1.45e-02	mM	Mouse	Expression Vector: Baculovirus (Insect Cell) Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate.	^[2]

↑ S.F. Kim Inducible nitric oxide synthase binds, S-nitrosylates, and activates cyclooxygenase-2. Science 2005,310(5756):1966-70)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 [www.ncbi.nlm.nih.gov/pubmed/20463020 Vecchio A. J. "Structural basis of fatty acid substrate binding to cyclooxygenase-2." J. Biol. Chem. 285 22152-63 (2010)]
↑ [http://pubs.acs.org/doi/pdf/10.1021/bi035717o Rogge C. "Identification of Tyr504 as an Alternative Tyrosyl Radical Site in Human Prostaglandin H Synthase-2" Biochemistry 2004, 43, 1560-1568]
↑ [http://www.jbc.org/content/279/6/4084.full.pdf Bambai B. "Role of Asn-382 and Thr-383 in Activation and Inactivation of Human Prostaglandin H Synthase Cyclooxygenase Catalysis" February 6, 2004 The Journal of Biological Chemistry, 279, 4084-4092.]