Difference between revisions of "Transformation of AA to PGH2"
(→COX-1 Enzyme Parameters) |
(→COX-2 Enzyme Parameters) |
||
Line 175: | Line 175: | ||
|} | |} | ||
+ | |||
+ | |||
+ | {|class="wikitable sortable" | ||
+ | |+ style="text-align: left;" | COX-1 Abundance | ||
+ | |- | ||
+ | ! Value | ||
+ | ! Units | ||
+ | ! Species | ||
+ | ! Notes | ||
+ | ! Reference | ||
+ | |- | ||
+ | |13.7 | ||
+ | |<math> ppm </math> | ||
+ | |Human | ||
+ | |Expression Vector: Platlet | ||
+ | Enzyme: Cyclooxygenase-2 (PGTS2) | ||
+ | pH: 7.5 | ||
+ | Temperature: 37 °C | ||
+ | |<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref> | ||
+ | |- | ||
+ | |4.11 | ||
+ | |<math> ppm </math> | ||
+ | |Human | ||
+ | |Expression Vector: Stomach | ||
+ | Enzyme: Cyclooxygenase-2 (PGTS2) | ||
+ | pH: 7.5 | ||
+ | Temperature: 37 °C | ||
+ | |<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the | ||
+ | human proteome'' Nature, 2014 509, 582–587]</ref> | ||
+ | |- | ||
+ | |1.49 | ||
+ | |<math> ppm </math> | ||
+ | |Human | ||
+ | |Expression Vector: Oral Cavity | ||
+ | Enzyme: Cyclooxygenase-2 (PGTS2) | ||
+ | pH: 7.5 | ||
+ | Temperature: 37 °C | ||
+ | |<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the | ||
+ | human proteome'' Nature, 2014 509, 582–587]</ref> | ||
+ | |- | ||
+ | |} | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
{|class="wikitable sortable" | {|class="wikitable sortable" |
Revision as of 11:20, 9 August 2016
Contents
Reaction
Chemical equation
Rate equation
COX-1 Enzyme Parameters
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
0.0088 ± 0.0022 | Human | Expression Vector: Human
Enzyme: Cyclooxygenase-1 pH: 7.6 - 8.4 Temperature: 37 °C |
[1] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
1176 | Human | Expression Vector: Urinary Bladder
Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C |
[2] | |
849 | Human | Expression Vector: Platlet
Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C |
[2] | |
208 | Human | Expression Vector: Stomach
Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C |
[3] | |
130 | Human | Expression Vector: Esophagus
Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C |
[3] | |
70.8 | Human | Expression Vector: Oral Cavity
Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C |
[3] | |
19.1 | Human | Expression Vector: Lung
Enzyme: Cyclooxygenase-1 (PGTS1) pH: 7.5 Temperature: 37 °C |
[2] |
COX-2 Enzyme Parameters
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
1.62E-02 ± 0.22E-02 | Human | Expression Vector: Embryonic kidney cells
Enzyme: Cyclooxygenase-2 pH: Not specified Temperature: Not specified |
[4] | |
5.14E-03 ± 2.90E-04 | mM | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[5] |
2.1E-03 ± 4.00E-04 | mM | Human | Expression Vector: E. Coli
Enzyme: Wild Type Cyclooxygenase-2 Enzyme pH: 8.5 Temperature:30 °C |
[6] |
2.1E-03 ± 4.00E-04 | mM | Human | Expression Vector: Baculuvirus
Enzyme: Wild Type Cycloxygenase-2 pH: 7.2 Temperature: 30 °C 100 uM arachidonate substrate, |
[7] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
1620 ± 24 | per minute | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[5] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
13.7 | Human | Expression Vector: Platlet
Enzyme: Cyclooxygenase-2 (PGTS2) pH: 7.5 Temperature: 37 °C |
[2] | |
4.11 | Human | Expression Vector: Stomach
Enzyme: Cyclooxygenase-2 (PGTS2) pH: 7.5 Temperature: 37 °C |
[3] | |
1.49 | Human | Expression Vector: Oral Cavity
Enzyme: Cyclooxygenase-2 (PGTS2) pH: 7.5 Temperature: 37 °C |
[3] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
9.45E-03 ± 7.30E-04 | mM | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[5] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
522 ± 12.6 | per minute | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[5] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
3.68E-02 ± 4.25E-03 | mM | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[5] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
207 ± 9 | per minute | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[5] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
1.45e-02 | mM | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[5] |
References
- ↑ Y. Noreen Development of a Radiochemical Cyclooxygenase-1 and -2 in Vitro Assay for Identification of Natural Products as Inhibitors of Prostaglandin BiosynthesisJ. Nat. Prod., 1998, 61 (1), pp 2–7)
- ↑ 2.0 2.1 2.2 2.3 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
- ↑ 3.0 3.1 3.2 3.3 3.4 [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587]
- ↑ S.F. Kim Inducible nitric oxide synthase binds, S-nitrosylates, and activates cyclooxygenase-2. Science 2005,310(5756):1966-70)
- ↑ 5.0 5.1 5.2 5.3 5.4 5.5 5.6 [www.ncbi.nlm.nih.gov/pubmed/20463020 Vecchio A. J. "Structural basis of fatty acid substrate binding to cyclooxygenase-2." J. Biol. Chem. 285 22152-63 (2010)]
- ↑ [http://pubs.acs.org/doi/pdf/10.1021/bi035717o Rogge C. "Identification of Tyr504 as an Alternative Tyrosyl Radical Site in Human Prostaglandin H Synthase-2" Biochemistry 2004, 43, 1560-1568]
- ↑ [http://www.jbc.org/content/279/6/4084.full.pdf Bambai B. "Role of Asn-382 and Thr-383 in Activation and Inactivation of Human Prostaglandin H Synthase Cyclooxygenase Catalysis" February 6, 2004 The Journal of Biological Chemistry, 279, 4084-4092.]