Difference between revisions of "Transformation of AA to PGH2"
(→COX-2 Enzyme Parameters) |
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|Human | |Human | ||
|Expression Vector: E. Coli | |Expression Vector: E. Coli | ||
− | Enzyme: Wild Type Cyclooxygenase Enzyme | + | Enzyme: Wild Type Cyclooxygenase-2 Enzyme |
pH: 8.5 | pH: 8.5 | ||
Temperature:30 °C | Temperature:30 °C | ||
Line 55: | Line 55: | ||
|Human | |Human | ||
|Expression Vector: Baculuvirus | |Expression Vector: Baculuvirus | ||
− | Enzyme: Wild Type Cycloxygenase | + | Enzyme: Wild Type Cycloxygenase-2 |
pH: 7.2 | pH: 7.2 | ||
Temperature: 30 °C | Temperature: 30 °C |
Revision as of 09:26, 21 July 2016
Contents
Reaction
Chemical equation
Rate equation
COX-2 Enzyme Parameters
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
1.62E-02 ± 0.22E-02 | Human | Expression Vector: Embryonic kidney cells
Enzyme: Cyclooxygenase-2 pH: Not specified Temperature: Not specified |
[1] | |
5.14E-03 ± 2.90E-04 | mM | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[2] |
2.1E-03 ± 4.00E-04 | mM | Human | Expression Vector: E. Coli
Enzyme: Wild Type Cyclooxygenase-2 Enzyme pH: 8.5 Temperature:30 °C |
[3] |
2.1E-03 ± 4.00E-04 | mM | Human | Expression Vector: Baculuvirus
Enzyme: Wild Type Cycloxygenase-2 pH: 7.2 Temperature: 30 °C 100 uM arachidonate substrate, |
[4] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
1620 ± 24 | per minute | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[2] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
9.45E-03 ± 7.30E-04 | mM | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[2] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
522 ± 12.6 | per minute | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[2] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
3.68E-02 ± 4.25E-03 | mM | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[2] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
207 ± 9 | per minute | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[2] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
1.45e-02 | mM | Mouse | Expression Vector: Baculovirus (Insect Cell)
Enzyme: COX-2 pH: 8.0 Temperature: 37 °C 1–200 µM of substrate. |
[2] |
References
- ↑ S.F. Kim Inducible nitric oxide synthase binds, S-nitrosylates, and activates cyclooxygenase-2. Science 2005,310(5756):1966-70)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 [www.ncbi.nlm.nih.gov/pubmed/20463020 Vecchio A. J. "Structural basis of fatty acid substrate binding to cyclooxygenase-2." J. Biol. Chem. 285 22152-63 (2010)]
- ↑ [http://pubs.acs.org/doi/pdf/10.1021/bi035717o Rogge C. "Identification of Tyr504 as an Alternative Tyrosyl Radical Site in Human Prostaglandin H Synthase-2" Biochemistry 2004, 43, 1560-1568]
- ↑ [http://www.jbc.org/content/279/6/4084.full.pdf Bambai B. "Role of Asn-382 and Thr-383 in Activation and Inactivation of Human Prostaglandin H Synthase Cyclooxygenase Catalysis" February 6, 2004 The Journal of Biological Chemistry, 279, 4084-4092.]