Difference between revisions of "46BR.1N Fibroblasts"
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The 46BR.1N human fibroblast cell line was purchased from The European Collection of Cell Culture (ECACC) (Salisbury, UK). This cell line represents immortalised dermal fibroblasts, showing normal cell morphology and function (Arlett et al., 1988). The recommended cell culture medium for 46BR.1N fibroblasts was MEME containing 15% FBS, L-glutamine (200nM), non-essential amino acids (200 nM) and sodium pyruvate (200 nM). | The 46BR.1N human fibroblast cell line was purchased from The European Collection of Cell Culture (ECACC) (Salisbury, UK). This cell line represents immortalised dermal fibroblasts, showing normal cell morphology and function (Arlett et al., 1988). The recommended cell culture medium for 46BR.1N fibroblasts was MEME containing 15% FBS, L-glutamine (200nM), non-essential amino acids (200 nM) and sodium pyruvate (200 nM). | ||
| − | *[[46BR.1N MTT Assay Results|MTT Assay Results]] | + | == Experimental Methods == |
| − | *[[46BR.1N Proteomic Analysis Results|Proteomic Analysis Results]] | + | * [[Materials and Methods|Materials and Methods]] |
| − | *[[46BR.1N Fatty acid analysis Results|Fatty acid analysis Results]] | + | |
| − | *[[46BR.1N Eicosanoid Analysis Results|Eicosanoid Analysis Results]] | + | == Experimental Results == |
| + | *[[46BR.1N fibroblasts MTT Assay Results|MTT Assay Results]] | ||
| + | *[[46BR.1N fibroblasts Proteomic Analysis Results|Proteomic Analysis Results]] | ||
| + | *[[46BR.1N fibroblasts Fatty acid analysis Results|Fatty acid analysis Results]] | ||
| + | *[[46BR.1N fibroblasts Eicosanoid Analysis Results|Eicosanoid Analysis Results]] | ||
| + | |||
| + | == In Silico Experiment Results == | ||
| + | |||
| + | *[[Stimulating 46BR.1N fibroblasts with Calcium Ionophore |Calcium Ionophore]] | ||
| + | *[[Stimulating 46BR.1N fibroblasts with ATP|ATP]] | ||
| + | *[[Stimulating 46BR.1N fibroblasts with UVR|UVR]] | ||
| + | *[[Stimulating COX inhibited 46BR.1N fibroblasts with Calcium Ionophore |COX inhibitor + Calcium Ionophore]] | ||
Revision as of 14:44, 31 May 2019
The 46BR.1N human fibroblast cell line was purchased from The European Collection of Cell Culture (ECACC) (Salisbury, UK). This cell line represents immortalised dermal fibroblasts, showing normal cell morphology and function (Arlett et al., 1988). The recommended cell culture medium for 46BR.1N fibroblasts was MEME containing 15% FBS, L-glutamine (200nM), non-essential amino acids (200 nM) and sodium pyruvate (200 nM).
Experimental Methods
Experimental Results
- MTT Assay Results
- Proteomic Analysis Results
- Fatty acid analysis Results
- Eicosanoid Analysis Results
