Transformation of PL to AA

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Reaction

Catalysed by cPLA2α (3.1.1.4).

Chemical equation

 Phospholipid \rightleftharpoons Arachidonic Acid

Rate equation

cPLA2a Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
2.50E-03  mM Mouse Fibroblasts Cytosolic PLA2 [1]
5.00E-02  mM Human Recombinant PLA2 (Phosphatidylcholine as the substrate) [2]
2.00E-02  mM Human Recombinant PLA2 (Phosphatidylethanolamine as the substrate) [2]
4.13E-01 ± 2.80E-02  mM Rat PMN Neutrophils [3]


Enzyme Turnover Numbers
Value Units Species Notes Reference
2.6E+03± 0.1E+03  per minute Bos taurus Substrate - 1,2-diheptanoyl-sn-glycero-3-phosphorylcholine.

"From the molecular weight, 13,795, the catalytic center activity for the micellar substrate, Kcat{app}, was calculated."

[4]
3000 per minute Porcine Pancreatic phospholipase A, Substrate: 1,2-dipalmitoyl-phosphatidylcholine, Enzyme concentration: 0.2 mg/ml, pH 8.0. [5]
11220 per minute Stingray Secreted, 4 mM NaTDC, 8 mM, CaCl2, pH 8,5 and 40 °C [6]
8400 per minute Camel Pancreatic, 4 mM NaTDC, 8 mM, CaCl2, pH 8,5 and 40 °C [6]
Enzyme Concentrations
Value Units Species Notes Reference
1  mM Porcine Pancreatic phospholipase A, Substrate: 1,2-dipalmitoyl-phosphatidylcholine, Enzyme concentration: 0.2 mg/ml, pH 8.0. [5]
3.7  mM Camel 18kDa protein, 20g of enzyme in 300ml solution.

Pancreatic, 4 mM NaTDC, 8 mM, CaCl2, pH 8,5 and 40 °C

[6]

References

  1. Spaargaren M. “Characterization and identification of an epidermal-growth-factor-activated phospholipase A2.” Biochem J. 1992 Oct 1; 287(Pt 1): 37–43.
  2. 2.0 2.1 Kawauchi Y. “Preparation and characterization of human rheumatoid arthritic synovial fluid phospholipase A2 produced by recombinant baculovirus-infected insect cells.” J Biochem. 1994 Jul;116(1):81-7.
  3. Kawauchi Y. “Changes in kinetic properties of cytosolic phospholipase A2 in activated rat neutrophils.” Adv Exp Med Biol. 1997;433:439-42.
  4. [www.ncbi.nlm.nih.gov/pubmed/8454568 Hada S. “Hydrolysis of Micellar Diheptanoylphosphatidylcholine Catalyzed byBovine Pancreatic Phospholipase A2: Kinetic Characterization of Group I and II Enzymes1” J Biochem (1993) 113 (1): 13-17.]
  5. 5.0 5.1 [http://www.jbc.org/content/261/12/5328.long Menashe M. “Hydrolysis of Dipalmitoylphosphatidylcholine Small Unilamellar Vesicles by Porcine Pancreatic Phospholipase A2” J Biochem (1986) 261, 5328-5333.] Cite error: Invalid <ref> tag; name "Menashe1986" defined multiple times with different content
  6. 6.0 6.1 6.2 Bacha B. “Purification and biochemical characterization of pancreatic phospholipase A2 from the common stingray Dasyatis pastinaca” Lipids Health Dis. 10, 32 (2011)

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