Difference between revisions of "Transformation of AA to PGH2"

From ISMOC
Jump to: navigation, search
(COX-2 Enzyme Parameters)
(COX-2 Enzyme Parameters)
Line 130: Line 130:
 
|207 ± 9
 
|207 ± 9
 
|per minute
 
|per minute
 +
|Mouse
 +
|In vitro, 37 °C, pH 8.0, 1–200 µM of substrate.
 +
|<ref name="Vecchoi2010"> [www.ncbi.nlm.nih.gov/pubmed/20463020 Vecchio A. J. "Structural basis of fatty acid substrate binding to cyclooxygenase-2." J. Biol. Chem. 285 22152-63 (2010)]</ref>
 +
|-
 +
|}
 +
 +
{|class="wikitable sortable"
 +
|+  style="text-align: left;" | Enzyme Concentration
 +
|-
 +
! Value
 +
! Units
 +
! Species
 +
! Notes
 +
! Reference
 +
|-
 +
|1.45e-02
 +
|mM
 
|Mouse
 
|Mouse
 
|In vitro, 37 °C, pH 8.0, 1–200 µM of substrate.
 
|In vitro, 37 °C, pH 8.0, 1–200 µM of substrate.

Revision as of 12:06, 27 April 2016

Return to overview

Reaction

R2 AA-PGH2.jpg

Chemical equation

 AA \rightleftharpoons PGH2

Rate equation

COX-2 Enzyme Parameters

Michaelis-Menten Constants (When AA is the substrate)
Value Units Species Notes Reference
1.62E-02 ± 0.22  mM Human Embryonic kidney cells [1]
5.14E-03 ± 2.90E-04 mM Mouse In vitro, 37 °C, pH 8.0, 1–200 µM of substrate. [2]
2.1E-03 ± 4.00E-04 mM Human Wild Type Enzyme Assay, 30 °C [3]
2.1E-03 ± 4.00E-04 mM Human Wild Type cycloxygenase activty, 100 �M arachidonate substrate, pH 7.2. [4]
Enzyme Turnover Numbers (When AA is the substrate)
Value Units Species Notes Reference
1620 ± 24 per minute Mouse In vitro, 37 °C, pH 8.0, 1–200 µM of substrate. [2]
Michaelis-Menten Constants (When EPA is the substrate)
Value Units Species Notes Reference
9.45E-03 ± 7.30E-04 mM Mouse In vitro, 37 °C, pH 8.0, 1–200 µM of substrate. [2]
Enzyme Turnover Numbers (When EPA is the substrate)
Value Units Species Notes Reference
522 ± 12.6 per minute Mouse In vitro, 37 °C, pH 8.0, 1–200 µM of substrate. [2]
Michaelis-Menten Constants (When DHA is the substrate)
Value Units Species Notes Reference
3.68E-02 ± 4.25E-03 mM Mouse In vitro, 37 °C, pH 8.0, 1–200 µM of substrate. [2]
Enzyme Turnover Numbers (When DHA is the substrate)
Value Units Species Notes Reference
207 ± 9 per minute Mouse In vitro, 37 °C, pH 8.0, 1–200 µM of substrate. [2]
Enzyme Concentration
Value Units Species Notes Reference
1.45e-02 mM Mouse In vitro, 37 °C, pH 8.0, 1–200 µM of substrate. [2]

References

  1. S.F. Kim Inducible nitric oxide synthase binds, S-nitrosylates, and activates cyclooxygenase-2. Science 2005,310(5756):1966-70)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 [www.ncbi.nlm.nih.gov/pubmed/20463020 Vecchio A. J. "Structural basis of fatty acid substrate binding to cyclooxygenase-2." J. Biol. Chem. 285 22152-63 (2010)]
  3. [http://pubs.acs.org/doi/pdf/10.1021/bi035717o Rogge C. "Identification of Tyr504 as an Alternative Tyrosyl Radical Site in Human Prostaglandin H Synthase-2" Biochemistry 2004, 43, 1560-1568]
  4. [http://www.jbc.org/content/279/6/4084.full.pdf Bambai B. "Role of Asn-382 and Thr-383 in Activation and Inactivation of Human Prostaglandin H Synthase Cyclooxygenase Catalysis" February 6, 2004 The Journal of Biological Chemistry, 279, 4084-4092.]

Related Reactions