Transformation of PGJ2 to exPGJ2

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Rate equation

Transport Parameters

K_ms

Literature values

Value	Units	Species	Notes	Weight	Reference
0.0109 ± 0.0041	mM	Human	Substrate: LTC4 pH 7.0, 37°C, recombinant MRP2, using 4mM MgATP and 5g of isolated membranes.	512
0.0000366 ± 0.0000038	mM	Human	Substrate LTC4, 37 °C, 4 mM ATP,	128
5.3E-3 ± 2.6E-3	mM	Human	4 mM ATP, 37°C, 5–10 ml of membrane vesicle suspension (30 mg protein).	128
0.1954 ± 0.0612	mM	Human	pH 7.0, 37°C, recombinant MRP2, Substrate: 17beta-estradiol 17-(beta-D-glucuronide)	512
0.1097 ± 0.0391	mM	Human	pH 7.0, 37°C, recombinant MRP2, Substrate: Estrone glucuronide	512
0.0207	mM	Human	pH 7.4, 37°C, Substrate: Progesterone, Expression vector: Human HepG2 hepatoma	2048

Description of the ABC Kms distribution

Mode (mM)	Confidence Interval	Location parameter (µ)	Scale parameter (σ)
1.13E+01	1.00E+01	3.22E+00	8.91E-01

The estimated probability distribution for ABC Kms. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

k_cat

No data available on the turnover of ABCC4 transporter therefore based upon estimates made by http://book.bionumbers.org/what-are-the-rates-of-membrane-transporters/

Literature values

Value	Units	Transporter	Notes	Weight	Reference
3.5	min-1	Glucose transporter ptsI	Organism: Bacteria Escherichia coli Back of the envelope calculation by BioNumbers: http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=102931	8
0.33 - 0.83	min-1	Rate of transport of H+/Lactose transporter	Organism: Bacteria Escherichia coli Back of the envelop calculation on bionumbers http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=103159	8
3.28	min-1	Catalytic Rate of transporter HXT7	Organism: Budding yeast Saccharomyces cerevisiae Back of the envelope calculation http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=101737	8
11.34	min-1	Human	pH 7.0, 37°C, recombinant MRP2, Substrate: Estrone glucuronide	8

Description of the ABC kcat distribution

Mode (min-1)	Confidence Interval	Location parameter (µ)	Scale parameter (σ)
2.10E-02	2.37E+01	-2.60E+00	1.12E+00

The estimated probability distribution for ABC kcat. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

V_max

Literature values

Value	Units	Species	Notes	Reference
0.00019 ± 1.96e-5	mmol/min/mg protein	Human	Substrate: LTC4 pH 7.0, 37°C, recombinant MRP2, using 4mM MgATP and 5g of isolated membranes.
1.25e-7 ± 1.2e-8	mmol/min/mg	Human	Substrate LTC4, 37 °C, 4 mM ATP,
2.02e-8 ± 5.9e-9	mmol/mg/min	Human	4 mM ATP, 37°C, 5–10 ml of membrane vesicle suspension (30 mg protein).

Transporter concentration

To convert the enzyme concentration from ppm to mM, the following equation was used.

Literature values

Value	Units	Species	Notes	Weight	Reference
5.15	ppm	Human	Expression Vector: Stomach Enzyme: ABCC4 pH: 7.5 Temperature: 37 °C	2048
5.94	ppm	Human	Expression Vector: Lung Enzyme: ABCC4 pH: 7.5 Temperature: 37 °C	2048
2.66	ppm	Human	Expression Vector: Gut Enzyme: ABCC4 pH: 7.5 Temperature: 37 °C	2048

Description of the ABC concentration distribution

Mode (ppm)	Mode (mM)	Confidence Interval	Location parameter (µ)	Scale parameter (σ)
5.06E+00	2.80E-05	1.44E+00	1.74E+00	3.42E-01

The estimated probability distribution for ABC concentration. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.