Transformation of PGH2 to PGF2α

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PGF2α has been found to be produced by keratinocyte cells in vitro (Rhodes, Gledhill et al. 2009). This species is synthesised from PGH2 via the prostaglandin F synthase (PGFS) or the PGE 9-ketoreductase enzymes. Upon production this species have been associated with pigmentation, inflammation and hair growth in the skin (Scott, Leopardi et al. 2004, Scott, Fricke et al. 2007). The species has various inflammatory roles such as a pro-inflammatory effect upon the system by facilitating pain transmission (Murata, Ushikubi et al. 1997) and also an anti-inflammatory effect by inhibiting lymphocyte and endothelial cell interactions (Della Bella, Molteni et al. 2001).

Reaction

Chemical equation

$PGH2 \rightleftharpoons PGF2a$

Rate equation

Parameters

Michaelis-Menten Constants

Value	Units	Species	Notes	Reference
1.90E-03 ± 1.5E-03	$mM$	Human	Placental Aldose Reductase (AKR1B1)
1.80E-02	$mM$	Human	Lung PGF2a Synthase (AKR1C3)

Enzyme Turnover Numbers

Value	Units	Species	Notes	Reference
14.9	per minute	Mouse/Swine	Prostamide/PGF Synthase Purified from Swine Brain and of Murine Enzyme Expressed in E. coli

PGFS Abundance

Value	Units	Species	Notes	Reference
105	$ppm$	Human	Expression Vector: Platelet Enzyme: PGFS pH: 7.5 Temperature: 37 °C
19.9	$ppm$	Human	Expression Vector: Lung Enzyme: PGFS pH: 7.5 Temperature: 37 °C
40.3	$ppm$	Human	Expression Vector: Esophagus Enzyme: PGFS pH: 7.5 Temperature: 37 °C
110	$ppm$	Human	Expression Vector: Oral Cavity Enzyme: PGFS pH: 7.5 Temperature: 37 °C

Related Reactions

• Transformation of AA to PGH2