Difference between revisions of "Transformation of PGH2 to PGF2α"

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|+  style="text-align: left;" | Enzyme Concentrations
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|+  style="text-align: left;" | PGFS Abundance
 
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! Value
 
! Value
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! Reference
 
! Reference
 
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|-
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|0.00267
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|105
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|mM
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|<math> ppm </math>
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|Human
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|Human  
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|Calculated by 20ug of 20 kDa enzyme in 300uL solution.
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|Expression Vector: Platelet
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|<ref name="Kabututu2009"> [http://www.ncbi.nlm.nih.gov/pubmed/19010934 Kabututu Z. "Prostaglandin F2alpha synthase activities of aldo-keto reductase 1B1, 1B3 and 1B7.'' J Biochem. 2009 Feb;145(2):161-8. doi: 10.1093/jb/mvn152. Epub 2008 Nov 14.]</ref>
+
Enzyme: PGFS
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 +
|-
 +
|19.9
 +
|<math> ppm </math>
 +
|Human
 +
|Expression Vector: Lung
 +
Enzyme: PGFS
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
 +
|-
 +
|40.3
 +
|<math> ppm </math>
 +
|Human
 +
|Expression Vector: Esophagus
 +
Enzyme: PGFS
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
 +
|-
 +
|110
 +
|<math> ppm </math>
 +
|Human
 +
|Expression Vector: Oral Cavity
 +
Enzyme: PGFS
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
 +
|-
 
|}
 
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Revision as of 11:59, 9 August 2016

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Reaction

R3 PGH2 -PGF2A.jpg

Chemical equation

FA \rightleftharpoons AA

Rate equation

Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
1.90E-03 ± 1.5E-03 mM Human Placental Aldose Reductase (AKR1B1) [1]
1.80E-02 mM Human Lung PGF2a Synthase (AKR1C3) [1]
Enzyme Turnover Numbers
Value Units Species Notes Reference
14.9 per minute Mouse/Swine Prostamide/PGF Synthase Purified

from Swine Brain and of Murine Enzyme Expressed in E. coli

[2]
PGFS Abundance
Value Units Species Notes Reference
105 ppm Human Expression Vector: Platelet

Enzyme: PGFS pH: 7.5 Temperature: 37 °C

[3]
19.9 ppm Human Expression Vector: Lung

Enzyme: PGFS pH: 7.5 Temperature: 37 °C

[4]
40.3 ppm Human Expression Vector: Esophagus

Enzyme: PGFS pH: 7.5 Temperature: 37 °C

[4]
110 ppm Human Expression Vector: Oral Cavity

Enzyme: PGFS pH: 7.5 Temperature: 37 °C

[4]

References

  1. ↑ 1.0 1.1 Z. Kabututu, M. Manin, Prostaglandin F2alpha synthase activities of aldo-keto reductase 1B1, 1B3 and 1B7. J Biochem 2009,145(2):161-8)
  2. ↑ Moriuchi H., Molecular characterization of a novel type of prostamide/prostaglandin F synthase, belonging to the thioredoxin-like superfamily. J Biol Chem. 2008 Jan 11;283(2):792-801.)
  3. ↑ M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
  4. ↑ 4.0 4.1 4.2 M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587


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