Difference between revisions of "Transformation of PGH2 to PGF2α"

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(Parameters)
(Parameters)
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from Swine Brain and of Murine Enzyme Expressed in E. coli
 
from Swine Brain and of Murine Enzyme Expressed in E. coli
 
| <ref name="Moriuchi2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18006499 Moriuchi H., Molecular characterization of a novel type of prostamide/prostaglandin F synthase, belonging to the thioredoxin-like superfamily.'' J Biol Chem. 2008 Jan 11;283(2):792-801.)]</ref>  
 
| <ref name="Moriuchi2008"> [http://www.ncbi.nlm.nih.gov/pubmed/18006499 Moriuchi H., Molecular characterization of a novel type of prostamide/prostaglandin F synthase, belonging to the thioredoxin-like superfamily.'' J Biol Chem. 2008 Jan 11;283(2):792-801.)]</ref>  
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{|class="wikitable sortable"
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|+  style="text-align: left;" | Enzyme Concentrations
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|-
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! Value
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! Units
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! Species
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! Notes
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! Reference
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|0.00267
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|mM
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|Human
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|Calculated by 20ug of 20 kDa enzyme in 300uL solution.
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|<ref name="Kabututu2009"> [http://www.ncbi.nlm.nih.gov/pubmed/19010934 Kabututu Z. "Prostaglandin F2alpha synthase activities of aldo-keto reductase 1B1, 1B3 and 1B7.'' J Biochem. 2009 Feb;145(2):161-8. doi: 10.1093/jb/mvn152. Epub 2008 Nov 14.]</ref>
 
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Revision as of 11:31, 27 April 2016

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Reaction

R3 PGH2 -PGF2A.jpg

Chemical equation

 FA \rightleftharpoons AA

Rate equation

Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
1.90E-03 ± 1.5E-03  mM Human Placental Aldose Reductase (AKR1B1) [1]
1.80E-02  mM  Human Lung PGF2a Synthase (AKR1C3) [1]
Enzyme Turnover Numbers
Value Units Species Notes Reference
14.9 per minute Mouse/Swine Prostamide/PGF Synthase Purified

from Swine Brain and of Murine Enzyme Expressed in E. coli

[2]
Enzyme Concentrations
Value Units Species Notes Reference
0.00267 mM Human Calculated by 20ug of 20 kDa enzyme in 300uL solution. [1]

References


Related Reactions