Transformation of PGH2 to PGE2

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Reaction

R10 PGH2 - PGE2.jpg

Chemical equation

FA \rightleftharpoons AA

Rate equation

PGES Enzyme Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
1.6E-01 ± 4.00E-03 mM Human Synovial Tissue [1]
2.15E-01 mM Human Wild Type Enzyme [2]
1.49E-02 mM Human cPGES, casein kinase II and Hsp90 [3]
6.66E-02 mM Human cPGES [3]
Enzyme Turnover Numbers
Value Units Species Notes Reference
3000 ± 360 per minute Human Microsomal Prostaglandin E Synthase 1 expressed in E. Coli. [4]
Enzyme Concentrations
Value Units Species Notes Reference
0.00002353 mM Human 40ng/100ul of a 17 kDa protein

Synovial Tissue

[1]
0.47059 mM Human 8 mg/mL of 17kDa of protien [2]

References

  1. ↑ 1.0 1.1 Pettersson P. , "Identification of beta-trace as prostaglandin D synthase. FASEB J. 2010 Dec;24(12):4668-77. doi: 10.1096/fj.10-164863. Epub 2010 Jul 28.
  2. ↑ 2.0 2.1 Hamza A. , "Understanding microscopic binding of human microsomal prostaglandin E synthase-1 (mPGES-1) trimer with substrate PGH2 and cofactor GSH: insights from computational alanine scanning and site-directed mutagenesis. J Phys Chem B. 2010 Apr 29;114(16):5605-16. doi: 10.1021/jp100668y.
  3. ↑ 3.0 3.1 Kobayashi T. , "Regulation of cytosolic prostaglandin E synthase by phosphorylation. Biochem J. 2004 Jul 1;381(Pt 1):59-69.
  4. ↑ [www.ncbi.nlm.nih.gov/pubmed/16399384 Pettersson P., "Human microsomal prostaglandin E synthase 1: a member of the MAPEG protein superfamily. Methods Enzymol. 2005;401:147-61.]


Related Reactions

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