Difference between revisions of "Transformation of PGH2 to PGE2"
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| Line 24: | Line 24: | ||
|<math> mM </math> | |<math> mM </math> | ||
|Human | |Human | ||
| − | | | + | |Expression Vector: E. Coli |
| + | Enzyme: PGES | ||
| + | pH: 8 | ||
| + | Temperature: 37 | ||
|<ref name="Pettersson2005"> [http://www.ncbi.nlm.nih.gov/pubmed/16399384 Pettersson P. , "Identification of beta-trace as prostaglandin D synthase.'' FASEB J. 2010 Dec;24(12):4668-77. doi: 10.1096/fj.10-164863. Epub 2010 Jul 28.]</ref> | |<ref name="Pettersson2005"> [http://www.ncbi.nlm.nih.gov/pubmed/16399384 Pettersson P. , "Identification of beta-trace as prostaglandin D synthase.'' FASEB J. 2010 Dec;24(12):4668-77. doi: 10.1096/fj.10-164863. Epub 2010 Jul 28.]</ref> | ||
|- | |- | ||
| Line 36: | Line 39: | ||
|<math> mM </math> | |<math> mM </math> | ||
|Human | |Human | ||
| − | |cPGES, casein kinase II and Hsp90 | + | |Expression Vector: E. Coli |
| + | Enzyme: PGES | ||
| + | pH: Unknown | ||
| + | Temperature: Unknown | ||
| + | |||
| + | Other: cPGES, casein kinase II and Hsp90 | ||
|<ref name="Kobayashi04"> [http://www.ncbi.nlm.nih.gov/pubmed/15040786 Kobayashi T. , "Regulation of cytosolic prostaglandin E synthase by phosphorylation.'' Biochem J. 2004 Jul 1;381(Pt 1):59-69.]</ref> | |<ref name="Kobayashi04"> [http://www.ncbi.nlm.nih.gov/pubmed/15040786 Kobayashi T. , "Regulation of cytosolic prostaglandin E synthase by phosphorylation.'' Biochem J. 2004 Jul 1;381(Pt 1):59-69.]</ref> | ||
|- | |- | ||
| Line 42: | Line 50: | ||
|<math> mM </math> | |<math> mM </math> | ||
|Human | |Human | ||
| − | | | + | |Expression Vector: E. Coli |
| + | Enzyme: PGES | ||
| + | pH: Unknown | ||
| + | Temperature: Unknown | ||
|<ref name="Kobayashi04"></ref> | |<ref name="Kobayashi04"></ref> | ||
|- | |- | ||
Revision as of 14:59, 28 May 2016
Contents
Reaction
Chemical equation
Rate equation
PGES Enzyme Parameters
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 1.6E-01 ± 4.00E-03 | 
|
Human | Expression Vector: E. Coli
Enzyme: PGES pH: 8 Temperature: 37 |
[1] |
| 2.15E-01 |
|
Human | Wild Type Enzyme | [2] |
| 1.49E-02 |
|
Human | Expression Vector: E. Coli
Enzyme: PGES pH: Unknown Temperature: Unknown Other: cPGES, casein kinase II and Hsp90 |
[3] |
| 6.66E-02 |
|
Human | Expression Vector: E. Coli
Enzyme: PGES pH: Unknown Temperature: Unknown |
[3] |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 3000 ± 360 | per minute | Human | Microsomal Prostaglandin E Synthase 1 expressed in E. Coli. | [4] |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 0.00002353 | mM | Human | 40ng/100ul of a 17 kDa protein
Synovial Tissue |
[1] |
| 0.47059 | mM | Human | 8 mg/mL of 17kDa of protien | [2] |
References
- ↑ 1.0 1.1 Pettersson P. , "Identification of beta-trace as prostaglandin D synthase. FASEB J. 2010 Dec;24(12):4668-77. doi: 10.1096/fj.10-164863. Epub 2010 Jul 28.
- ↑ 2.0 2.1 Hamza A. , "Understanding microscopic binding of human microsomal prostaglandin E synthase-1 (mPGES-1) trimer with substrate PGH2 and cofactor GSH: insights from computational alanine scanning and site-directed mutagenesis. J Phys Chem B. 2010 Apr 29;114(16):5605-16. doi: 10.1021/jp100668y.
- ↑ 3.0 3.1 Kobayashi T. , "Regulation of cytosolic prostaglandin E synthase by phosphorylation. Biochem J. 2004 Jul 1;381(Pt 1):59-69.
- ↑ [www.ncbi.nlm.nih.gov/pubmed/16399384 Pettersson P., "Human microsomal prostaglandin E synthase 1: a member of the MAPEG protein superfamily. Methods Enzymol. 2005;401:147-61.]
