Difference between revisions of "Transformation of PGH2 to PGE2"

Revision as of 17:11, 26 April 2016

FA \rightleftharpoons AA

Michaelis-Menten Constants
Value	Units	Species	Notes	Reference
1.6E-01 ± 4.00E-03	$mM$	Human	Synovial Tissue	^[1]
2.15E-01	$mM$	Human	Wild Type Enzyme	^[2]
1.49E-02	$mM$	Human	cPGES, casein kinase II and Hsp90	^[3]
6.66E-02	$mM$	Human	cPGES	^[3]

Enzyme Turnover Numbers
Value	Units	Species	Notes	Reference
3000 ± 360	per minute	Human	Microsomal Prostaglandin E Synthase 1 expressed in E. Coli.	^[4]

Enzyme Concentrations
Value	Units	Species	Notes	Reference
0.00002353	mM	Human	40ng/100ul of a 17 kDa protein Synovial Tissue	^[1]
0.47059	mM	Human	8 mg/mL of 17kDa of protien	^[2]

@@ Line 56: / Line 56: @@
 ! Reference
 |-
-| 3000
+| 3000 ± 360
 | per minute
 | Human
 | Microsomal Prostaglandin E Synthase 1 expressed in E. Coli.
 | <ref name="Pettersson20005"> [www.ncbi.nlm.nih.gov/pubmed/16399384 Pettersson P., "Human microsomal prostaglandin E synthase 1: a member of the MAPEG protein superfamily.'' Methods Enzymol. 2005;401:147-61.]</ref>
+|-
+|}
+{|class="wikitable sortable"
+|+  style="text-align: left;" | Enzyme Concentrations
+|-
+! Value
+! Units
+! Species
+! Notes
+! Reference
+|-
+|0.00002353
+|mM
+|Human
+|40ng/100ul of a 17 kDa protein
+Synovial Tissue
+|<ref name="Pettersson2005"> [http://www.ncbi.nlm.nih.gov/pubmed/16399384 Pettersson P. , "Identification of beta-trace as prostaglandin D synthase.'' FASEB J. 2010 Dec;24(12):4668-77. doi: 10.1096/fj.10-164863. Epub 2010 Jul 28.]</ref>
+|-
+|0.47059
+|mM
+|Human
+|8 mg/mL of 17kDa of protien
+|<ref name="Hamza2010"> [http://www.ncbi.nlm.nih.gov/pubmed/20369883 Hamza A. , "Understanding microscopic binding of human microsomal prostaglandin E synthase-1 (mPGES-1) trimer with substrate PGH2 and cofactor GSH: insights from computational alanine scanning and site-directed mutagenesis.'' J Phys Chem B. 2010 Apr 29;114(16):5605-16. doi: 10.1021/jp100668y.]</ref>
+|-
 |}