Transformation of PGH2 to PGD2

Reaction

FA \rightleftharpoons AA

Michaelis-Menten Constants
Value	Units	Species	Notes	Reference
4.00E-03	$mM$	Human	Expression Vector: Human Cell Enzyme: PGDS pH: 8 Temperature: 25	^[1]
4.00E-03	$mM$	Human	Expression Vector: Cerebrospinal Fluid Enzyme: PGDS pH:10 Temperature: Unspecified	^[2]

Enzyme Turnover Numbers
Value	Units	Species	Notes	Reference
158.4	per minute	Human	Expression Vector: Human Cell Enzyme: PGDS pH: 8 Temperature: 25	^[1]

PGDS Abundance
Value	Units	Species	Notes	Reference
156	$ppm$	Human	Expression Vector: Pancreas Enzyme: PGDS pH: 7.5 Temperature: 37 °C	^[3]
101	$ppm$	Human	Expression Vector: Oral Cavity Enzyme: PGDS pH: 7.5 Temperature: 37 °C	^[4]
67.9	$ppm$	Human	Expression Vector: Esophagus Enzyme: PGDS pH: 7.5 Temperature: 37 °C	^[3]
44.5	$ppm$	Human	Expression Vector:Skin Enzyme: PGDS pH: 7.5 Temperature: 37 °C	^[4]

Gibbs Free Energy Change
Value	Units	Species	Notes	Reference
	$kJ/mol$	Human	Expression Vector: E. Coli Enzyme: L-PGDS pH: 8 Temperature: 25	^[5]
5.72	kcal/mol	Not stated	Estimated Enzyme: PGDS Substrate: Arachidonate Product: PGD2 pH: 7.3 ionic strength: 0.25	^[6]

↑ ^1.0 ^1.1 [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Zhou Y. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules. FASEB J. 2010 Dec;24(12):4668-77. doi: 10.1096/fj.10-164863. Epub 2010 Jul 28. ] Cite error: Invalid <ref> tag; name "Zhou2010" defined multiple times with different content
↑ [http://www.ncbi.nlm.nih.gov/pubmed/8093029 Watanabe K. , "Identification of beta-trace as prostaglandin D synthase. Biochem Biophys Res Commun. 1994 Sep 15;203(2):1110-6. ]
↑ ^3.0 ^3.1 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
↑ ^4.0 ^4.1 M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587
↑ [http://ac.els-cdn.com/S0014579314000982/1-s2.0-S0014579314000982-main.pdf?_tid=7f323a24-6543-11e6-82ac-00000aab0f01&acdnat=1471525305_289e200c1e492c5fa693179a5396fd82 Kume S., "Fine-tuned broad binding capability of human lipocalin-type prostaglandin D synthase for various small lipophilic ligands FEBS Letters Volume 588, Issue 6, 18 March 2014, Pages 962–969]
↑ Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471