Difference between revisions of "Transformation of PGH2 to PGD2"

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(Parameters)
(Enzyme Parameters)
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|+  style="text-align: left;" | Enzyme Concentrations
 
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! Value
 
! Units
 
! Species
 
! Notes
 
! Reference
 
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|0.000256
 
|mM
 
|Human
 
|Expression Vector: Cerebrospinal Fluid
 
Enzyme: PGDS
 
pH:10
 
Temperature: Unspecified
 
  
Calculated:
 
20 ug/3ml of a 26 kDa protein.
 
|<ref name="Watanabe1994"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Watanabe K. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' Biochem Biophys Res Commun. 1994 Sep 15;203(2):1110-6.]</ref>
 
|-
 
|0.769
 
|mM
 
|Human
 
|Expression Vector: Human Cell
 
Enzyme: PGDS
 
pH: 8
 
Temperature: 25
 
 
Calculated: 20mg/ml of 26 kDa protein
 
|<ref name="Zhou2010"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Zhou Y. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' FASEB J. 2010 Dec;24(12):4668-77.]</ref>
 
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Revision as of 13:29, 12 August 2016

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R21 PGH2 - PGD2.jpg

Chemical equation

 FA \rightleftharpoons AA

Rate equation

Enzyme Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
4.00E-03  mM Human Expression Vector: Human Cell

Enzyme: PGDS pH: 8 Temperature: 25

[1]
4.00E-03  mM Human Expression Vector: Cerebrospinal Fluid

Enzyme: PGDS pH:10 Temperature: Unspecified

[2]
Enzyme Turnover Numbers
Value Units Species Notes Reference
158.4 per minute Human Expression Vector: Human Cell

Enzyme: PGDS pH: 8 Temperature: 25

[1]


PGDS Abundance
Value Units Species Notes Reference
156  ppm Human Expression Vector: Pancreas

Enzyme: PGDS pH: 7.5 Temperature: 37 °C

[3]
101  ppm Human Expression Vector: Oral Cavity

Enzyme: PGDS pH: 7.5 Temperature: 37 °C

[4]
67.9  ppm Human Expression Vector: Esophagus

Enzyme: PGDS pH: 7.5 Temperature: 37 °C

[3]
44.5  ppm Human Expression Vector:Skin

Enzyme: PGDS pH: 7.5 Temperature: 37 °C

[4]

References

  1. 1.0 1.1 [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Zhou Y. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules. FASEB J. 2010 Dec;24(12):4668-77. doi: 10.1096/fj.10-164863. Epub 2010 Jul 28. ] Cite error: Invalid <ref> tag; name "Zhou2010" defined multiple times with different content
  2. [http://www.ncbi.nlm.nih.gov/pubmed/8093029 Watanabe K. , "Identification of beta-trace as prostaglandin D synthase. Biochem Biophys Res Commun. 1994 Sep 15;203(2):1110-6. ]
  3. 3.0 3.1 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
  4. 4.0 4.1 M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587

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