Difference between revisions of "Transformation of PGH2 to PGD2"

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== Parameters ==
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== Enzyme Parameters ==
 
{|class="wikitable sortable"  
 
{|class="wikitable sortable"  
 
|+  style="text-align: left;" | Michaelis-Menten Constants  
 
|+  style="text-align: left;" | Michaelis-Menten Constants  
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Calculated: 20mg/ml of 26 kDa protein
 
Calculated: 20mg/ml of 26 kDa protein
 
|<ref name="Zhou2010"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Zhou Y. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' FASEB J. 2010 Dec;24(12):4668-77.]</ref>  
 
|<ref name="Zhou2010"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Zhou Y. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' FASEB J. 2010 Dec;24(12):4668-77.]</ref>  
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{|class="wikitable sortable"
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|+  style="text-align: left;" | PGDS Abundance
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|-
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! Value
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! Units
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! Species
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! Notes
 +
! Reference
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|-
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|156
 +
|<math> ppm </math>
 +
|Human
 +
|Expression Vector: Pancreas
 +
Enzyme: PGDS
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 +
|-
 +
|101
 +
|<math> ppm </math>
 +
|Human
 +
|Expression Vector: Oral Cavity
 +
Enzyme: PGDS
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
 +
|-
 +
|67.9
 +
|<math> ppm </math>
 +
|Human
 +
|Expression Vector: Esophagus
 +
Enzyme: PGDS
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 +
|-
 +
|44.5
 +
|<math> ppm </math>
 +
|Human
 +
|Expression Vector:Skin
 +
Enzyme: PGDS
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref>
 
|-
 
|-
 
|}
 
|}

Revision as of 11:43, 9 August 2016

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R21 PGH2 - PGD2.jpg

Chemical equation

FA \rightleftharpoons AA

Rate equation

Enzyme Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
4.00E-03 mM Human Expression Vector: Human Cell

Enzyme: PGDS pH: 8 Temperature: 25

[1]
4.00E-03 mM Human Expression Vector: Cerebrospinal Fluid

Enzyme: PGDS pH:10 Temperature: Unspecified

[2]
Enzyme Turnover Numbers
Value Units Species Notes Reference
158.4 per minute Human Expression Vector: Human Cell

Enzyme: PGDS pH: 8 Temperature: 25

[1]
Enzyme Concentrations
Value Units Species Notes Reference
0.000256 mM Human Expression Vector: Cerebrospinal Fluid

Enzyme: PGDS pH:10 Temperature: Unspecified

Calculated: 20 ug/3ml of a 26 kDa protein.

[2]
0.769 mM Human Expression Vector: Human Cell

Enzyme: PGDS pH: 8 Temperature: 25

Calculated: 20mg/ml of 26 kDa protein

[1]
PGDS Abundance
Value Units Species Notes Reference
156 ppm Human Expression Vector: Pancreas

Enzyme: PGDS pH: 7.5 Temperature: 37 °C

[3]
101 ppm Human Expression Vector: Oral Cavity

Enzyme: PGDS pH: 7.5 Temperature: 37 °C

[4]
67.9 ppm Human Expression Vector: Esophagus

Enzyme: PGDS pH: 7.5 Temperature: 37 °C

[3]
44.5 ppm Human Expression Vector:Skin

Enzyme: PGDS pH: 7.5 Temperature: 37 °C

[4]

References

  1. ↑ 1.0 1.1 1.2 [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Zhou Y. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules. FASEB J. 2010 Dec;24(12):4668-77. doi: 10.1096/fj.10-164863. Epub 2010 Jul 28. ] Cite error: Invalid <ref> tag; name "Zhou2010" defined multiple times with different content Cite error: Invalid <ref> tag; name "Zhou2010" defined multiple times with different content
  2. ↑ 2.0 2.1 [http://www.ncbi.nlm.nih.gov/pubmed/8093029 Watanabe K. , "Identification of beta-trace as prostaglandin D synthase. Biochem Biophys Res Commun. 1994 Sep 15;203(2):1110-6. ] Cite error: Invalid <ref> tag; name "Watanabe1994" defined multiple times with different content
  3. ↑ 3.0 3.1 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
  4. ↑ 4.0 4.1 M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587

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