Difference between revisions of "Transformation of PGH2 to PGD2"

Revision as of 14:24, 28 May 2016

FA \rightleftharpoons AA

Michaelis-Menten Constants
Value	Units	Species	Notes	Reference
4.00E-03	$mM$	Human	Expression Vector: Human Cell Enzyme: PGDS pH: 8 Temperature: 25	^[1]
4.00E-03	$mM$	Human	Expression Vector: Cerebrospinal Fluid Enzyme: PGDS pH:10 Temperature: Unspecified	^[2]

Enzyme Turnover Numbers
Value	Units	Species	Notes	Reference
158.4	per minute	Human	Wild type (Paper includes 13 mutants of the enzyme)	^[1]

Enzyme Concentrations
Value	Units	Species	Notes	Reference
0.000256	mM	Human	Expression Vector: Cerebrospinal Fluid Enzyme: PGDS pH:10 Temperature: Unspecified Calculated: 20 ug/3ml of a 26 kDa protein.	^[2]
0.769	mM	Human	Expression Vector: Human Cell Enzyme: PGDS pH: 8 Temperature: 25 Calculated: 20mg/ml of 26 kDa protein	^[1]

↑ ^1.0 ^1.1 ^1.2 [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Zhou Y. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules. FASEB J. 2010 Dec;24(12):4668-77. doi: 10.1096/fj.10-164863. Epub 2010 Jul 28. ] Cite error: Invalid <ref> tag; name "Zhou2010" defined multiple times with different content Cite error: Invalid <ref> tag; name "Zhou2010" defined multiple times with different content
↑ ^2.0 ^2.1 [http://www.ncbi.nlm.nih.gov/pubmed/8093029 Watanabe K. , "Identification of beta-trace as prostaglandin D synthase. Biochem Biophys Res Commun. 1994 Sep 15;203(2):1110-6. ] Cite error: Invalid <ref> tag; name "Watanabe1994" defined multiple times with different content

@@ Line 72: / Line 72: @@
 |mM
 |Human
-Expression Vector: Cerebrospinal Fluid
+|Expression Vector: Cerebrospinal Fluid
 Enzyme: PGDS
 pH:10