Difference between revisions of "Transformation of PGH2 to PGD2"
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| Line 23: | Line 23: | ||
|<math> mM </math> | |<math> mM </math> | ||
|Human | |Human | ||
| − | | | + | |Expression Vector: Human Cell |
| − | |<ref name=" | + | Enzyme: PGDS |
| + | pH: 8 | ||
| + | Temperature: 25 | ||
| + | |<ref name="Zhou2010"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Zhou Y. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' FASEB J. 2010 Dec;24(12):4668-77. doi: 10.1096/fj.10-164863. Epub 2010 Jul 28. | ||
| + | ]</ref> | ||
|- | |- | ||
|4.00E-03 | |4.00E-03 | ||
|<math> mM </math> | |<math> mM </math> | ||
|Human | |Human | ||
| − | | | + | |Expression Vector: Cerebrospinal Fluid |
| − | |<ref name=" | + | Enzyme: PGDS |
| + | pH:10 | ||
| + | Temperature: Unspecified | ||
| + | |||
| + | |<ref name="Watanabe1994"> [http://www.ncbi.nlm.nih.gov/pubmed/8093029 Watanabe K. , "Identification of beta-trace as prostaglandin D synthase.'' | ||
| + | Biochem Biophys Res Commun. 1994 Sep 15;203(2):1110-6. ]</ref> | ||
|- | |- | ||
| Line 63: | Line 72: | ||
|mM | |mM | ||
|Human | |Human | ||
| − | + | Expression Vector: Cerebrospinal Fluid | |
| + | Enzyme: PGDS | ||
| + | pH:10 | ||
| + | Temperature: Unspecified | ||
| + | |||
| + | Calculated: | ||
| + | 20 ug/3ml of a 26 kDa protein. | ||
|<ref name="Watanabe1994"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Watanabe K. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' Biochem Biophys Res Commun. 1994 Sep 15;203(2):1110-6.]</ref> | |<ref name="Watanabe1994"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Watanabe K. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' Biochem Biophys Res Commun. 1994 Sep 15;203(2):1110-6.]</ref> | ||
|- | |- | ||
| Line 69: | Line 84: | ||
|mM | |mM | ||
|Human | |Human | ||
| − | |20mg/ml of 26 kDa protein | + | |Expression Vector: Human Cell |
| + | Enzyme: PGDS | ||
| + | pH: 8 | ||
| + | Temperature: 25 | ||
| + | |||
| + | Calculated: 20mg/ml of 26 kDa protein | ||
|<ref name="Zhou2010"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Zhou Y. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' FASEB J. 2010 Dec;24(12):4668-77.]</ref> | |<ref name="Zhou2010"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Zhou Y. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' FASEB J. 2010 Dec;24(12):4668-77.]</ref> | ||
|- | |- | ||
Revision as of 14:24, 28 May 2016
Contents
Reaction
Chemical equation
Rate equation
Parameters
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 4.00E-03 | 
|
Human | Expression Vector: Human Cell
Enzyme: PGDS pH: 8 Temperature: 25 |
[1] |
| 4.00E-03 |
|
Human | Expression Vector: Cerebrospinal Fluid
Enzyme: PGDS pH:10 Temperature: Unspecified |
[2] |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 158.4 | per minute | Human | Wild type (Paper includes 13 mutants of the enzyme) | [1] |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 0.000256 | mM | Human
Expression Vector: Cerebrospinal Fluid Enzyme: PGDS pH:10 Temperature: Unspecified Calculated: 20 ug/3ml of a 26 kDa protein. |
[2] | |
| 0.769 | mM | Human | Expression Vector: Human Cell
Enzyme: PGDS pH: 8 Temperature: 25 Calculated: 20mg/ml of 26 kDa protein |
[1] |
References
- ↑ 1.0 1.1 1.2 [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Zhou Y. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules. FASEB J. 2010 Dec;24(12):4668-77. doi: 10.1096/fj.10-164863. Epub 2010 Jul 28.
] Cite error: Invalid
<ref>tag; name "Zhou2010" defined multiple times with different content Cite error: Invalid<ref>tag; name "Zhou2010" defined multiple times with different content - ↑ 2.0 2.1 [http://www.ncbi.nlm.nih.gov/pubmed/8093029 Watanabe K. , "Identification of beta-trace as prostaglandin D synthase.
Biochem Biophys Res Commun. 1994 Sep 15;203(2):1110-6. ] Cite error: Invalid
<ref>tag; name "Watanabe1994" defined multiple times with different content
