Difference between revisions of "Transformation of PGH2 to PGD2"

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(Parameters)
(Parameters)
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| Wild type (Paper includes 13 mutants of the enzyme)
 
| Wild type (Paper includes 13 mutants of the enzyme)
 
| <ref name="Zhou2010"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Zhou Y. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' FASEB J. 2010 Dec;24(12):4668-77.]</ref>  
 
| <ref name="Zhou2010"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Zhou Y. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' FASEB J. 2010 Dec;24(12):4668-77.]</ref>  
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|}
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{|class="wikitable sortable"
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|+  style="text-align: left;" | Enzyme Concentrations
 +
|-
 +
! Value
 +
! Units
 +
! Species
 +
! Notes
 +
! Reference
 +
|-
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|0.000256
 +
|mM
 +
|Human
 +
|20 ug/3ml of a 26 kDa protein. Cerebral Fluid.
 +
|<ref name="Watanabe1994"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Watanabe K. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' Biochem Biophys Res Commun. 1994 Sep 15;203(2):1110-6.]</ref>
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|-
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|0.769
 +
|mM
 +
|Human
 +
|20mg/ml of 26 kDa protein
 +
|<ref name="Zhou2010"> [http://www.ncbi.nlm.nih.gov/pubmed/20667974 Zhou Y. , "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules.'' FASEB J. 2010 Dec;24(12):4668-77.]</ref>
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|-
 
|}
 
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Revision as of 16:50, 26 April 2016

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Reaction

R21 PGH2 - PGD2.jpg

Chemical equation

 FA \rightleftharpoons AA

Rate equation

Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
4.00E-03  mM Human Cerebrospinal Fluid [1]
4.00E-03  mM Human Human Wild Type Enzyme [2]
Enzyme Turnover Numbers
Value Units Species Notes Reference
158.4 per minute Human Wild type (Paper includes 13 mutants of the enzyme) [2]
Enzyme Concentrations
Value Units Species Notes Reference
0.000256 mM Human 20 ug/3ml of a 26 kDa protein. Cerebral Fluid. [1]
0.769 mM Human 20mg/ml of 26 kDa protein [2]

References

Related Reactions