Difference between revisions of "Transformation of LTC4 to exLTC4"
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! Species | ! Species | ||
! Notes | ! Notes | ||
+ | ! Weight | ||
! Reference | ! Reference | ||
|- | |- | ||
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|Substrate: LTC4 | |Substrate: LTC4 | ||
pH 7.0, 37°C, recombinant MRP2, using 4mM MgATP and 5g of isolated membranes. | pH 7.0, 37°C, recombinant MRP2, using 4mM MgATP and 5g of isolated membranes. | ||
+ | |512 | ||
|<ref name="Yas2007"> [http://ac.els-cdn.com/S0928098708003618/1-s2.0-S0928098708003618-main.pdf?_tid=a147bd90-1dc8-11e6-9405-00000aab0f26&acdnat=1463666001_5a08a971497da36ce8a604f84caadfc2 Yasunaga M. "Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2)'' Eur. J. Pharm. Sci. 35, 326-334 (2008)]</ref> | |<ref name="Yas2007"> [http://ac.els-cdn.com/S0928098708003618/1-s2.0-S0928098708003618-main.pdf?_tid=a147bd90-1dc8-11e6-9405-00000aab0f26&acdnat=1463666001_5a08a971497da36ce8a604f84caadfc2 Yasunaga M. "Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2)'' Eur. J. Pharm. Sci. 35, 326-334 (2008)]</ref> | ||
|- | |- | ||
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|Human | |Human | ||
|Substrate LTC4, 37 °C, 4 mM ATP, | |Substrate LTC4, 37 °C, 4 mM ATP, | ||
+ | |128 | ||
|<ref name="Mao2000"> [http://www.jbc.org/content/275/44/34166.full.pdf Zeng H. "Transport of amphipathic anions by human multidrug resistance protein 3'' J. Biol. Chem. 275, 34166-34172 (2000)]</ref> | |<ref name="Mao2000"> [http://www.jbc.org/content/275/44/34166.full.pdf Zeng H. "Transport of amphipathic anions by human multidrug resistance protein 3'' J. Biol. Chem. 275, 34166-34172 (2000)]</ref> | ||
|- | |- | ||
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|4 mM ATP, 37°C, 5–10 ml of membrane vesicle suspension (30 | |4 mM ATP, 37°C, 5–10 ml of membrane vesicle suspension (30 | ||
mg protein). | mg protein). | ||
+ | |128 | ||
|<ref name="Zeng2000"> [http://cancerres.aacrjournals.org/content/60/17/4779.full.pdf "Functional reconstitution of substrate transport by purified multidrug resistance protein MRP1 (ABCC1) in phospholipid vesicles'' Cancer Res. 60, 4779-4784 (2000)]</ref> | |<ref name="Zeng2000"> [http://cancerres.aacrjournals.org/content/60/17/4779.full.pdf "Functional reconstitution of substrate transport by purified multidrug resistance protein MRP1 (ABCC1) in phospholipid vesicles'' Cancer Res. 60, 4779-4784 (2000)]</ref> | ||
|- | |- | ||
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|pH 7.0, 37°C, recombinant MRP2, | |pH 7.0, 37°C, recombinant MRP2, | ||
Substrate: 17beta-estradiol 17-(beta-D-glucuronide) | Substrate: 17beta-estradiol 17-(beta-D-glucuronide) | ||
+ | |512 | ||
|<ref name="Yas2007"> [http://ac.els-cdn.com/S0928098708003618/1-s2.0-S0928098708003618-main.pdf?_tid=a147bd90-1dc8-11e6-9405-00000aab0f26&acdnat=1463666001_5a08a971497da36ce8a604f84caadfc2 Yasunaga M. "Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2)'' Eur. J. Pharm. Sci. 35, 326-334 (2008)]</ref> | |<ref name="Yas2007"> [http://ac.els-cdn.com/S0928098708003618/1-s2.0-S0928098708003618-main.pdf?_tid=a147bd90-1dc8-11e6-9405-00000aab0f26&acdnat=1463666001_5a08a971497da36ce8a604f84caadfc2 Yasunaga M. "Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2)'' Eur. J. Pharm. Sci. 35, 326-334 (2008)]</ref> | ||
|- | |- | ||
Line 50: | Line 55: | ||
|pH 7.0, 37°C, recombinant MRP2, | |pH 7.0, 37°C, recombinant MRP2, | ||
Substrate: Estrone glucuronide | Substrate: Estrone glucuronide | ||
+ | |512 | ||
|<ref name="Yas2007"> [http://ac.els-cdn.com/S0928098708003618/1-s2.0-S0928098708003618-main.pdf?_tid=a147bd90-1dc8-11e6-9405-00000aab0f26&acdnat=1463666001_5a08a971497da36ce8a604f84caadfc2 Yasunaga M. "Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2)'' Eur. J. Pharm. Sci. 35, 326-334 (2008)]</ref> | |<ref name="Yas2007"> [http://ac.els-cdn.com/S0928098708003618/1-s2.0-S0928098708003618-main.pdf?_tid=a147bd90-1dc8-11e6-9405-00000aab0f26&acdnat=1463666001_5a08a971497da36ce8a604f84caadfc2 Yasunaga M. "Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2)'' Eur. J. Pharm. Sci. 35, 326-334 (2008)]</ref> | ||
|- | |- | ||
Line 56: | Line 62: | ||
|Human | |Human | ||
|pH 7.4, 37°C, Substrate: Progesterone, Expression vector: Human HepG2 hepatoma | |pH 7.4, 37°C, Substrate: Progesterone, Expression vector: Human HepG2 hepatoma | ||
+ | |2048 | ||
|<ref name="Fong2007"> [https://www.thieme-connect.com/products/ejournals/html/10.1055/s-2007-967120 W. Fong "Schisandrol A from Schisandra chinensis Reverses P-Glycoprotein-Mediated Multidrug Resistance by Affecting Pgp-Substrate Complexes'' Planta Med 2007; 73(3): 212-220]</ref> | |<ref name="Fong2007"> [https://www.thieme-connect.com/products/ejournals/html/10.1055/s-2007-967120 W. Fong "Schisandrol A from Schisandra chinensis Reverses P-Glycoprotein-Mediated Multidrug Resistance by Affecting Pgp-Substrate Complexes'' Planta Med 2007; 73(3): 212-220]</ref> | ||
|- | |- | ||
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! Transporter | ! Transporter | ||
! Notes | ! Notes | ||
+ | ! Weight | ||
! Reference | ! Reference | ||
|- | |- | ||
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|Organism: Bacteria Escherichia coli | |Organism: Bacteria Escherichia coli | ||
Back of the envelope calculation by BioNumbers: http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=102931 | Back of the envelope calculation by BioNumbers: http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=102931 | ||
+ | |8 | ||
|<ref name="Waygood1980"> [https://www.ncbi.nlm.nih.gov/pubmed/6992959?dopt=Abstract Waygood EB ''Enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli. Purification to homogeneity and some properties.''Can J Biochem. 1980 Jan;58(1):40-8.]</ref> | |<ref name="Waygood1980"> [https://www.ncbi.nlm.nih.gov/pubmed/6992959?dopt=Abstract Waygood EB ''Enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli. Purification to homogeneity and some properties.''Can J Biochem. 1980 Jan;58(1):40-8.]</ref> | ||
|- | |- | ||
Line 92: | Line 101: | ||
|Organism: Bacteria Escherichia coli | |Organism: Bacteria Escherichia coli | ||
Back of the envelop calculation on bionumbers http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=103159 | Back of the envelop calculation on bionumbers http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=103159 | ||
+ | |8 | ||
|<ref name="Naftalin2007"> [https://www.ncbi.nlm.nih.gov/pubmed/17325012?dopt=Abstract Naftalin R. J. ''ELactose permease H+-lactose symporter: mechanical switch or Brownian ratchet?''Biophys J. 2007 May 15;92(10):3474-91. Epub 2007 Feb 26.]</ref> | |<ref name="Naftalin2007"> [https://www.ncbi.nlm.nih.gov/pubmed/17325012?dopt=Abstract Naftalin R. J. ''ELactose permease H+-lactose symporter: mechanical switch or Brownian ratchet?''Biophys J. 2007 May 15;92(10):3474-91. Epub 2007 Feb 26.]</ref> | ||
|- | |- | ||
Line 99: | Line 109: | ||
|Organism: Budding yeast Saccharomyces cerevisiae | |Organism: Budding yeast Saccharomyces cerevisiae | ||
Back of the envelope calculation http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=101737 | Back of the envelope calculation http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=101737 | ||
+ | |8 | ||
|<ref name="Ye2001"> [https://www.ncbi.nlm.nih.gov/pubmed/11561293?dopt=Abstract Ye L. ''Expression and activity of the Hxt7 high-affinity hexose transporter of Saccharomyces cerevisiae''Yeast. 2001 Sep 30;18(13):1257-67.]</ref> | |<ref name="Ye2001"> [https://www.ncbi.nlm.nih.gov/pubmed/11561293?dopt=Abstract Ye L. ''Expression and activity of the Hxt7 high-affinity hexose transporter of Saccharomyces cerevisiae''Yeast. 2001 Sep 30;18(13):1257-67.]</ref> | ||
|- | |- | ||
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|pH 7.0, 37°C, recombinant MRP2, | |pH 7.0, 37°C, recombinant MRP2, | ||
Substrate: Estrone glucuronide | Substrate: Estrone glucuronide | ||
+ | |8 | ||
|<ref name="Yas2007"> [http://ac.els-cdn.com/S0928098708003618/1-s2.0-S0928098708003618-main.pdf?_tid=a147bd90-1dc8-11e6-9405-00000aab0f26&acdnat=1463666001_5a08a971497da36ce8a604f84caadfc2 Yasunaga M. "Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2)'' Eur. J. Pharm. Sci. 35, 326-334 (2008)]</ref> | |<ref name="Yas2007"> [http://ac.els-cdn.com/S0928098708003618/1-s2.0-S0928098708003618-main.pdf?_tid=a147bd90-1dc8-11e6-9405-00000aab0f26&acdnat=1463666001_5a08a971497da36ce8a604f84caadfc2 Yasunaga M. "Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2)'' Eur. J. Pharm. Sci. 35, 326-334 (2008)]</ref> | ||
|- | |- | ||
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! Species | ! Species | ||
! Notes | ! Notes | ||
+ | ! Weight | ||
! Reference | ! Reference | ||
|- | |- | ||
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pH: 7.5 | pH: 7.5 | ||
Temperature: 37 °C | Temperature: 37 °C | ||
+ | |2048 | ||
|<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref> | |<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the human proteome'' Nature, 2014 509, 582–587]</ref> | ||
|- | |- | ||
Line 177: | Line 191: | ||
pH: 7.5 | pH: 7.5 | ||
Temperature: 37 °C | Temperature: 37 °C | ||
+ | |2048 | ||
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref> | |<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref> | ||
|- | |- | ||
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pH: 7.5 | pH: 7.5 | ||
Temperature: 37 °C | Temperature: 37 °C | ||
+ | |2048 | ||
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref> | |<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref> | ||
|- | |- |
Revision as of 14:20, 22 May 2019
Return to ATP-Binding Cassette Transporters
Contents
Rate equation
Transport Parameters
Kms
Value | Units | Species | Notes | Weight | Reference |
---|---|---|---|---|---|
0.0109 ± 0.0041 | mM | Human | Substrate: LTC4
pH 7.0, 37°C, recombinant MRP2, using 4mM MgATP and 5g of isolated membranes. |
512 | [1] |
0.0000366 ± 0.0000038 | mM | Human | Substrate LTC4, 37 °C, 4 mM ATP, | 128 | [2] |
5.3E-3 ± 2.6E-3 | mM | Human | 4 mM ATP, 37°C, 5–10 ml of membrane vesicle suspension (30
mg protein). |
128 | [3] |
0.1954 ± 0.0612 | mM | Human | pH 7.0, 37°C, recombinant MRP2,
Substrate: 17beta-estradiol 17-(beta-D-glucuronide) |
512 | [1] |
0.1097 ± 0.0391 | mM | Human | pH 7.0, 37°C, recombinant MRP2,
Substrate: Estrone glucuronide |
512 | [1] |
0.0207 | mM | Human | pH 7.4, 37°C, Substrate: Progesterone, Expression vector: Human HepG2 hepatoma | 2048 | [4] |
Mode (mM) | Confidence Interval | Location parameter (µ) | Scale parameter (σ) |
---|---|---|---|
1.13E+01 | 1.00E+01 | 3.22E+00 | 8.91E-01 |
kcat
No data available on the turnover of ABCC4 transporter therefore based upon estimates made by http://book.bionumbers.org/what-are-the-rates-of-membrane-transporters/
Value | Units | Transporter | Notes | Weight | Reference |
---|---|---|---|---|---|
3.5 | min-1 | Glucose transporter ptsI | Organism: Bacteria Escherichia coli
Back of the envelope calculation by BioNumbers: http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=102931 |
8 | [5] |
0.33 - 0.83 | min-1 | Rate of transport of H+/Lactose transporter | Organism: Bacteria Escherichia coli
Back of the envelop calculation on bionumbers http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=103159 |
8 | [6] |
3.28 | min-1 | Catalytic Rate of transporter HXT7 | Organism: Budding yeast Saccharomyces cerevisiae
Back of the envelope calculation http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=101737 |
8 | [7] |
11.34 | min-1 | Human | pH 7.0, 37°C, recombinant MRP2,
Substrate: Estrone glucuronide |
8 | [1] |
Mode (min-1) | Confidence Interval | Location parameter (µ) | Scale parameter (σ) |
---|---|---|---|
2.10E-02 | 2.37E+01 | -2.60E+00 | 1.12E+00 |
Vmax
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
0.00019 ± 1.96e-5 | mmol/min/mg protein | Human | Substrate: LTC4
pH 7.0, 37°C, recombinant MRP2, using 4mM MgATP and 5g of isolated membranes. |
[1] |
1.25e-7 ± 1.2e-8 | mmol/min/mg | Human | Substrate LTC4, 37 °C, 4 mM ATP, | [2] |
2.02e-8 ± 5.9e-9 | mmol/mg/min | Human | 4 mM ATP, 37°C, 5–10 ml of membrane vesicle suspension (30
mg protein). |
[3] |
Transporter concentration
Value | Units | Species | Notes | Weight | Reference |
---|---|---|---|---|---|
5.15 | ppm | Human | Expression Vector: Stomach
Enzyme: ABCC4 pH: 7.5 Temperature: 37 °C |
2048 | [8] |
5.94 | ppm | Human | Expression Vector: Lung
Enzyme: ABCC4 pH: 7.5 Temperature: 37 °C |
2048 | [9] |
2.66 | ppm | Human | Expression Vector: Gut
Enzyme: ABCC4 pH: 7.5 Temperature: 37 °C |
2048 | [9] |
Mode (mM) | Confidence Interval | Location parameter (µ) | Scale parameter (σ) |
---|---|---|---|
5.06E+00 | 1.44E+00 | 1.74E+00 | 3.42E-01 |
References
- ↑ 1.0 1.1 1.2 1.3 1.4 Yasunaga M. "Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2) Eur. J. Pharm. Sci. 35, 326-334 (2008)
- ↑ 2.0 2.1 Zeng H. "Transport of amphipathic anions by human multidrug resistance protein 3 J. Biol. Chem. 275, 34166-34172 (2000) Cite error: Invalid
<ref>
tag; name "Mao2000" defined multiple times with different content - ↑ 3.0 3.1 "Functional reconstitution of substrate transport by purified multidrug resistance protein MRP1 (ABCC1) in phospholipid vesicles Cancer Res. 60, 4779-4784 (2000)
- ↑ W. Fong "Schisandrol A from Schisandra chinensis Reverses P-Glycoprotein-Mediated Multidrug Resistance by Affecting Pgp-Substrate Complexes Planta Med 2007; 73(3): 212-220
- ↑ Waygood EB Enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli. Purification to homogeneity and some properties.Can J Biochem. 1980 Jan;58(1):40-8.
- ↑ Naftalin R. J. ELactose permease H+-lactose symporter: mechanical switch or Brownian ratchet?Biophys J. 2007 May 15;92(10):3474-91. Epub 2007 Feb 26.
- ↑ Ye L. Expression and activity of the Hxt7 high-affinity hexose transporter of Saccharomyces cerevisiaeYeast. 2001 Sep 30;18(13):1257-67.
- ↑ M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587
- ↑ 9.0 9.1 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581