Difference between revisions of "Transformation of LTA4 to LTC4"

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[[Welcome to the In-Silico Model of Cutaneous Lipids Wiki | Return to overview]]
 
[[Welcome to the In-Silico Model of Cutaneous Lipids Wiki | Return to overview]]
  
−
To generate LTC4, a supramolecular complex of 5-LOX, FLAP and leukotriene C4 synthase is formed on the nuclear membrane due to the increase of intracellular calcium \cite{Woods1993, Hammarberg2000, Radmark2015, Evans2008, Mandal2004, Mandal2008}
+
Leukotriene C4 synthase (LTC4S) transforms LTA4 into LTC4. This reaction involves the conjugation of the LTA4 epoxide and glutathione.
  
 
== Reaction ==
 
== Reaction ==
  
−
 
+
[[File:R16reaction.png|center|500px]]
  
 
==Chemical equation==
 
==Chemical equation==
  
−
<center><math> AA \rightleftharpoons PGH2 </math></center>
+
<center><math> LTA4 \rightleftharpoons LTC4 </math></center>
  
 
== Rate equation ==
 
== Rate equation ==
  
 +
[[File:R16.PNG|center|500px]]
  
 
== Parameters ==
 
== Parameters ==
−
 
+
===K<sub>ms</sub>===
 
{|class="wikitable sortable"  
 
{|class="wikitable sortable"  
−
|+  style="text-align: left;" | Michaelis-Menten Constants
+
|+  style="text-align: left;" | Literature Values
 
|-
 
|-
 
! Value
 
! Value
Line 23: Line 24:
 
! Species
 
! Species
 
! Notes
 
! Notes
 +
! Weight
 
! Reference
 
! Reference
 
|-
 
|-
−
|0.3 �± 0.06
+
|0.3 ± 0.06
 
|mM
 
|mM
 
|Human
 
|Human
Line 32: Line 34:
 
pH:7.8
 
pH:7.8
 
Temperature:20 °C
 
Temperature:20 °C
 +
|256
 
|<ref name="Rinaldo2010"> [http://www.jbc.org/content/285/52/40771.full.pdf  Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4
 
|<ref name="Rinaldo2010"> [http://www.jbc.org/content/285/52/40771.full.pdf  Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4
 
Synthase'' J Biochem 2010, 285, 40771-40776]</ref>
 
Synthase'' J Biochem 2010, 285, 40771-40776]</ref>
Line 42: Line 45:
 
pH: 7.8
 
pH: 7.8
 
Temperature: 37 °C
 
Temperature: 37 °C
−
|<ref name="Niegowski2013"> [http://www.jbc.org/content/early/2013/12/23/jbc.M113.534628 Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the
+
|1024
−
enzyme mechanism'' J. Biol. Chem. 289, 5199-5207 (2014)]</ref>
+
|<ref name="Niegowski2013"> [http://www.jbc.org/content/early/2013/12/23/jbc.M113.534628 Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the enzyme mechanism'' J. Biol. Chem. 289, 5199-5207 (2014)]</ref>
 +
|-
 +
|}
 +
 
 +
{| class="wikitable"
 +
|+  style="text-align: left;" | Description of the LTC4S Kms distribution
 +
! Mode (mM) !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ)
 
|-
 
|-
 +
| 3.15E-02 || 7.18E+00 || -2.83E+00 || 7.90E-01
 
|}
 
|}
  
 +
[[Image:53.jpg|none|thumb|500px|The estimated probability distribution for LTC4S Kms. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.  ]]
 +
 +
===K<sub>mp</sub>===
 +
{| class="wikitable"
 +
|+  style="text-align: left;" | Description of the LTC4S Kmp distribution
 +
! Mode (mM) !! Location parameter (µ) !! Scale parameter (σ)
 +
|-
 +
| 3.18E-02 || -2.82E+00 || 7.92E-01
 +
|}
 +
 +
[[Image:54.jpg|none|thumb|500px|The estimated probability distribution for LTC4S Kmp. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.  ]]
 +
 +
===k<sub>cat</sub>===
 
{|class="wikitable sortable"  
 
{|class="wikitable sortable"  
−
|+  style="text-align: left;" | Enzyme Turnover Numbers
+
|+  style="text-align: left;" | Literature Values
 
|-
 
|-
 
! Value
 
! Value
Line 54: Line 77:
 
! Species
 
! Species
 
! Notes
 
! Notes
 +
! Weight
 
! Reference
 
! Reference
 
|-
 
|-
Line 63: Line 87:
 
pH:7.8
 
pH:7.8
 
Temperature:20 °C
 
Temperature:20 °C
 +
|256
 
|<ref name="Rinaldo2010"> [http://www.jbc.org/content/285/52/40771.full.pdf  Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4
 
|<ref name="Rinaldo2010"> [http://www.jbc.org/content/285/52/40771.full.pdf  Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4
 
Synthase'' J Biochem 2010, 285, 40771-40776]</ref>
 
Synthase'' J Biochem 2010, 285, 40771-40776]</ref>
Line 73: Line 98:
 
pH: 7.8
 
pH: 7.8
 
Temperature: 37 °C
 
Temperature: 37 °C
−
|<ref name="Niegowski2013"> [http://www.jbc.org/content/289/8/5199.full Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the
+
|1024
−
enzyme mechanism'' J. Biol. Chem. 289, 5199-5207 (2014)]</ref>
+
|<ref name="Niegowski2013"> [http://www.jbc.org/content/early/2013/12/23/jbc.M113.534628 Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the enzyme mechanism'' J. Biol. Chem. 289, 5199-5207 (2014)]</ref>
 +
|-
 +
|}
 +
 
 +
{| class="wikitable"
 +
|+  style="text-align: left;" | Description of the LTC4S kcat distribution
 +
! Mode (min-1) !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ)
 
|-
 
|-
 +
| 1.47E+03 || 1.42E+00 || 7.40E+00 || 3.29E-01
 
|}
 
|}
 +
 +
[[Image:55.jpg|none|thumb|500px|The estimated probability distribution for LTC4S kcat. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.  ]]
 +
 +
=== Enzyme concentration ===
 +
 +
To convert the enzyme concentration from ppm to mM, the following [[Common equations#Enzyme concentration (mM)|equation]] was used.
  
 
{|class="wikitable sortable"  
 
{|class="wikitable sortable"  
−
|+  style="text-align: left;" | LTC4S Abundance
+
|+  style="text-align: left;" | Literature Values
 
|-
 
|-
 
! Value
 
! Value
Line 85: Line 123:
 
! Species
 
! Species
 
! Notes
 
! Notes
 +
! Weight
 
! Reference
 
! Reference
 
|-
 
|-
Line 94: Line 133:
 
pH: 7.5
 
pH: 7.5
 
Temperature: 37 °C
 
Temperature: 37 °C
 +
|1024
 
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 
|-
 
|-
Line 103: Line 143:
 
pH: 7.5
 
pH: 7.5
 
Temperature: 37 °C
 
Temperature: 37 °C
 +
|2048
 
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 
|-
 
|-
Line 112: Line 153:
 
pH: 7.5
 
pH: 7.5
 
Temperature: 37 °C
 
Temperature: 37 °C
 +
|1024
 
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 
|-
 
|-
 
|}
 
|}
  
 +
 +
{| class="wikitable"
 +
|+  style="text-align: left;" | Description of the LTC4S concentration distribution
 +
! Mode (mM) !! Mode (ppm) !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ)
 +
|-
 +
| 2.90E+01 ||1.60E-04|| 1.44E+00 || 3.49E+00 || 3.46E-01
 +
|-
 +
|}
 +
 +
[[Image:160.jpg|none|thumb|500px|The estimated probability distribution for LTC4S concentration. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.  ]]
 +
 +
=== K<sub>eq</sub>===
 
{|class="wikitable sortable"  
 
{|class="wikitable sortable"  
−
|+  style="text-align: left;" | Gibbs Free Energy Change
+
|+  style="text-align: left;" | Literature Values
 
|-
 
|-
−
! Value
+
! Gibbs Free Energy Change
 
! Units
 
! Units
 
! Species
 
! Species
 
! Notes
 
! Notes
 +
! Weight
 
! Reference
 
! Reference
 
|-
 
|-
Line 134: Line 189:
 
pH: 7.3  
 
pH: 7.3  
 
ionic strength: 0.25
 
ionic strength: 0.25
 +
|64
 
|<ref name="MetaCyc”>[http://metacyc.org/META/NEW-IMAGE?type=REACTION&object=RXN66-490 Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471]</ref>
 
|<ref name="MetaCyc”>[http://metacyc.org/META/NEW-IMAGE?type=REACTION&object=RXN66-490 Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471]</ref>
 
|}
 
|}
  
−
==References==
+
{| class="wikitable"
−
<references/>
+
|+  style="text-align: left;" | Description of the LTC4S Keq distribution
 +
! Mode (mM) !! Confidence Interval !! Location parameter (µ) !! Scale parameter (σ)
 +
|-
 +
| 5.13E-08 || 1.00E+01 || -1.60E+01 || 8.90E-01
 +
|}
 +
 
 +
[[Image:56.jpg|none|thumb|500px|The estimated probability distribution for LTC4S Keq. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.  ]]
  
 
== Related Reactions ==
 
== Related Reactions ==
  
−
 
+
==References==
−
{| class="wikitable"
+
<references/>
−
! style="text-align: center; font-weight: bold;" | Reaction #
 
−
! style="text-align: center; font-weight: bold;" | Species
 
−
! style="text-align: center; font-weight: bold;" | Half Life
 
−
  (min)
 
−
! style="text-align: center; font-weight: bold;" | Rate constant
 
−
  (min -1)
 
−
! style="text-align: center; font-weight: bold;" | Notes
 
−
! style="text-align: center; font-weight: bold;" | Reference
 
−
|-
 
−
| style="text-align: center;" | 44
 
−
| style="text-align: center;" | exPGF2a
 
−
| style="text-align: center;" | 528 ±  204
 
−
| style="text-align: center;" | 0.001 ± 0.003
 
−
| style="text-align: center;" |Study performed in decidual stromal cells and macrophages in culture.
 
−
| style="text-align: center;" | <ref name="Ishihara1991”>[http://www.ncbi.nlm.nih.gov/pubmed/1789996 O. Ishihara, "Differences of metabolism of prostaglandin E2 and F2 alpha by decidual stromal cells and macrophages in culture." Eicosanoids. 1991;4(4):203-7.]</ref>
 
−
|-
 
−
| style="text-align: center;" | 45
 
−
| style="text-align: center;" | exTXB2
 
−
| style="text-align: center;" | 20 to 30
 
−
| style="text-align: center;" | 0.035 to 0.023
 
−
| style="text-align: center;" |Quoted in a textbook(https://books.google.co.uk/books?id=_9kEeTjyJdMC&pg=PA864&lpg=PA864&dq=half+life+txa2&source=bl&ots=2OTF4Mh2Jk&sig=hu79GprliUcW4QE_Zm79islesOA&hl=en&sa=X&ved=0ahUKEwj0oo2sgfjOAhXLIcAKHcaPDHQQ6AEIRjAI#v=onepage&q=half%20life%20txa2&f=false) with no ref.
 
−
| style="text-align: center;" |
 
−
|-
 
−
| style="text-align: center;" | 46
 
−
| style="text-align: center;" | exTXA2
 
−
| style="text-align: center;" | 0.333
 
−
| style="text-align: center;" | 2.079
 
−
| style="text-align: center;" |Quoted in a textbook(https://books.google.co.uk/books?id=_9kEeTjyJdMC&pg=PA864&lpg=PA864&dq=half+life+txa2&source=bl&ots=2OTF4Mh2Jk&sig=hu79GprliUcW4QE_Zm79islesOA&hl=en&sa=X&ved=0ahUKEwj0oo2sgfjOAhXLIcAKHcaPDHQQ6AEIRjAI#v=onepage&q=half%20life%20txa2&f=false) with no ref.
 
−
| style="text-align: center;" |
 
−
|-
 
−
| style="text-align: center;" | 47
 
−
| style="text-align: center;" | ex6-KETO-PGF2A
 
−
| style="text-align: center;" | 3
 
−
| style="text-align: center;" | 0.231
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |
 
−
|-
 
−
| style="text-align: center;" | 48
 
−
| style="text-align: center;" | exPGI2
 
−
| style="text-align: center;" | 0.7
 
−
| style="text-align: center;" | 0.990
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |<ref name="Cawello1994”>[https://www.ncbi.nlm.nih.gov/pubmed/8070511 Cawello W., "Metabolism and pharmacokinetics of prostaglandin E1 administered by intravenous infusion in human subjects." Eur J Clin Pharmacol. 1994;46(3):275-7.]</ref>
 
−
|-
 
−
| style="text-align: center;" | 49
 
−
| style="text-align: center;" | exPGE2
 
−
| style="text-align: center;" | 900,± 492
 
−
| style="text-align: center;" | 0.001 ± 0.001
 
−
| style="text-align: center;" | Study performed in decidual stromal cells and macrophages in culture.
 
−
| style="text-align: center;" | <ref name="Ishihara1991”>[http://www.ncbi.nlm.nih.gov/pubmed/1789996 O. Ishihara, "Differences of metabolism of prostaglandin E2 and F2 alpha by decidual stromal cells and macrophages in culture." Eicosanoids. 1991;4(4):203-7.]</ref>
 
−
|-
 
−
| style="text-align: center;" | 50
 
−
| style="text-align: center;" | ex15-DEOXY-PGJ2
 
−
| style="text-align: center;" | 720
 
−
| style="text-align: center;" | 0.001
 
−
| style="text-align: center;" | Dehydration of PGD2 to ultimatley 15d-PGJ2 occurs with a half life of about 12 hours in the presense of albumin (protien found in blood).
 
−
| style="text-align: center;" | <ref name="Fitzpatrick1983”>[http://www.ncbi.nlm.nih.gov/pubmed/6578214 F. Fitzpatrick, "Albumin-catalyzed metabolism of prostaglandin D2. Identification of products formed in vitro." J Biol Chem. 1983 Oct 10;258(19):11713-8.]</ref>
 
−
|-
 
−
| style="text-align: center;" | 51
 
−
| style="text-align: center;" | exPGJ2
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |
 
−
|-
 
−
| style="text-align: center;" | 52
 
−
| style="text-align: center;" | exPGD2
 
−
| style="text-align: center;" | 1.5 - 1.6
 
−
| style="text-align: center;" | 0.462 to 0.433
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |
 
−
|-
 
−
| style="text-align: center;" | 53
 
−
| style="text-align: center;" | exPGH2
 
−
| style="text-align: center;" | 5
 
−
| style="text-align: center;" | 0.139
 
−
| style="text-align: center;" | Quoted on supplier page (http://www.enzolifesciences.com/BML-PH002/prostaglandin-h2/)
 
−
| style="text-align: center;" |
 
−
|-
 
−
| style="text-align: center;" | 54
 
−
| style="text-align: center;" | ex5-OXO-ETE
 
−
| style="text-align: center;" | 11
 
−
| style="text-align: center;" | 0.064
 
−
| style="text-align: center;" | Study in R15L Cells
 
−
| style="text-align: center;" | <ref name="Cong2009”>[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2730384/ Cong W., "15-oxo-Eicosatetraenoic Acid, a Metabolite of Macrophage 15-Hydroxyprostaglandin Dehydrogenase That Inhibits Endothelial Cell Proliferation" Mol Pharmacol. 2009 Sep; 76(3): 516–525.]</ref>
 
−
|-
 
−
| style="text-align: center;" | 55
 
−
| style="text-align: center;" | ex5-HETE
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |
 
−
|-
 
−
| style="text-align: center;" | 56
 
−
| style="text-align: center;" | exLTB4
 
−
| style="text-align: center;" | 0.47 ± 0.02 to 0.63 ± 0.04
 
−
| style="text-align: center;" | 1.475 ± 34.657 to 1.100 ± 17.329
 
−
| style="text-align: center;" | Rabbit, Immunoreactive LTB4
 
−
| style="text-align: center;" | <ref name="Marleau1994”>[http://www.ncbi.nlm.nih.gov/pubmed/8075884 Marleau S., "Metabolic disposition of leukotriene B4 (LTB4) and oxidation-resistant analogues of LTB4 in conscious rabbits." Br J Pharmacol. 1994 Jun;112(2):654-8.]</ref>
 
−
|-
 
−
| style="text-align: center;" | 57
 
−
| style="text-align: center;" | exLTC4
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |
 
−
|-
 
−
| style="text-align: center;" | 58
 
−
| style="text-align: center;" | exLTA4
 
−
| style="text-align: center;" | 0.05
 
−
| style="text-align: center;" | 13.863
 
−
| style="text-align: center;" | 37 degrees C
 
−
| style="text-align: center;" | <ref name="Zimmer2004”>[http://www.jlr.org/content/45/11/2138.long Zimmer J., "Fatty acid binding proteins stabilize leukotriene A4 competition with arachidonic acid but not other lipoxygenase products" November 2004 The Journal of Lipid Research, 45, 2138-2144.]</ref>
 
−
|-
 
−
| style="text-align: center;" | 59
 
−
| style="text-align: center;" | ex5-HPETE
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |
 
−
|-
 
−
| style="text-align: center;" | 60
 
−
| style="text-align: center;" | ex15-HETE
 
−
| style="text-align: center;" | 21
 
−
| style="text-align: center;" | 0.0331
 
−
| style="text-align: center;" | Study in R15L Cells
 
−
| style="text-align: center;" | <ref name="Cong2009”>[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2730384/ Cong W., "15-oxo-Eicosatetraenoic Acid, a Metabolite of Macrophage 15-Hydroxyprostaglandin Dehydrogenase That Inhibits Endothelial Cell Proliferation" Mol Pharmacol. 2009 Sep; 76(3): 516–525.]</ref>
 
−
|-
 
−
| style="text-align: center;" | 61
 
−
| style="text-align: center;" | ex15-HPETE
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |
 
−
|-
 
−
| style="text-align: center;" | 62
 
−
| style="text-align: center;" | ex12-HETE
 
−
| style="text-align: center;" | 180
 
−
| style="text-align: center;" | 0.004
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |
 
−
|-
 
−
| style="text-align: center;" | 63
 
−
| style="text-align: center;" | ex12-HPETE
 
−
| style="text-align: center;" | 0.5
 
−
| style="text-align: center;" | 1.386
 
−
| style="text-align: center;" | "During the first 2 min., the half-life of 12-HETE was 0.9 s, which implies
 
−
a fast clearance of the compound from the circulation. However, during
 
−
the subsequent half-hour the estimated half-life was 3 min. and increased
 
−
dramatically at the interval of time from 30 to 60 min. (t1/2 around 3 h)."
 
−
| style="text-align: center;" | <ref name="Dadaian1998”>[http://www.ncbi.nlm.nih.gov/pubmed/9661215 Dadaian  M., "12-hydroxyeicosatetraenoic acid is a long-lived substance in the rabbit circulation." Prostaglandins Other Lipid Mediat. 1998 Jan;55(1):3-25.]</ref>
 
−
|-
 
−
| style="text-align: center;" | 64
 
−
| style="text-align: center;" | exAA
 
−
| style="text-align: center;" | 240 to 660
 
−
| style="text-align: center;" | 0.003 to 0.001
 
−
| style="text-align: center;" |
 
−
| style="text-align: center;" |<ref name="Vinge1985”>[http://www.ncbi.nlm.nih.gov/pubmed/3921386 Vinge E., "Arachidonic acid-induced platelet aggregation and prostanoid formation in whole blood in relation to plasma concentration of indomethacin." Eur J Clin Pharmacol. 1985;28(2):163-9.]</ref>
 
−
|}
 

Latest revision as of 11:12, 2 November 2019

Return to overview

Leukotriene C4 synthase (LTC4S) transforms LTA4 into LTC4. This reaction involves the conjugation of the LTA4 epoxide and glutathione.

Reaction

R16reaction.png

Chemical equation

LTA4 \rightleftharpoons LTC4

Rate equation

R16.PNG

Parameters

Kms

Literature Values
Value Units Species Notes Weight Reference
0.3 ± 0.06 mM Human Expression Vector: E. Coli.

Enzyme: Wild Type hLTC4S pH:7.8 Temperature:20 °C

256 [1]
3.00E-02 ± 1.00E-02 mM Human Expression Vector: E Coli

Enzyme: Wild type LTC4S pH: 7.8 Temperature: 37 °C

1024 [2]
Description of the LTC4S Kms distribution
Mode (mM) Confidence Interval Location parameter (µ) Scale parameter (σ)
3.15E-02 7.18E+00 -2.83E+00 7.90E-01
The estimated probability distribution for LTC4S Kms. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

Kmp

Description of the LTC4S Kmp distribution
Mode (mM) Location parameter (µ) Scale parameter (σ)
3.18E-02 -2.82E+00 7.92E-01
The estimated probability distribution for LTC4S Kmp. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

kcat

Literature Values
Value Units Species Notes Weight Reference
702 per minute Human Expression Vector: E. Coli.

Enzyme: Wild Type hLTC4S pH:7.8 Temperature:20 °C

256 [1]
1560 ± 240 per minute Human Expression Vector: E Coli

Enzyme: Wild type LTC4S pH: 7.8 Temperature: 37 °C

1024 [2]
Description of the LTC4S kcat distribution
Mode (min-1) Confidence Interval Location parameter (µ) Scale parameter (σ)
1.47E+03 1.42E+00 7.40E+00 3.29E-01
The estimated probability distribution for LTC4S kcat. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

Enzyme concentration

To convert the enzyme concentration from ppm to mM, the following equation was used.

Literature Values
Value Units Species Notes Weight Reference
26.8 ppm Human Expression Vector: Lung

Enzyme: LTC4S pH: 7.5 Temperature: 37 °C

1024 [3]
33.0 ppm Human Expression Vector: Esophagus

Enzyme: LTC4S pH: 7.5 Temperature: 37 °C

2048 [3]
13.9 ppm Human Expression Vector: Adrenal Gland

Enzyme: LTC4S pH: 7.5 Temperature: 37 °C

1024 [3]


Description of the LTC4S concentration distribution
Mode (mM) Mode (ppm) Confidence Interval Location parameter (µ) Scale parameter (σ)
2.90E+01 1.60E-04 1.44E+00 3.49E+00 3.46E-01
The estimated probability distribution for LTC4S concentration. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

Keq

Literature Values
Gibbs Free Energy Change Units Species Notes Weight Reference
9.934128 kcal/mol Not stated Estimated

Enzyme: LTC4S Substrate: LTA4 Product: LTC4 pH: 7.3 ionic strength: 0.25

64 [4]
Description of the LTC4S Keq distribution
Mode (mM) Confidence Interval Location parameter (µ) Scale parameter (σ)
5.13E-08 1.00E+01 -1.60E+01 8.90E-01
The estimated probability distribution for LTC4S Keq. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

Related Reactions

References

  1. ↑ 1.0 1.1 [http://www.jbc.org/content/285/52/40771.full.pdf Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4 Synthase J Biochem 2010, 285, 40771-40776]
  2. ↑ 2.0 2.1 Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the enzyme mechanism J. Biol. Chem. 289, 5199-5207 (2014)
  3. ↑ 3.0 3.1 3.2 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
  4. ↑ Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471
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