Difference between revisions of "Transformation of LTA4 to LTC4"
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| 3.15E-02 || 7.18E+00 || -2.83E+00 || 7.90E-01 | | 3.15E-02 || 7.18E+00 || -2.83E+00 || 7.90E-01 | ||
|} | |} | ||
| + | |||
| + | [[Image:53.jpg|none|thumb|500px|The estimated probability distribution for LTC4S Kms. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale. ]] | ||
===K<sub>mp</sub>=== | ===K<sub>mp</sub>=== | ||
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|} | |} | ||
| − | + | [[Image:54.jpg|none|thumb|500px|The estimated probability distribution for LTC4S Kmp. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale. ]] | |
===k<sub>cat</sub>=== | ===k<sub>cat</sub>=== | ||
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|} | |} | ||
| − | + | [[Image:55.jpg|none|thumb|500px|The estimated probability distribution for LTC4S kcat. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale. ]] | |
=== Enzyme concentration === | === Enzyme concentration === | ||
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| | | | ||
|} | |} | ||
| + | |||
| + | [[Image:160.jpg|none|thumb|500px|The estimated probability distribution for LTC4S concentration. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale. ]] | ||
=== K<sub>eq</sub>=== | === K<sub>eq</sub>=== | ||
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|} | |} | ||
| + | [[Image:56.jpg|none|thumb|500px|The estimated probability distribution for LTC4S Keq. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale. ]] | ||
== Related Reactions == | == Related Reactions == | ||
Revision as of 14:04, 15 May 2019
To generate LTC4, a supramolecular complex of 5-LOX, FLAP and leukotriene C4 synthase is formed on the nuclear membrane due to the increase of intracellular calcium \cite{Woods1993, Hammarberg2000, Radmark2015, Evans2008, Mandal2004, Mandal2008}
Contents
Reaction
Chemical equation
Rate equation
Parameters
Kms
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 0.3 �± 0.06 | mM | Human | Expression Vector: E. Coli.
Enzyme: Wild Type hLTC4S pH:7.8 Temperature:20 °C |
[1] |
| 3.00E-02 ± 1.00E-02 | mM | Human | Expression Vector: E Coli
Enzyme: Wild type LTC4S pH: 7.8 Temperature: 37 °C |
[2] |
| Mode (mM) | Confidence Interval | Location parameter (µ) | Scale parameter (σ) |
|---|---|---|---|
| 3.15E-02 | 7.18E+00 | -2.83E+00 | 7.90E-01 |
Kmp
| Mode (mM) | Location parameter (µ) | Scale parameter (σ) |
|---|---|---|
| 3.18E-02 | -2.82E+00 | 7.92E-01 |
kcat
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 702 | per minute | Human | Expression Vector: E. Coli.
Enzyme: Wild Type hLTC4S pH:7.8 Temperature:20 °C |
[1] |
| 1560 ± 240 | per minute | Human | Expression Vector: E Coli
Enzyme: Wild type LTC4S pH: 7.8 Temperature: 37 °C |
[2] |
| Mode (min-1) | Confidence Interval | Location parameter (µ) | Scale parameter (σ) |
|---|---|---|---|
| 1.47E+03 | 1.42E+00 | 7.40E+00 | 3.29E-01 |
Enzyme concentration
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 26.8 | 
|
Human | Expression Vector: Lung
Enzyme: LTC4S pH: 7.5 Temperature: 37 °C |
[3] |
| 33.0 |
|
Human | Expression Vector: Esophagus
Enzyme: LTC4S pH: 7.5 Temperature: 37 °C |
[3] |
| 13.9 |
|
Human | Expression Vector: Adrenal Gland
Enzyme: LTC4S pH: 7.5 Temperature: 37 °C |
[3] |
| Mode | Confidence Interval | Location parameter (µ) | Scale parameter (σ) |
|---|---|---|---|
| 2.90E+01 | 1.44E+00 | 3.49E+00 | 3.46E-01 |
Keq
| Gibbs Free Energy Change | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 9.934128 | kcal/mol | Not stated | Estimated
Enzyme: LTC4S Substrate: LTA4 Product: LTC4 pH: 7.3 ionic strength: 0.25 |
[4] |
| Mode (mM) | Confidence Interval | Location parameter (µ) | Scale parameter (σ) |
|---|---|---|---|
| 5.13E-08 | 1.00E+01 | -1.60E+01 | 8.90E-01 |
Related Reactions
References
- ↑ 1.0 1.1 [http://www.jbc.org/content/285/52/40771.full.pdf Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4 Synthase J Biochem 2010, 285, 40771-40776]
- ↑ 2.0 2.1 [http://www.jbc.org/content/early/2013/12/23/jbc.M113.534628 Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the
enzyme mechanism J. Biol. Chem. 289, 5199-5207 (2014)] Cite error: Invalid
<ref>tag; name "Niegowski2013" defined multiple times with different content - ↑ 3.0 3.1 3.2 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
- ↑ Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471
