Difference between revisions of "Transformation of LTA4 to LTC4"

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(→‎Parameters)
(→‎Parameters)
Line 25: Line 25:
 
|mM
 
|mM
 
|Human
 
|Human
−
|Wild Type hLTC4S, 20 °C, pH 7.8, incubated for 15 seconds.
+
|Expression Vector: E. Coli.
 +
Enzyme: Wild Type hLTC4S
 +
pH:7.8
 +
Temperature:20 °C
 
|<ref name="Rinaldo2010"> [http://www.jbc.org/content/285/52/40771.full.pdf  Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4
 
|<ref name="Rinaldo2010"> [http://www.jbc.org/content/285/52/40771.full.pdf  Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4
 
Synthase'' J Biochem 2010, 285, 40771-40776]</ref>
 
Synthase'' J Biochem 2010, 285, 40771-40776]</ref>
Line 32: Line 35:
 
|mM
 
|mM
 
|Human
 
|Human
−
|Wild type, pH 7.8
+
|Expression Vector: E Coli
−
|<ref name="Niegowski2013"> [http://www.ncbi.nlm.nih.gov/pubmed/19010934 Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the
+
Enzyme: Wild type LTC4S
 +
pH: 7.8
 +
Temperature: 37 °C
 +
|<ref name="Niegowski2013"> [http://www.jbc.org/content/early/2013/12/23/jbc.M113.534628 Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the
 
enzyme mechanism'' J. Biol. Chem. 289, 5199-5207 (2014)]</ref>
 
enzyme mechanism'' J. Biol. Chem. 289, 5199-5207 (2014)]</ref>
 
|-
 
|-
Line 50: Line 56:
 
|per minute
 
|per minute
 
|Human
 
|Human
−
|Wild Type hLTC4S, 20 °C, pH 7.8, incubated for 15 seconds.
+
|Expression Vector: E. Coli.
 +
Enzyme: Wild Type hLTC4S
 +
pH:7.8
 +
Temperature:20 °C
 
|<ref name="Rinaldo2010"> [http://www.jbc.org/content/285/52/40771.full.pdf  Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4
 
|<ref name="Rinaldo2010"> [http://www.jbc.org/content/285/52/40771.full.pdf  Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4
 
Synthase'' J Biochem 2010, 285, 40771-40776]</ref>
 
Synthase'' J Biochem 2010, 285, 40771-40776]</ref>
Line 57: Line 66:
 
|per minute
 
|per minute
 
|Human
 
|Human
−
|Wild Type, pH 7.8
+
|Expression Vector: E Coli
 +
Enzyme: Wild type LTC4S
 +
pH: 7.8
 +
Temperature: 37 °C
 
|<ref name="Niegowski2013"> [http://www.jbc.org/content/289/8/5199.full Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the
 
|<ref name="Niegowski2013"> [http://www.jbc.org/content/289/8/5199.full Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the
 
enzyme mechanism'' J. Biol. Chem. 289, 5199-5207 (2014)]</ref>
 
enzyme mechanism'' J. Biol. Chem. 289, 5199-5207 (2014)]</ref>
Line 75: Line 87:
 
|mM
 
|mM
 
|Human
 
|Human
−
|Calculated from 20ug of 16.5kDa protein.
+
|Expression Vector: E. Coli.
−
Wild Type hLTC4S, 20 °C, pH 7.8, incubated for 15 seconds.
+
Enzyme: Wild Type hLTC4S
 +
pH:7.8
 +
Temperature:20 °C
 +
Calculated from 20ug of 16.5kDa protein.
 
|<ref name="Rinaldo2010"> [http://www.jbc.org/content/285/52/40771.full.pdf Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4
 
|<ref name="Rinaldo2010"> [http://www.jbc.org/content/285/52/40771.full.pdf Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4
 
Synthase'' J Biochem 2010, 285, 40771-40776]</ref>
 
Synthase'' J Biochem 2010, 285, 40771-40776]</ref>
Line 83: Line 98:
 
|mM
 
|mM
 
|Human
 
|Human
−
|Wild Type, pH 7.8
+
|Expression Vector: E Coli
 +
Enzyme: Wild type LTC4S
 +
pH: 7.8
 +
Temperature: 37 °C
 
|<ref name="Niegowski2013"> [http://www.jbc.org/content/289/8/5199.full Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the
 
|<ref name="Niegowski2013"> [http://www.jbc.org/content/289/8/5199.full Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the
 
enzyme mechanism'' J. Biol. Chem. 289, 5199-5207 (2014)]</ref>
 
enzyme mechanism'' J. Biol. Chem. 289, 5199-5207 (2014)]</ref>

Revision as of 13:41, 9 June 2016

Return to overview

Reaction

Chemical equation

AA \rightleftharpoons PGH2

Rate equation

Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
0.3 �± 0.06 mM Human Expression Vector: E. Coli.

Enzyme: Wild Type hLTC4S pH:7.8 Temperature:20 °C

[1]
3.00E-02 ± 1.00E-02 mM Human Expression Vector: E Coli

Enzyme: Wild type LTC4S pH: 7.8 Temperature: 37 °C

[2]
Enzyme Turnover Numbers
Value Units Species Notes Reference
702 per minute Human Expression Vector: E. Coli.

Enzyme: Wild Type hLTC4S pH:7.8 Temperature:20 °C

[1]
1560 ± 240 per minute Human Expression Vector: E Coli

Enzyme: Wild type LTC4S pH: 7.8 Temperature: 37 °C

[2]
Enzyme Turnover Numbers
Value Units Species Notes Reference
1.21E-04 mM Human Expression Vector: E. Coli.

Enzyme: Wild Type hLTC4S pH:7.8 Temperature:20 °C Calculated from 20ug of 16.5kDa protein.

[1]
6.06e-05 - 3.03e-05 mM Human Expression Vector: E Coli

Enzyme: Wild type LTC4S pH: 7.8 Temperature: 37 °C

[2]

References

  1. ↑ 1.0 1.1 1.2 [http://www.jbc.org/content/285/52/40771.full.pdf Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4 Synthase J Biochem 2010, 285, 40771-40776] Cite error: Invalid <ref> tag; name "Rinaldo2010" defined multiple times with different content
  2. ↑ 2.0 2.1 2.2 [http://www.jbc.org/content/early/2013/12/23/jbc.M113.534628 Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the enzyme mechanism J. Biol. Chem. 289, 5199-5207 (2014)] Cite error: Invalid <ref> tag; name "Niegowski2013" defined multiple times with different content Cite error: Invalid <ref> tag; name "Niegowski2013" defined multiple times with different content

Related Reactions

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