Difference between revisions of "Transformation of LTA4 to LTC4"
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|Human | |Human | ||
|Wild Type hLTC4S, 20 °C, pH 7.8, incubated for 15 seconds. | |Wild Type hLTC4S, 20 °C, pH 7.8, incubated for 15 seconds. | ||
| − | |<ref name="Rinaldo2010"> [http://www. | + | |<ref name="Rinaldo2010"> [http://www.jbc.org/content/285/52/40771.full.pdf Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4 |
Synthase'' J Biochem 2010, 285, 40771-40776]</ref> | Synthase'' J Biochem 2010, 285, 40771-40776]</ref> | ||
|- | |- | ||
| Line 51: | Line 51: | ||
|Human | |Human | ||
|Wild Type hLTC4S, 20 °C, pH 7.8, incubated for 15 seconds. | |Wild Type hLTC4S, 20 °C, pH 7.8, incubated for 15 seconds. | ||
| − | |<ref name="Rinaldo2010"> [http://www. | + | |<ref name="Rinaldo2010"> [http://www.jbc.org/content/285/52/40771.full.pdf Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4 |
Synthase'' J Biochem 2010, 285, 40771-40776]</ref> | Synthase'' J Biochem 2010, 285, 40771-40776]</ref> | ||
|- | |- | ||
| Line 58: | Line 58: | ||
|Human | |Human | ||
|Wild Type, pH 7.8 | |Wild Type, pH 7.8 | ||
| − | |<ref name="Niegowski2013"> [http://www. | + | |<ref name="Niegowski2013"> [http://www.jbc.org/content/289/8/5199.full Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the |
enzyme mechanism'' J. Biol. Chem. 289, 5199-5207 (2014)]</ref> | enzyme mechanism'' J. Biol. Chem. 289, 5199-5207 (2014)]</ref> | ||
|- | |- | ||
|} | |} | ||
| + | {|class="wikitable sortable" | ||
| + | |+ style="text-align: left;" | Enzyme Turnover Numbers | ||
| + | |- | ||
| + | ! Value | ||
| + | ! Units | ||
| + | ! Species | ||
| + | ! Notes | ||
| + | ! Reference | ||
| + | |- | ||
| + | |1.21E-04 | ||
| + | |mM | ||
| + | |Human | ||
| + | |Calculated from 20ug of 16.5kDa protein. | ||
| + | Wild Type hLTC4S, 20 °C, pH 7.8, incubated for 15 seconds. | ||
| + | |<ref name="Rinaldo2010"> [http://www.jbc.org/content/285/52/40771.full.pdf Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4 | ||
| + | Synthase'' J Biochem 2010, 285, 40771-40776]</ref> | ||
| + | |- | ||
| + | |6.06e-05 - 3.03e-05 | ||
| + | |Human | ||
| + | |Wild Type, pH 7.8 | ||
| + | |<ref name="Niegowski2013"> [http://www.jbc.org/content/289/8/5199.full Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the | ||
| + | enzyme mechanism'' J. Biol. Chem. 289, 5199-5207 (2014)]</ref> | ||
| + | |- | ||
| + | |} | ||
==References== | ==References== | ||
Revision as of 11:50, 27 April 2016
Contents
Reaction
Chemical equation
Rate equation
Parameters
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 0.3 �± 0.06 | mM | Human | Wild Type hLTC4S, 20 °C, pH 7.8, incubated for 15 seconds. | [1] |
| 3.00E-02 ± 1.00E-02 | mM | Human | Wild type, pH 7.8 | [2] |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 702 | per minute | Human | Wild Type hLTC4S, 20 °C, pH 7.8, incubated for 15 seconds. | [1] |
| 1560 ± 240 | per minute | Human | Wild Type, pH 7.8 | [2] |
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 1.21E-04 | mM | Human | Calculated from 20ug of 16.5kDa protein.
Wild Type hLTC4S, 20 °C, pH 7.8, incubated for 15 seconds. |
[1] |
| 6.06e-05 - 3.03e-05 | Human | Wild Type, pH 7.8 | [2] |
References
- ↑ 1.0 1.1 1.2 [http://www.jbc.org/content/285/52/40771.full.pdf Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4
Synthase J Biochem 2010, 285, 40771-40776] Cite error: Invalid
<ref>tag; name "Rinaldo2010" defined multiple times with different content - ↑ 2.0 2.1 2.2 [http://www.ncbi.nlm.nih.gov/pubmed/19010934 Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the
enzyme mechanism J. Biol. Chem. 289, 5199-5207 (2014)] Cite error: Invalid
<ref>tag; name "Niegowski2013" defined multiple times with different content Cite error: Invalid<ref>tag; name "Niegowski2013" defined multiple times with different content
