Difference between revisions of "Transformation of 5-HETE to 5-OXO-ETE"
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− | |+ style="text-align: left;" | 5-HEDH Abundance | + | |+ style="text-align: left;" | 5-HEDH Abundance (Using ADH5 abundance data) |
! Value | ! Value | ||
! Units | ! Units | ||
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! Reference | ! Reference | ||
|- | |- | ||
− | | | + | | 490 |
− | | | + | | ppm |
− | | | + | | Human |
− | | A | + | |Expression Vector: Skin |
− | | | + | Enzyme: ADH5 |
+ | pH: 7.5 | ||
+ | Temperature: 37 °C | ||
+ | |<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the | ||
+ | human proteome'' Nature, 2014 509, 582–587]</ref> | ||
+ | |- | ||
+ | |625 | ||
+ | |ppm | ||
+ | |Human | ||
+ | |Expression Vector: Oral Cavity | ||
+ | Enzyme: ADH5 | ||
+ | pH: 7.5 | ||
+ | Temperature: 37 °C | ||
+ | |<ref name="Wilhelm2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm ''Mass-spectrometry-based draft of the | ||
+ | human proteome'' Nature, 2014 509, 582–587]</ref> | ||
+ | |- | ||
+ | |728 | ||
+ | |ppm | ||
+ | |Human | ||
+ | |Expression Vector: Esophagus | ||
+ | Enzyme: ADH5 | ||
+ | pH: 7.5 | ||
+ | Temperature: 37 °C | ||
+ | |<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref> | ||
+ | |- | ||
+ | |11.5 | ||
+ | |ppm | ||
+ | |Human | ||
+ | |Expression Vector: Skin | ||
+ | Enzyme: ADH5 | ||
+ | pH: Unknown | ||
+ | Temperature: Unknown | ||
+ | |Unknown Paper, from Paxdb database. | ||
+ | |- | ||
|} | |} | ||
Revision as of 15:19, 15 March 2017
The metabolism of 5-HETE to 5-Oxo-ETE is catalysed by the enzyme 5-Hydroxyeicosanoid dehydrogenase (5-HEDH). This enzyme acts reversibly and has been reported to be found in eosinophils, monocytes, DC, B-lymphocytes, keratinocytes and platelets (Steinhilber book) *skin (need to check). The product binds with the OXE receptor (Hosoi2002) and is 30-100 more bioactive than 5-HETE. 5-Oxo-ETE is a chemoattractant for neutrophils and eosinophils.
Contents
Reaction
Chemical equation
Rate equation
Parameters
Note: Limited availability of kinetic data for specific enzyme, using general alcohol dehydrogenase (ADH) data when non is available.
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
0.0006 | mM | Unkown | Follows a ping-pong mechanism by binding with NADP+, transforming it to NADPH and releasing it before binding 5S-HETE. | [1] |
0.00067 | mM | Human Cell lines | Follows a ping-pong mechanism by binding with NADP+ (Km 139 nM), transforming it to NADPH (inhibited by NADPH (Ki 224 nM)) and releasing it before binding 5S-HETE.
Method: In vitro Organism: Human Expression vector: U937 and HL-60 cell lines Enzyme:5-HEDH pH: 7.4 Temperature: 37 ◦C |
[2] |
0.000516 ± 0.00019 | mM | Human Cell lines |
Method: In vitro Organism: Human Expression vector: U937 Enzyme:5-HEDH pH: 7.4 Temperature: 37 ◦C |
[3] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
0.54 ± 0.30 | pmol/ mi mg | Human Cell lines - Neutrophils |
Method: In vitro Organism: Human Expression vector: U937 and HL-60 cell lines Enzyme:5-HEDH pH: 7.4 Temperature: 37 ◦C |
[2] |
0.40 ± 0.12 | pmol/ mi mg | Human Cell lines - U937 |
Method: In vitro Organism: Human Expression vector: U937 and HL-60 cell lines Enzyme:5-HEDH pH: 7.4 Temperature: 37 ◦C |
[2] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
11640 ± 840 | min-1 | Yokenella | Method: In vitro
Organism: Human Expression vector: E Coli Enzyme: pH: 6.5 Temperature: 65 ◦C Substrate: NADPH |
B |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
490 | ppm | Human | Expression Vector: Skin
Enzyme: ADH5 pH: 7.5 Temperature: 37 °C |
[4] |
625 | ppm | Human | Expression Vector: Oral Cavity
Enzyme: ADH5 pH: 7.5 Temperature: 37 °C |
[4] |
728 | ppm | Human | Expression Vector: Esophagus
Enzyme: ADH5 pH: 7.5 Temperature: 37 °C |
[5] |
11.5 | ppm | Human | Expression Vector: Skin
Enzyme: ADH5 pH: Unknown Temperature: Unknown |
Unknown Paper, from Paxdb database. |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
1.29 | kcal/mol | Arabidopsis thaliana col | Same reaction, no prediction available for 5-HEDH on Metacyc
Estimated Enzyme: ω-hydroxy fatty acid ω-alcohol dehydrogenase Substrate: 18-hydroxyoleate Product: 18-oxo-oleate pH: 7.3 ionic strength: 0.25 |
[6] |
Related Reactions
References
- ↑ Lipoxygenases in Inflammation - edited by Dieter Steinhilber
- ↑ 2.0 2.1 2.2 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828885/pdf/bj4030157.pdf Karl-Rudolf ERLEMANN, Regulation of 5-hydroxyeicosanoid dehydrogenase activity in monocytic cells, Biochem. J. (2007) 403, 157–165]
- ↑ [http://jpet.aspetjournals.org/content/jpet/329/1/335.full.pdf Karl-Pranav Patel, Selectivity of 5-Hydroxyeicosanoid Dehydrogenase and Its Inhibition by 5-Hydroxy-Long-Chain Fatty Acids, Journal of Pharmacology and Experimental Therapeutics April 2009, 329 (1) 335-341; ]
- ↑ 4.0 4.1 [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13319.pdf M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587]
- ↑ M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
- ↑ Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471