Difference between revisions of "Transformation of 5-HETE to 5-OXO-ETE"

From ISMOC
Jump to: navigation, search
(→‎Parameters)
Line 60: Line 60:
  
 
{|class="wikitable"  
 
{|class="wikitable"  
−
|+  style="text-align: left;" | Michaelis-Menten Constants
+
|+  style="text-align: left;" | Vmax
 
! Value
 
! Value
 
! Units
 
! Units
Line 67: Line 67:
 
! Reference
 
! Reference
 
|-
 
|-
−
| X
+
| 0.54 ± 0.30
−
| Y
+
| pmol/ mi mg
−
| Z
+
| Human Cell lines - Neutrophils
−
| A
+
|
−
| B
+
Method: In vitro
 +
Organism: Human
 +
Expression vector: U937 and HL-60 cell lines
 +
Enzyme:5-HEDH
 +
pH: 7.4
 +
Temperature:  37 ◦C
 +
| <ref name="ERLEMANN2007"> [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828885/pdf/bj4030157.pdf Karl-Rudolf ERLEMANN, Regulation of 5-hydroxyeicosanoid dehydrogenase activity in
 +
monocytic cells, Biochem. J. (2007) 403, 157–165]</ref>
 +
|-
 +
| 0.40 ± 0.12
 +
| pmol/ mi mg
 +
| Human Cell lines - U937
 +
|
 +
Method: In vitro
 +
Organism: Human
 +
Expression vector: U937 and HL-60 cell lines
 +
Enzyme:5-HEDH
 +
pH: 7.4
 +
Temperature:  37 ◦C
 +
| <ref name="ERLEMANN2007"> [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828885/pdf/bj4030157.pdf Karl-Rudolf ERLEMANN, Regulation of 5-hydroxyeicosanoid dehydrogenase activity in
 +
monocytic cells, Biochem. J. (2007) 403, 157–165]</ref>
 +
|-
 
|}
 
|}
  

Revision as of 10:55, 13 March 2017

Return to overview

The metabolism of 5-HETE to 5-Oxo-ETE is catalysed by the enzyme 5-Hydroxyeicosanoid dehydrogenase (5-HEDH). This enzyme acts reversibly and has been reported to be found in eosinophils, monocytes, DC, B-lymphocytes, keratinocytes and platelets (Steinhilber book) *skin (need to check). The product binds with the OXE receptor (Hosoi2002) and is 30-100 more bioactive than 5-HETE. 5-Oxo-ETE is a chemoattractant for neutrophils and eosinophils.

Doesn't com up in BRENDA or PAXDB - Skeptical

Reaction

R14 HETE5-OETE5.jpg

Chemical equation

5-HETE \rightleftharpoons 5-Oxo-ETE

Rate equation

Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
0.0006 mM Unkown Follows a ping-pong mechanism by binding with NADP+, transforming it to NADPH and releasing it before binding 5S-HETE. [1]
0.00067 mM Human Cell lines Follows a ping-pong mechanism by binding with NADP+ (Km 139 nM), transforming it to NADPH (inhibited by NADPH (Ki 224 nM)) and releasing it before binding 5S-HETE.

Method: In vitro Organism: Human Expression vector: U937 and HL-60 cell lines Enzyme:5-HEDH pH: 7.4 Temperature: 37 ◦C

[2]
0.000516 ± 0.00019 mM Human Cell lines

Method: In vitro Organism: Human Expression vector: U937 Enzyme:5-HEDH pH: 7.4 Temperature: 37 ◦C

[3]
Vmax
Value Units Species Notes Reference
0.54 ± 0.30 pmol/ mi mg Human Cell lines - Neutrophils

Method: In vitro Organism: Human Expression vector: U937 and HL-60 cell lines Enzyme:5-HEDH pH: 7.4 Temperature: 37 ◦C

[2]
0.40 ± 0.12 pmol/ mi mg Human Cell lines - U937

Method: In vitro Organism: Human Expression vector: U937 and HL-60 cell lines Enzyme:5-HEDH pH: 7.4 Temperature: 37 ◦C

[2]
Enzyme Turnover Number
Value Units Species Notes Reference
X Y Z A B
5-HEDH Abundance
Value Units Species Notes Reference
X Y Z A B
Gibbs Free energy
Value Units Species Notes Reference
X Y Z A B

Related Reactions

References

  1. ↑ Lipoxygenases in Inflammation - edited by Dieter Steinhilber
  2. ↑ 2.0 2.1 2.2 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828885/pdf/bj4030157.pdf Karl-Rudolf ERLEMANN, Regulation of 5-hydroxyeicosanoid dehydrogenase activity in monocytic cells, Biochem. J. (2007) 403, 157–165]
  3. ↑ [http://jpet.aspetjournals.org/content/jpet/329/1/335.full.pdf Karl-Pranav Patel, Selectivity of 5-Hydroxyeicosanoid Dehydrogenase and Its Inhibition by 5-Hydroxy-Long-Chain Fatty Acids, Journal of Pharmacology and Experimental Therapeutics April 2009, 329 (1) 335-341; ]
Retrieved from ‘http://www.systemsbiology.ls.manchester.ac.uk/wiki/index.php?title=Transformation_of_5-HETE_to_5-OXO-ETE&oldid=5156’