Difference between revisions of "Synthesis of C"
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|<math>K_{C}</math> | |<math>K_{C}</math> | ||
|<math>0.1 - 0.2 </math> <ref name="Schaefer1996"> Schaefer AL, Val DL, Hanzelka BL, Cronan JE, Greenberg EP. ''Generation of cell-to-cell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein.'' PNAS 1996;93(18):9505-9509.</ref> <ref name="Mathew1999"> Matthew R. Parsek, Dale L. Val, Brian L. Hanzelka, John E. Cronan, Jr., and E. P. Greenberg. ''Acyl homoserine-lactone quorum-sensing signal generation''.PNAS 1999;96(8):4360-4365</ref> <ref name="Garcia2004"> J.Garcia-Ojalvo, M. B. Elowitz, and S. H. Strogatz. ''Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing''.PNAS 2004;101(30):10955-10960; published ahead of print July 15, 2004.</ref> <ref name="Chen2004"> Chun-Chiang Chen, Lieke Riadi, Sang-Jin Suh, Dennis E. Ohman, and Lu-Kwang Ju. "Degradation and synthesis kinetics of quorum-sensing autoinducer in ''Pseudomonas aeruginosa'' cultivation. J. Biotechnol. 2005; 117:1-10. </ref> | |<math>0.1 - 0.2 </math> <ref name="Schaefer1996"> Schaefer AL, Val DL, Hanzelka BL, Cronan JE, Greenberg EP. ''Generation of cell-to-cell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein.'' PNAS 1996;93(18):9505-9509.</ref> <ref name="Mathew1999"> Matthew R. Parsek, Dale L. Val, Brian L. Hanzelka, John E. Cronan, Jr., and E. P. Greenberg. ''Acyl homoserine-lactone quorum-sensing signal generation''.PNAS 1999;96(8):4360-4365</ref> <ref name="Garcia2004"> J.Garcia-Ojalvo, M. B. Elowitz, and S. H. Strogatz. ''Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing''.PNAS 2004;101(30):10955-10960; published ahead of print July 15, 2004.</ref> <ref name="Chen2004"> Chun-Chiang Chen, Lieke Riadi, Sang-Jin Suh, Dennis E. Ohman, and Lu-Kwang Ju. "Degradation and synthesis kinetics of quorum-sensing autoinducer in ''Pseudomonas aeruginosa'' cultivation. J. Biotechnol. 2005; 117:1-10. </ref> | ||
| − | |<math> s-{-1} </math> | + | |<math> s-^{-}_{1} </math> |
| Value reported for Acyl homoserine lactone (AHL) in ''Vibrio fischeri'' and ''E. coli''. Reported synthesis rate for AHL PAI2 from ''P. aeruginosa'' at stationary phase. This was calculated by subtracting the <math>k_{deg}</math> from the net decay constant (<math>k_{deg}-k_{syn}</math>). | | Value reported for Acyl homoserine lactone (AHL) in ''Vibrio fischeri'' and ''E. coli''. Reported synthesis rate for AHL PAI2 from ''P. aeruginosa'' at stationary phase. This was calculated by subtracting the <math>k_{deg}</math> from the net decay constant (<math>k_{deg}-k_{syn}</math>). | ||
| The value was chosen with the assumption that GBL production involves one rate | | The value was chosen with the assumption that GBL production involves one rate | ||
Revision as of 16:17, 2 October 2015
SCB (C) is synthesized by glycerol derivative and b-keto acid derivative precursors in a reaction catalyzed by ScbA (A).
Contents
Chemical equation
Rate equation
In our model we assume that there is an infinite amount of substrate S for the SCB synthesis and that the rate of the reaction is dependent only on the concentration of the catalyst A. Therefore, the rate of the reaction is:
![r= K_{C}\cdot [A]](/wiki/images/math/d/7/9/d796407593b5d6ed432d122e52d6a2a1.png)
Parameters
The parameter of this reaction is the synthesis rate of SCB (
).
| Name | Value | Units | Origin | Remarks |
|---|---|---|---|---|
|
|
Failed to parse (Cannot store math image on filesystem.): 0.1 - 0.2 [1] [2] [3] [4] | Failed to parse (Cannot store math image on filesystem.): s-^{-}_{1} | Value reported for Acyl homoserine lactone (AHL) in Vibrio fischeri and E. coli. Reported synthesis rate for AHL PAI2 from P. aeruginosa at stationary phase. This was calculated by subtracting the  from the net decay constant ( ).
|
The value was chosen with the assumption that GBL production involves one rate
limiting step involving the Afsa-like protein and then spontaneous cyclization. |
Parameters with uncertainty
The most plausible parameter value for the is decided to be 
and the confidence interval 
. This means that the mode of the PDF is ?? and the range where 95% of the values are found is between ?? and ?? 
.
The probability distributions for the parameter, adjusted accordingly in order to reflect the above values, is the following:
The location and scale parameters of the distribution are:
| Parameter | μ | σ |
|---|---|---|
|
|
|
|
References
- ↑ Schaefer AL, Val DL, Hanzelka BL, Cronan JE, Greenberg EP. Generation of cell-to-cell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein. PNAS 1996;93(18):9505-9509.
- ↑ Matthew R. Parsek, Dale L. Val, Brian L. Hanzelka, John E. Cronan, Jr., and E. P. Greenberg. Acyl homoserine-lactone quorum-sensing signal generation.PNAS 1999;96(8):4360-4365
- ↑ J.Garcia-Ojalvo, M. B. Elowitz, and S. H. Strogatz. Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing.PNAS 2004;101(30):10955-10960; published ahead of print July 15, 2004.
- ↑ Chun-Chiang Chen, Lieke Riadi, Sang-Jin Suh, Dennis E. Ohman, and Lu-Kwang Ju. "Degradation and synthesis kinetics of quorum-sensing autoinducer in Pseudomonas aeruginosa cultivation. J. Biotechnol. 2005; 117:1-10.
