Difference between revisions of "Synthesis of C"
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|<math>0.1 - 0.2 </math> <ref name="Schaefer1996"> Schaefer AL, Val DL, Hanzelka BL, Cronan JE, Greenberg EP. ''Generation of cell-to-cell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein.'' PNAS 1996;93(18):9505-9509.</ref> <ref name="Mathew1999"> Matthew R. Parsek, Dale L. Val, Brian L. Hanzelka, John E. Cronan, Jr., and E. P. Greenberg. ''Acyl homoserine-lactone quorum-sensing signal generation''.PNAS 1999;96(8):4360-4365</ref> <ref name="Garcia2004"> J.Garcia-Ojalvo, M. B. Elowitz, and S. H. Strogatz. ''Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing''.PNAS 2004;101(30):10955-10960; published ahead of print July 15, 2004.</ref> <ref name="Chen2004"> Chun-Chiang Chen, Lieke Riadi, Sang-Jin Suh, Dennis E. Ohman, and Lu-Kwang Ju. "Degradation and synthesis kinetics of quorum-sensing autoinducer in ''Pseudomonas aeruginosa'' cultivation. J. Biotechnol. 2005; 117:1-10. </ref> | |<math>0.1 - 0.2 </math> <ref name="Schaefer1996"> Schaefer AL, Val DL, Hanzelka BL, Cronan JE, Greenberg EP. ''Generation of cell-to-cell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein.'' PNAS 1996;93(18):9505-9509.</ref> <ref name="Mathew1999"> Matthew R. Parsek, Dale L. Val, Brian L. Hanzelka, John E. Cronan, Jr., and E. P. Greenberg. ''Acyl homoserine-lactone quorum-sensing signal generation''.PNAS 1999;96(8):4360-4365</ref> <ref name="Garcia2004"> J.Garcia-Ojalvo, M. B. Elowitz, and S. H. Strogatz. ''Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing''.PNAS 2004;101(30):10955-10960; published ahead of print July 15, 2004.</ref> <ref name="Chen2004"> Chun-Chiang Chen, Lieke Riadi, Sang-Jin Suh, Dennis E. Ohman, and Lu-Kwang Ju. "Degradation and synthesis kinetics of quorum-sensing autoinducer in ''Pseudomonas aeruginosa'' cultivation. J. Biotechnol. 2005; 117:1-10. </ref> | ||
|<math> s-1 </math> | |<math> s-1 </math> | ||
| − | | Value reported for Acyl homoserine lactone (AHL) | + | | Value reported for Acyl homoserine lactone (AHL). Reported synthesis rate for AHL PAI2 from ''P. aeruginosa'' at stationary phase. This was calculated by subtracting the k_{deg} from the net decay constant (k_{deg}-k_{syn}). |
| − | |||
in ''Vibrio fischeri'' and ''E. coli'' | in ''Vibrio fischeri'' and ''E. coli'' | ||
| The value was chosen with the assumption that GBL production involves one rate | | The value was chosen with the assumption that GBL production involves one rate | ||
Revision as of 16:13, 2 October 2015
SCB (C) is synthesized by glycerol derivative and b-keto acid derivative precursors in a reaction catalyzed by ScbA (A).
Contents
Chemical equation
Rate equation
In our model we assume that there is an infinite amount of substrate S for the SCB synthesis and that the rate of the reaction is dependent only on the concentration of the catalyst A. Therefore, the rate of the reaction is:
![r= K_{C}\cdot [A]](/wiki/images/math/d/7/9/d796407593b5d6ed432d122e52d6a2a1.png)
Parameters
The parameter of this reaction is the synthesis rate of SCB (
).
| Name | Value | Units | Origin | Remarks |
|---|---|---|---|---|
|
|
Failed to parse (Cannot store math image on filesystem.): 0.1 - 0.2 [1] [2] [3] [4] | Failed to parse (Cannot store math image on filesystem.): s-1 | Value reported for Acyl homoserine lactone (AHL). Reported synthesis rate for AHL PAI2 from P. aeruginosa at stationary phase. This was calculated by subtracting the k_{deg} from the net decay constant (k_{deg}-k_{syn}).
in Vibrio fischeri and E. coli |
The value was chosen with the assumption that GBL production involves one rate
limiting step involving the Afsa-like protein and then spontaneous cyclization. |
Parameters with uncertainty
The most plausible parameter value for the is decided to be 
and the confidence interval 
. This means that the mode of the PDF is ?? and the range where 95% of the values are found is between ?? and ?? 
.
The probability distributions for the parameter, adjusted accordingly in order to reflect the above values, is the following:
The location and scale parameters of the distribution are:
| Parameter | μ | σ |
|---|---|---|
|
|
|
|
References
- ↑ Schaefer AL, Val DL, Hanzelka BL, Cronan JE, Greenberg EP. Generation of cell-to-cell signals in quorum sensing: acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein. PNAS 1996;93(18):9505-9509.
- ↑ Matthew R. Parsek, Dale L. Val, Brian L. Hanzelka, John E. Cronan, Jr., and E. P. Greenberg. Acyl homoserine-lactone quorum-sensing signal generation.PNAS 1999;96(8):4360-4365
- ↑ J.Garcia-Ojalvo, M. B. Elowitz, and S. H. Strogatz. Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing.PNAS 2004;101(30):10955-10960; published ahead of print July 15, 2004.
- ↑ Chun-Chiang Chen, Lieke Riadi, Sang-Jin Suh, Dennis E. Ohman, and Lu-Kwang Ju. "Degradation and synthesis kinetics of quorum-sensing autoinducer in Pseudomonas aeruginosa cultivation. J. Biotechnol. 2005; 117:1-10.
