COX-2 Induction Event

The induced expression of COX-2. As this process is as a result of complex transcription and translation processes, this reaction was simplified.

Reaction

Parameter Name	Parameter Shorthand	Initial Value	Justification
Doubling time of the COX-2 event	COX2DT	180 min	This value was upon the unpublished western blot data in (Kiezel-Tsugunova, 2017).
Maximum concentration of COX-2	C3	0 mM	The adapted value of this parameter depends on the scenario (Table *). This value changes 240 min into the simulation for the UVR scenarios.
Decay switch	C4	0	If required the induced expression of COX-2 can decrease over time by amending this value from 0 to 1. This was set to 0 in all scenarios.
Half-life of the COX-2 event	COX2HL	0 min	This value was arbitrarily set at 0 min. However, no simulations required COX-2 release to decrease over time.

In silico experiment AA values
In silico experiment	HaCaT keratinocyte + A23187	46BR.1N fibroblast +A23187	HaCaT keratinocyte + Indomethacin and A23187	46BR.1N fibroblast + Indomethacin and A23187	HaCaT keratinocyte + ATP	46BR.1N fibroblast + ATP	HaCaT keratinocyte + UVR	46BR.1N fibroblast + UVR
COX-1 (mM) at 0h	x	x	1.00 x10^-28	1.00 x10^-28	x	x	x	x
COX-2 (mM) at 0h	x	x	1.00 x10^-28	1.00 x10^-28	x	x	1.00 x10^-28	1.00 x10^-28
COX-2 (mM) at 6h	x	x	x	x	x	x	2.27 x10^-2	2.27 x10^-2