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The induced expression of COX-2. As this process is as a result of complex transcription and translation processes, this reaction was simplified.
Reaction
Rate equation
Parameters
Parameter Name |
Parameter Shorthand |
Initial Value |
Justification
|
Doubling time of the COX-2 event |
COX2DT |
180 min |
This value was upon the unpublished western blot data in (Kiezel-Tsugunova, 2017).
|
Maximum concentration of COX-2 |
C3 |
0 mM |
The adapted value of this parameter depends on the scenario (Table *). This value changes 240 min into the simulation for the UVR scenarios.
|
Decay switch |
C4 |
0 |
If required the induced expression of COX-2 can decrease over time by amending this value from 0 to 1. This was set to 0 in all scenarios.
|
Half-life of the COX-2 event |
COX2HL |
0 min |
This value was arbitrarily set at 0 min. However, no simulations required COX-2 release to decrease over time.
|
In silico experiment AA values
In silico experiment |
HaCaT keratinocyte + A23187 |
46BR.1N fibroblast +A23187 |
HaCaT keratinocyte + Indomethacin and A23187 |
46BR.1N fibroblast + Indomethacin and A23187 |
HaCaT keratinocyte + ATP |
46BR.1N fibroblast + ATP |
HaCaT keratinocyte + UVR |
46BR.1N fibroblast + UVR
|
COX-1 (mM) at 0h |
x |
x |
1.00 x10-28 |
1.00 x10-28 |
x |
x |
x |
x
|
COX-2 (mM) at 0h |
x |
x |
1.00 x10-28 |
1.00 x10-28 |
x |
x |
1.00 x10-28 |
1.00 x10-28
|
COX-2 (mM) at 6h |
x |
x |
x |
x |
x |
x |
2.27 x10-2 |
2.27 x10-2
|