COX-2 Induction Event
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The induced expression of COX-2. As this process is as a result of complex transcription and translation processes, this reaction was simplified.
Reaction
Rate equation
Parameters
Parameter Name | Parameter Shorthand | Initial Value | Justification |
---|---|---|---|
Doubling time of the COX-2 event | COX2DT | 180 min | This value was upon the unpublished western blot data in (Kiezel-Tsugunova, 2017). |
Maximum concentration of COX-2 | C3 | 0 mM | The adapted value of this parameter depends on the scenario (Table *). This value changes 240 min into the simulation for the UVR scenarios. |
Decay switch | C4 | 0 | If required the induced expression of COX-2 can decrease over time by amending this value from 0 to 1. This was set to 0 in all scenarios. |
Half-life of the COX-2 event | COX2HL | 0 min | This value was arbitrarily set at 0 min. However, no simulations required COX-2 release to decrease over time. |
In silico experiment | HaCaT keratinocyte + A23187 | 46BR.1N fibroblast +A23187 | HaCaT keratinocyte + Indomethacin and A23187 | 46BR.1N fibroblast + Indomethacin and A23187 | HaCaT keratinocyte + ATP | 46BR.1N fibroblast + ATP | HaCaT keratinocyte + UVR | 46BR.1N fibroblast + UVR |
---|---|---|---|---|---|---|---|---|
COX-1 (mM) at 0h | x | x | 1.00 x10-28 | 1.00 x10-28 | x | x | x | x |
COX-2 (mM) at 0h | x | x | 1.00 x10-28 | 1.00 x10-28 | x | x | 1.00 x10-28 | 1.00 x10-28 |
COX-2 (mM) at 6h | x | x | x | x | x | x | 2.27 x10-2 | 2.27 x10-2 |