Difference between revisions of "ATP-Binding Cassette Transporters"
(→Parameters) |
(→Parameters) |
||
| Line 26: | Line 26: | ||
pH 7.0, 37°C, recombinant MRP2, using 4mM MgATP and 5g of isolated membranes. | pH 7.0, 37°C, recombinant MRP2, using 4mM MgATP and 5g of isolated membranes. | ||
|<ref name="Yas2007"> [http://ac.els-cdn.com/S0928098708003618/1-s2.0-S0928098708003618-main.pdf?_tid=a147bd90-1dc8-11e6-9405-00000aab0f26&acdnat=1463666001_5a08a971497da36ce8a604f84caadfc2 Yasunaga M. "Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2)'' Eur. J. Pharm. Sci. 35, 326-334 (2008)]</ref> | |<ref name="Yas2007"> [http://ac.els-cdn.com/S0928098708003618/1-s2.0-S0928098708003618-main.pdf?_tid=a147bd90-1dc8-11e6-9405-00000aab0f26&acdnat=1463666001_5a08a971497da36ce8a604f84caadfc2 Yasunaga M. "Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2)'' Eur. J. Pharm. Sci. 35, 326-334 (2008)]</ref> | ||
| + | |- | ||
| + | |0.0000366 ± 0.0000038 | ||
| + | |mM | ||
| + | |Human | ||
| + | |Substrate LTC4, 37 °C, 4 mM ATP, | ||
| + | |<ref name="Mao2000"> [http://www.jbc.org/content/275/44/34166.full.pdf Zeng H. "Transport of amphipathic anions by human multidrug resistance protein 3'' J. Biol. Chem. 275, 34166-34172 (2000)]</ref> | ||
| + | |- | ||
| + | |5.3E-3 ± 2.6E-3 | ||
| + | |mM | ||
| + | |Human | ||
| + | |4 mM ATP, 37°C, 5–10 ml of membrane vesicle suspension (30 | ||
| + | mg protein). | ||
| + | |<ref name="Zeng2000"> [http://cancerres.aacrjournals.org/content/60/17/4779.full.pdf "Functional reconstitution of substrate transport by purified multidrug resistance protein MRP1 (ABCC1) in phospholipid vesicles'' Cancer Res. 60, 4779-4784 (2000)]</ref> | ||
|- | |- | ||
|} | |} | ||
| Line 56: | Line 69: | ||
pH 7.0, 37°C, recombinant MRP2, using 4mM MgATP and 5g of isolated membranes. | pH 7.0, 37°C, recombinant MRP2, using 4mM MgATP and 5g of isolated membranes. | ||
|<ref name="Yas2007"> [http://ac.els-cdn.com/S0928098708003618/1-s2.0-S0928098708003618-main.pdf?_tid=a147bd90-1dc8-11e6-9405-00000aab0f26&acdnat=1463666001_5a08a971497da36ce8a604f84caadfc2 Yasunaga M. "Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2)'' Eur. J. Pharm. Sci. 35, 326-334 (2008)]</ref> | |<ref name="Yas2007"> [http://ac.els-cdn.com/S0928098708003618/1-s2.0-S0928098708003618-main.pdf?_tid=a147bd90-1dc8-11e6-9405-00000aab0f26&acdnat=1463666001_5a08a971497da36ce8a604f84caadfc2 Yasunaga M. "Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2)'' Eur. J. Pharm. Sci. 35, 326-334 (2008)]</ref> | ||
| + | |- | ||
| + | |125 ± 12 | ||
| + | |pmol/min/mg | ||
| + | |Human | ||
| + | |Substrate LTC4, 37 °C, 4 mM ATP, | ||
| + | |<ref name="Mao2000"> [http://www.jbc.org/content/275/44/34166.full.pdf Mao Q. "Functional reconstitution of substrate transport by purified multidrug resistance protein MRP1 (ABCC1) in phospholipid vesicles'' J. Biol. Chem. 275, 34166-34172 (2000)]</ref> | ||
| + | |- | ||
| + | |20.2 ± 5.9 | ||
| + | |pmol/mg/min | ||
| + | |Human | ||
| + | |4 mM ATP, 37°C, 5–10 ml of membrane vesicle suspension (30 | ||
| + | mg protein). | ||
| + | |<ref name="Zeng2000"> [http://cancerres.aacrjournals.org/content/60/17/4779.full.pdf "Functional reconstitution of substrate transport by purified multidrug resistance protein MRP1 (ABCC1) in phospholipid vesicles'' Cancer Res. 60, 4779-4784 (2000)]</ref> | ||
|- | |- | ||
|} | |} | ||
Revision as of 15:17, 19 May 2016
Contents
Reaction
Chemical equation
Rate equation
Parameters
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 0.0109 ± 0.00391 | mM | Human | Substrate: LTC4
pH 7.0, 37°C, recombinant MRP2, using 4mM MgATP and 5g of isolated membranes. |
[1] |
| 0.0000366 ± 0.0000038 | mM | Human | Substrate LTC4, 37 °C, 4 mM ATP, | [2] |
| 5.3E-3 ± 2.6E-3 | mM | Human | 4 mM ATP, 37°C, 5–10 ml of membrane vesicle suspension (30
mg protein). |
[3] |
| Value | Units | Species | Notes | Reference |
|---|
| Value | Units | Species | Notes | Reference |
|---|---|---|---|---|
| 190.9 ± 19.6 | nmol Pi/min/mg protein | Human | Substrate: LTC4
pH 7.0, 37°C, recombinant MRP2, using 4mM MgATP and 5g of isolated membranes. |
[1] |
| 125 ± 12 | pmol/min/mg | Human | Substrate LTC4, 37 °C, 4 mM ATP, | [2] |
| 20.2 ± 5.9 | pmol/mg/min | Human | 4 mM ATP, 37°C, 5–10 ml of membrane vesicle suspension (30
mg protein). |
[3] |
References
- ↑ 1.0 1.1 Yasunaga M. "Molecular cloning and functional characterization of cynomolgus monkey multidrug resistance-associated protein 2 (MRP2) Eur. J. Pharm. Sci. 35, 326-334 (2008)
- ↑ 2.0 2.1 Zeng H. "Transport of amphipathic anions by human multidrug resistance protein 3 J. Biol. Chem. 275, 34166-34172 (2000) Cite error: Invalid
<ref>tag; name "Mao2000" defined multiple times with different content - ↑ 3.0 3.1 "Functional reconstitution of substrate transport by purified multidrug resistance protein MRP1 (ABCC1) in phospholipid vesicles Cancer Res. 60, 4779-4784 (2000)
