Difference between revisions of "46BR.1N fibroblasts MTT Assay Results"

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[[Image:Copy_of_Transform_of_Fibroblats_-_use.jpg|none|thumb|500px|The absorbance of various seeding densities (4.4 x10^3 - 9 x10^5 cells/cm2) of 46BR.1N fibroblasts after 48h. The data presented is from three independent experiments. ]]
 
[[Image:Copy_of_Transform_of_Fibroblats_-_use.jpg|none|thumb|500px|The absorbance of various seeding densities (4.4 x10^3 - 9 x10^5 cells/cm2) of 46BR.1N fibroblasts after 48h. The data presented is from three independent experiments. ]]
  
 +
{| class="wikitable"
 +
! style="font-weight:bold;" | Cell number (10^4/cm2)
 +
! style="font-weight:bold;" | A540 nm
 +
|-
 +
| 0
 +
| 0.06 ± 0.01
 +
|-
 +
| 0.44
 +
| 0.10 ± 0.01
 +
|-
 +
| 0.91
 +
| 0.13 ± 0.01
 +
|-
 +
| 4.47
 +
| 0.43 ± 0.06
 +
|-
 +
| 8.94
 +
| 0.46 ± 0.08
 +
|-
 +
| 44.66
 +
| 1.19 ± 0.47
 +
|-
 +
| 89.28
 +
| 1.12 ± 0.21
 +
|}
  
 
== Cell viability ==
 
== Cell viability ==
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[[Image:Copy_of_F+CI_%Viability.jpg|none|thumb|500px|The effect of A23187 (0-5 µM) on the viability of 46BR.1N fibroblasts. Cells were incubated for 6h with the stimulus and the data presented is from six independent experiments. ]]
 
[[Image:Copy_of_F+CI_%Viability.jpg|none|thumb|500px|The effect of A23187 (0-5 µM) on the viability of 46BR.1N fibroblasts. Cells were incubated for 6h with the stimulus and the data presented is from six independent experiments. ]]
  
 +
{| class="wikitable"
 +
! style="font-weight:bold;" | A23187 (uM)
 +
! style="font-weight:bold;" | Cell Viability (% of the control)
 +
|-
 +
| 0
 +
| 100.00 ± 0.00
 +
|-
 +
| 1
 +
| 99.12 ± 19.69
 +
|-
 +
| 3
 +
| 114.22 ± 32.20
 +
|-
 +
| 5
 +
| 110.78 ± 26.82
 +
|-
 +
|}
  
 
[[Image:Copy_of_F+ATP_%Viability.jpg|none|thumb|500px|The effect of ATP (0-8 mM) on the viability of 46BR.1N fibroblasts. Cells were incubated for 6h with the stimulus and the data presented is from six independent experiments. ]]
 
[[Image:Copy_of_F+ATP_%Viability.jpg|none|thumb|500px|The effect of ATP (0-8 mM) on the viability of 46BR.1N fibroblasts. Cells were incubated for 6h with the stimulus and the data presented is from six independent experiments. ]]
  
 +
{| class="wikitable"
 +
! style="font-weight:bold;" | ATP (mM)
 +
! style="font-weight:bold;" | Cell Viability (% of the control)
 +
|-
 +
| 0
 +
| 100.00 ± 0.00
 +
|-
 +
| 0.5
 +
| 89.20 ± 13.15
 +
|-
 +
| 1
 +
| 98.49 ± 8.38
 +
|-
 +
| 2
 +
| 101.10 ± 31.04
 +
|-
 +
| 4
 +
| 86.94 ± 12.78
 +
|-
 +
| 8
 +
| 77.30 ± 18.56
 +
|-
 +
|}
  
 
[[Image:Copy_of_UVR_Firbo_%_viability.jpg|none|thumb|500px|The effect of UVR (0-50 mJ/cm2) on the viability of 46BR.1N fibroblasts. Cells were incubated for 6h following stimulation and the data presented is from three independent experiments.]]
 
[[Image:Copy_of_UVR_Firbo_%_viability.jpg|none|thumb|500px|The effect of UVR (0-50 mJ/cm2) on the viability of 46BR.1N fibroblasts. Cells were incubated for 6h following stimulation and the data presented is from three independent experiments.]]
 +
 +
{| class="wikitable"
 +
! style="font-weight:bold;" | UVR (mJ/cm2)
 +
! style="font-weight:bold;" | Cell Viability (% of the control)
 +
|-
 +
| 0
 +
| 100.00 ± 0.00
 +
|-
 +
| 5
 +
| 123.84 ± 31.35
 +
|-
 +
| 10
 +
| 131.00 ± 13.93
 +
|-
 +
| 15
 +
| 119.26 ± 5.78
 +
|-
 +
| 20
 +
| 108.17 ± 11.40
 +
|-
 +
| 25
 +
| 90.98 ± 5.59
 +
|-
 +
| 50
 +
| 72.98 ± 10.00
 +
|}

Revision as of 07:14, 3 June 2019

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Return to 46BR.1N Fibroblasts

In this study, the aim was to identify optimal conditions for the following experiments. These assays were performed using the MTT assay.

Optimum seeding density

The absorbance of various seeding densities (4.4 x10^3 - 9 x10^5 cells/cm2) of 46BR.1N fibroblasts after 48h. The data presented is from three independent experiments.
Cell number (10^4/cm2) A540 nm
0 0.06 ± 0.01
0.44 0.10 ± 0.01
0.91 0.13 ± 0.01
4.47 0.43 ± 0.06
8.94 0.46 ± 0.08
44.66 1.19 ± 0.47
89.28 1.12 ± 0.21

Cell viability

The effect of A23187 (0-5 µM) on the viability of 46BR.1N fibroblasts. Cells were incubated for 6h with the stimulus and the data presented is from six independent experiments.
A23187 (uM) Cell Viability (% of the control)
0 100.00 ± 0.00
1 99.12 ± 19.69
3 114.22 ± 32.20
5 110.78 ± 26.82
The effect of ATP (0-8 mM) on the viability of 46BR.1N fibroblasts. Cells were incubated for 6h with the stimulus and the data presented is from six independent experiments.
ATP (mM) Cell Viability (% of the control)
0 100.00 ± 0.00
0.5 89.20 ± 13.15
1 98.49 ± 8.38
2 101.10 ± 31.04
4 86.94 ± 12.78
8 77.30 ± 18.56
The effect of UVR (0-50 mJ/cm2) on the viability of 46BR.1N fibroblasts. Cells were incubated for 6h following stimulation and the data presented is from three independent experiments.
UVR (mJ/cm2) Cell Viability (% of the control)
0 100.00 ± 0.00
5 123.84 ± 31.35
10 131.00 ± 13.93
15 119.26 ± 5.78
20 108.17 ± 11.40
25 90.98 ± 5.59
50 72.98 ± 10.00
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