Transformation of TXB2 to exTXB2

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Rate equation

Transport Parameters

K_ms

Literature values

Value	Units	Species	Notes	Weight	Reference
0.0109 ± 0.0041	mM	Human	Substrate: LTC4 pH 7.0, 37°C, recombinant MRP2, using 4mM MgATP and 5g of isolated membranes.	512
0.0000366 ± 0.0000038	mM	Human	Substrate LTC4, 37 °C, 4 mM ATP,	128
5.3E-3 ± 2.6E-3	mM	Human	4 mM ATP, 37°C, 5–10 ml of membrane vesicle suspension (30 mg protein).	128
0.1954 ± 0.0612	mM	Human	pH 7.0, 37°C, recombinant MRP2, Substrate: 17beta-estradiol 17-(beta-D-glucuronide)	512
0.1097 ± 0.0391	mM	Human	pH 7.0, 37°C, recombinant MRP2, Substrate: Estrone glucuronide	512
0.0207	mM	Human	pH 7.4, 37°C, Substrate: Progesterone, Expression vector: Human HepG2 hepatoma	2048

Description of the ABC Kms distribution

Mode (mM)	Confidence Interval	Location parameter (µ)	Scale parameter (σ)
1.13E+01	1.00E+01	3.22E+00	8.91E-01

The estimated probability distribution for ABC Kms. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

k_cat

No data available on the turnover of ABCC4 transporter therefore based upon estimates made by http://book.bionumbers.org/what-are-the-rates-of-membrane-transporters/

Literature values

Value	Units	Transporter	Notes	Weight	Reference
3.5	min-1	Glucose transporter ptsI	Organism: Bacteria Escherichia coli Back of the envelope calculation by BioNumbers: http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=102931	8
0.33 - 0.83	min-1	Rate of transport of H+/Lactose transporter	Organism: Bacteria Escherichia coli Back of the envelop calculation on bionumbers http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=103159	8
3.28	min-1	Catalytic Rate of transporter HXT7	Organism: Budding yeast Saccharomyces cerevisiae Back of the envelope calculation http://bionumbers.hms.harvard.edu/bionumber.aspx?&id=101737	8
11.34	min-1	Human	pH 7.0, 37°C, recombinant MRP2, Substrate: Estrone glucuronide	8

Description of the ABC kcat distribution

Mode (min-1)	Confidence Interval	Location parameter (µ)	Scale parameter (σ)
2.10E-02	2.37E+01	-2.60E+00	1.12E+00

The estimated probability distribution for ABC kcat. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.

V_max

Literature values

Value	Units	Species	Notes	Reference
0.00019 ± 1.96e-5	mmol/min/mg protein	Human	Substrate: LTC4 pH 7.0, 37°C, recombinant MRP2, using 4mM MgATP and 5g of isolated membranes.
1.25e-7 ± 1.2e-8	mmol/min/mg	Human	Substrate LTC4, 37 °C, 4 mM ATP,
2.02e-8 ± 5.9e-9	mmol/mg/min	Human	4 mM ATP, 37°C, 5–10 ml of membrane vesicle suspension (30 mg protein).

Transporter concentration

Literature values

Value	Units	Species	Notes	Weight	Reference
5.15	ppm	Human	Expression Vector: Stomach Enzyme: ABCC4 pH: 7.5 Temperature: 37 °C	2048
5.94	ppm	Human	Expression Vector: Lung Enzyme: ABCC4 pH: 7.5 Temperature: 37 °C	2048
2.66	ppm	Human	Expression Vector: Gut Enzyme: ABCC4 pH: 7.5 Temperature: 37 °C	2048

Description of the ABC concentration distribution

Mode (mM)	Confidence Interval	Location parameter (µ)	Scale parameter (σ)
5.06E+00	1.44E+00	1.74E+00	3.42E-01

The estimated probability distribution for ABC concentration. The value and weight of the literature values used to define the distribution are indicated by an orange dashed line. The x axis is plotted on a log-scale.