Transformation of PGH2 to PGE2
Contents
Reaction
Chemical equation
Rate equation
PGES Enzyme Parameters
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
1.6E-01 ± 4.00E-03 | Human | Expression Vector: E. Coli
Enzyme: PGES pH: 8 Temperature: 37 |
[1] | |
2.15E-01 | Human | Wild Type Enzyme | [2] | |
1.49E-02 | Human | Expression Vector: E. Coli
Enzyme: PGES pH: Unknown Temperature: Unknown Other: cPGES, casein kinase II and Hsp90 |
[3] | |
6.66E-02 | Human | Expression Vector: E. Coli
Enzyme: PGES pH: Unknown Temperature: Unknown |
[3] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
3000 ± 360 | per minute | Human | Expression Vector: E. Coli.
Enzyme: Microsomal Prostaglandin E Synthase pH: 7.5 Temperature: 37 |
[4] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
220 | Human | Expression Vector: Placenta
Enzyme: PGES pH: 7.5 Temperature: 37 °C |
[5] | |
75.3 | Human | Expression Vector: Urinary Bladder
Enzyme: PGES pH: 7.5 Temperature: 37 °C |
[6] | |
208 | Human | Expression Vector: Stomach
Enzyme: PGES pH: 7.5 Temperature: 37 °C |
[5] | |
28.1 | Human | Expression Vector: Lung
Enzyme: PGES pH: 7.5 Temperature: 37 °C |
[6] | |
11.6 | Human | Expression Vector: Colon
Enzyme: PGES pH: 7.5 Temperature: 37 °C |
[6] |
Value | Units | Species | Notes | Reference |
---|---|---|---|---|
5.72 | kcal/mol | Not stated | Estimated
Enzyme: PGES Substrate: Arachidonate Product: PGE2 pH: 7.3 ionic strength: 0.25 |
[7] |
References
- ↑ Pettersson P. , "Identification of beta-trace as prostaglandin D synthase. FASEB J. 2010 Dec;24(12):4668-77. doi: 10.1096/fj.10-164863. Epub 2010 Jul 28.
- ↑ Hamza A. , "Understanding microscopic binding of human microsomal prostaglandin E synthase-1 (mPGES-1) trimer with substrate PGH2 and cofactor GSH: insights from computational alanine scanning and site-directed mutagenesis. J Phys Chem B. 2010 Apr 29;114(16):5605-16. doi: 10.1021/jp100668y.
- ↑ 3.0 3.1 Kobayashi T. , "Regulation of cytosolic prostaglandin E synthase by phosphorylation. Biochem J. 2004 Jul 1;381(Pt 1):59-69.
- ↑ [www.ncbi.nlm.nih.gov/pubmed/16399384 Pettersson P., "Human microsomal prostaglandin E synthase 1: a member of the MAPEG protein superfamily. Methods Enzymol. 2005;401:147-61.]
- ↑ 5.0 5.1 M. Wilhelm Mass-spectrometry-based draft of the human proteome Nature, 2014 509, 582–587
- ↑ 6.0 6.1 6.2 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581
- ↑ Caspi et al 2014, "The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases," Nucleic Acids Research 42:D459-D471