Phosphofructokinase type 1
The enzyme Phosphofructokinase Type-1 uses another ATP molecule to transfer a phosphate group to Fru6P to form fructose 1, 6-bisphosphate. PFK-1 is an allosteric enzyme showing cooperative behaviour with Fru6P and hyperbolic kinetics with ATP.
Chemical equation
![Fru6P + ATP \rightleftharpoons Fru1,6BP + ADP](/wiki/images/math/f/9/3/f931feed721084327fc70fe25f43784b.png)
Rate equation
The concerted transition model of Monod, Wyman and Changeux (MWC model) is used as a rate equation for this tetrameric enzyme for considering exclusive ligand binding (F6P, activators and inhibitors) together with mixed type activation, (Fru2,6BP or AMP or Pi).
![\left (\left (\frac{\frac{[ATP]}{K_{ATP}}}{1 + \frac{[ATP]}{K_{ATP}} }\right ) \left ( \frac{ 1 + \frac{[\beta Fru2,6BP]}{ \alpha Ka_{Fru2,6BP} } }{ 1 + \frac{[Fru2,6BP]}{ \alpha Ka_{Fru2,6BP} } } \right )
\left( \frac{\frac{[Fru6P]\left(1+\frac{[Fru2,6BP]}{[\alpha Ka_{Fru2,6BP}]}\right)}{K_{Fru6P}\left(1 + \frac{[Fru2,6BP]}{Ka_{Fru2,6BP}}\right)} \left[1 + \frac{[Fru6P]\left(1+\frac{[Fru2,6BP]}{\alpha Ka_{Fru2,6BP}}\right)}{K_{Fru6P\left(1 + \frac{[Fru2n6BP]}{Ka_{Fru2,6BP}}\right)}} \right]^3}
{ \frac{L\left( 1 + \frac{[CIT]}{Ki_{CIT}}\right)^4\left(1 + \frac{[ATP]}{Ki_{ATP}}\right)^4}{\left(1+\frac{[Fru2,6BP]}{Ka_{Fru2,6BP}}\right)^4} + \left[1 + \frac{Fru6P\left(1+\frac{Fru2,6BP}{\alpha Ka_{Fru2,6BP}}\right)}{K_{Fru6P\left(1 + \frac{[Fru2n6BP]}{Ka_{Fru2,6BP}}\right)}} \right]^4 } \right) - \left( \frac{\frac{[ADP][F1,6BP]}{K_{ADP}K_{F1,6BP}K_{eq}}}{\frac{[ADP]}{K_{ADP}} + \frac{[Fru1,6BP]}{K_{Fru1,6BP}} + \frac{[ADP][Fru1,6BP]}{K_{ADP}K_{Fru1,6BP}} + 1 } \right) \right)](/wiki/images/math/4/2/7/427343d27db0b7ccd516330c30e4f5c6.png)
Parameter values
Parameter | Value | Organism | Remarks |
---|---|---|---|
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0.06 [1] | HeLa cell line | |
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651[2] | ||
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0.1[3] | ||
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1.1[3] | ||
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3.5[3] |
Alternative parameter values
- ↑ Marín-Hernández A , Rodríguez-Enríquez S, Vital-González P A, et al. (2006). Determining and understanding the control of glycolysis in fast-growth tumor cells. Flux control by an over-expressed but strongly product-inhibited hexokinase. FEBS J., 273 , pp. 1975–1988(doi)
- ↑ Arbitrary value
- ↑ 3.0 3.1 3.2 3.3 Cite error: Invalid
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tag; no text was provided for refs namedHernandez2011