Transformation of LTA4 to LTC4

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Reaction

Chemical equation

 AA \rightleftharpoons PGH2

Rate equation

Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
0.3 �± 0.06 mM Human Wild Type hLTC4S, 20 °C, pH 7.8, incubated for 15 seconds. [1]
3.00E-02 ± 1.00E-02 mM Human Wild type, pH 7.8 [2]
Enzyme Turnover Numbers
Value Units Species Notes Reference
702 per minute Human Wild Type hLTC4S, 20 °C, pH 7.8, incubated for 15 seconds. [1]
1560 ± 240 per minute Human Wild Type, pH 7.8 [2]
Enzyme Turnover Numbers
Value Units Species Notes Reference
1.21E-04 mM Human Calculated from 20ug of 16.5kDa protein.

Wild Type hLTC4S, 20 °C, pH 7.8, incubated for 15 seconds.

[1]
6.06e-05 - 3.03e-05 mM Human Wild Type, pH 7.8 [2]

References

  1. 1.0 1.1 1.2 [http://www.jbc.org/content/285/52/40771.full.pdf Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4 Synthase J Biochem 2010, 285, 40771-40776] Cite error: Invalid <ref> tag; name "Rinaldo2010" defined multiple times with different content
  2. 2.0 2.1 2.2 [http://www.ncbi.nlm.nih.gov/pubmed/19010934 Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the enzyme mechanism J. Biol. Chem. 289, 5199-5207 (2014)] Cite error: Invalid <ref> tag; name "Niegowski2013" defined multiple times with different content Cite error: Invalid <ref> tag; name "Niegowski2013" defined multiple times with different content

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