Antisense interaction between r and a
The full length mRNA of scbR (r) binds to the full length mRNA of scbA (a) and form a complex which prevents further translation of both mRNAs.
Contents
Chemical equation
Rate equation
Parameters
The parameter of this reaction is the dissociation constant for binding of scbR mRNA to scbA mRNA (Failed to parse (Cannot store math image on filesystem.): K_{ra} ).
Name | Value | Units | Origin | Remarks |
---|---|---|---|---|
Failed to parse (Cannot store math image on filesystem.): 0.06-0.18 [1] [2] [3] | Failed to parse (Cannot store math image on filesystem.): nM^{-1} \cdot min^{-1} | measurements of antisense mRNA interactions | ||
Failed to parse (Cannot store math image on filesystem.): K_{-ar} | Failed to parse (Cannot store math image on filesystem.): 0.006-6 |
Parameters with uncertainty
The most plausible parameter value for the is decided to be and the confidence interval . This means that the mode of the PDF is 5 and the range where 95% of the values are found is between ?? and ??.
Similarly, the most plausible parameter value for the Failed to parse (Cannot store math image on filesystem.): K_{-ar} is decided to be Failed to parse (Cannot store math image on filesystem.): 0.6 and the confidence interval Failed to parse (Cannot store math image on filesystem.): 0.1 . This means that the mode of the PDF is 0.6 and the range where 95% of the values are found is between 0.06 and 6.
The probability distributions for the two parameters, adjusted accordingly in order to reflect the above values, are the following:
The location and scale parameters of the distribution are:
Parameter | μ | σ |
---|---|---|
Failed to parse (Cannot store math image on filesystem.): K_{-ar} |
References
- ↑ Shokeen S, Johnson CM, Greenfield TJ, Manias DA, Dunny GM, Weaver KE. Structural analysis of the Anti-Q-Qs interaction: RNA-mediated regulation of E. faecalis plasmid pCF10 conjugation. Plasmid. 2010;64(1):26-35.
- ↑ Brantl S. Regulatory mechanisms employed by cis-encoded antisense RNAs. Cell regulation (RNA special issue). 2007; 10: 102–109.
- ↑ Eguchi Y, Itoh T, Tomizawa J (1991) Antisense Rna. Annual Review of Biochemistry 60: 631–652.