Phosphofructokinase type 1

The enzyme Phosphofructokinase Type-1 uses another ATP molecule to transfer a phosphate group to fructose 6-phosphate to form fructose 1, 6-bisphosphate. PFK-1 is an allosteric enzyme showing cooperative behaviour with Fru6P and hyperbolic kinetics with ATP.

Chemical equation

$Fru6P + ATP \rightleftharpoons Fru1,6BP + ADP$

Rate equation

The concerted transition model of Monod, Wyman and Changeux (MWC model) is used as a rate equation for this tetrameric enzyme for considering exclusive ligand binding (F6P, activators and inhibitors) together with mixed type activation, (Fru2,6BP or AMP or Pi).

$\left (\left (\frac{\frac{[ATP]}{K_{ATP}}}{1 + \frac{[ATP]}{K_{ATP}} }\right ) \left ( \frac{ 1 + \frac{\beta Fru2,6BP}{ \alpha Ka_{Fru2,6BP} } }{ 1 + \frac{Fru2,6BP}{ \alpha Ka_{Fru2,6BP} } } \right ) \left( \frac{\frac{Fru6P\left(1+\frac{Fru2,6BP}{\alpha Ka_{Fru2,6BP}}\right)}{K_{Fru6P\left(1 + \frac{Fru2n6BP}{Ka_{Fru2,6BP}}\right)}} \left[1 + \frac{Fru6P\left(1+\frac{Fru2,6BP}{\alpha Ka_{Fru2,6BP}}\right)}{K_{Fru6P\left(1 + \frac{Fru2n6BP}{Ka_{Fru2,6BP}}\right)}} \right]^3} { \frac{L( 1 + \frac{CIT}{Ki_{CIT}})^4(1 + \frac{ATP}{Ki_{ATP}})^4}{(1+\frac{Fru2,6BP}{Ka_{Fru2,6BP}})^4} + \left[1 + \frac{Fru6P\left(1+\frac{Fru2,6BP}{\alpha Ka_{Fru2,6BP}}\right)}{K_{Fru6P\left(1 + \frac{Fru2n6BP}{Ka_{Fru2,6BP}}\right)}} \right]^4 } \right)\right)$