Phosphofructokinase type 1

The enzyme Phosphofructokinase Type-1 uses another ATP molecule to transfer a phosphate group to fructose 6-phosphate to form fructose 1, 6-bisphosphate. PFK-1 is an allosteric enzyme showing cooperative behaviour with Fru6P and hyperbolic kinetics with ATP.

Chemical equation

$Fru6P + ATP \rightleftharpoons Fru1,6BP + ADP$

Rate equation

The concerted transition model of Monod, Wyman and Changeux (MWC model) is used as a rate equation for this tetrameric enzyme for considering exclusive ligand binding (F6P, activators and inhibitors) together with mixed type activation, (Fru2,6BP or AMP or Pi).

$\left(\left(\frac{\frac{[ATP]}{K_{ATP}}}{1 + \frac{[ATP]}{K_{ATP}} }\right) \left( \frac{ 1 + \frac{\beta Fru2,6BP}{ \alpha Ka_{Fru2,6BP} } }{ 1 + \frac{Fru2,6BP}{ \alpha Ka_{Fru2,6BP} } } \right) \right)$