Phosphofructokinase type 1
The enzyme Phosphofructokinase Type-1 uses another ATP molecule to transfer a phosphate group to Fru6P to form fructose 1, 6-bisphosphate. PFK-1 is an allosteric enzyme showing cooperative behaviour with Fru6P and hyperbolic kinetics with ATP.
Contents
Chemical equation
Rate equation
The concerted transition model of Monod, Wyman and Changeux (MWC model) is used as a rate equation for this tetrameric enzyme for considering exclusive ligand binding (F6P, activators and inhibitors) together with mixed type activation, (Fru2,6BP or AMP or Pi) [1].
Parameter values
Parameter | Value | Units | Organism | Remarks |
---|---|---|---|---|
0.031 [1] | HeLa cell line | Moreno-Sánchez, Marín-Hernández, Encalada & Saavedra, unpublished results | ||
1.0 [1] | mM | |||
0.021[1] | mM | |||
20[1] | mM | |||
6.8[1] | mM | |||
[1] | mM | |||
0.32[1] | Dimensionless | |||
0.98[1] | Dimensionless | |||
4.1[1] | Dimensionless | |||
5[1] | mM | |||
5[1] | mM | |||
247[1] | mM | Recalculated from the |
Parameters with uncertainty
- The and in the rate equation represents the factors by which the ligand affinity and catalytic capacity are modified in the presence of an allosteric activatory [2]. As is the only activator in our model we considered the and value of , and . These two values are measured in the presence of 140 Failed to parse (lexing error): \text{mM K^{+}} .
References
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Marín-Hernández A, Gallardo-Pérez JC, Rodríguez-Enríquez S et al (2011). Modeling cancer glycolysis. Biochim Biophys Acta, 1807:755–767 (doi)
- ↑ R. Moreno-Sánchez, A. Marín-Hernández, J.C. Gallardo-Pérez, H. Quezada, R. Encalada, S. Rodríguez-Enríquez et al. (2012), Phosphofructokinase type 1 kinetics, isoform expression, and gene polymorphisms in cancer cells, J Cell Biochem, 113, pp. 1692–1703