The enzyme Phosphofructokinase Type-1 uses another ATP molecule to transfer a phosphate group to Fru6P to form fructose 1, 6-bisphosphate. PFK-1 is an allosteric enzyme showing cooperative behaviour with Fru6P and hyperbolic kinetics with ATP.
Chemical equation
Rate equation
The concerted transition model of Monod, Wyman and Changeux (MWC model) is used as a rate equation for this tetrameric enzyme for considering exclusive ligand binding (F6P, activators and inhibitors) together with mixed type activation, (Fru2,6BP or AMP or Pi) [1].
Failed to parse (Cannot store math image on filesystem.): v = Vm\left (\left (\frac{\frac{[ATP]}{Km_{ATP}}}{1 + \frac{[ATP]}{Km_{ATP}} }\right ) \left ( \frac{ 1 + \frac{[\beta Fru2,6BP]}{ \alpha Ka_{Fru2,6BP} } }{ 1 + \frac{[Fru2,6BP]}{ \alpha Ka_{Fru2,6BP} } } \right ) \left( \frac{\frac{[Fru6P]\left(1+\frac{[Fru2,6BP]}{[\alpha Ka_{Fru2,6BP}]}\right)}{Km_{Fru6P}\left(1 + \frac{[Fru2,6BP]}{Ka_{Fru2,6BP}}\right)} \left[1 + \frac{[Fru6P]\left(1+\frac{[Fru2,6BP]}{\alpha Ka_{Fru2,6BP}}\right)}{Km_{Fru6P}\left(1 + \frac{[Fru2,6BP]}{Ka_{Fru2,6BP}}\right)} \right]^3} { \frac{L\left( 1 + \frac{[CIT]}{Ki_{CIT}}\right)^4\left(1 + \frac{[ATP]}{Ki_{ATP}}\right)^4}{\left(1+\frac{[Fru2,6BP]}{Ka_{Fru2,6BP}}\right)^4} + \left[1 + \frac{Fru6P\left(1+\frac{Fru2,6BP}{\alpha Ka_{Fru2,6BP}}\right)}{Km_{Fru6P}\left(1 + \frac{[Fru2,6BP]}{Ka_{Fru2,6BP}}\right)} \right]^4 } - \left( \frac{\frac{[ADP][Fru1,6BP]}{K_{ADP}K_{Fru1,6BP}K_{eq}}}{\frac{[ADP]}{K_{ADP}} + \frac{[Fru1,6BP]}{K_{Fru1,6BP}} + \frac{[ADP][Fru1,6BP]}{K_{ADP}K_{Fru1,6BP}} + 1 } \right) \right) \right)
Parameter values
Parameter
|
Value
|
Units
|
Organism
|
Remarks
|
|
0.031 [1]
|
|
HeLa cell line
|
Moreno-Sánchez, Marín-Hernández, Encalada & Saavedra, unpublished results
|
|
1.0 [1]
|
mM
|
|
0.021[1]
|
mM
|
|
20[1]
|
mM
|
|
6.8[1]
|
mM
|
|
[1]
|
mM
|
|
0.32[1]
|
Dimensionless
|
|
0.98[1]
|
Dimensionless
|
|
4.1[1]
|
Dimensionless
|
|
5[1]
|
mM
|
|
5[1]
|
mM
|
|
247[1]
|
mM
|
|
Recalculated from the
|
Alternative parameter values
References