Difference between revisions of "Transformation of LTA4 to LTC4"

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(→‎Parameters)
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{|class="wikitable sortable"  
 
{|class="wikitable sortable"  
−
|+  style="text-align: left;" | Enzyme Turnover Numbers
+
|+  style="text-align: left;" | LTC4S Abundance
 
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|-
 
! Value
 
! Value
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! Reference
 
! Reference
 
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|-
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|1.21E-04
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|26.8
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|mM
+
|<math> ppm </math>
−
|Human
+
|Human  
−
|Expression Vector: E. Coli.
+
|Expression Vector: Lung
−
Enzyme: Wild Type hLTC4S
+
Enzyme: LTC4S
−
pH:7.8
+
pH: 7.5
−
Temperature:20 °C
+
Temperature: 37 °C
−
Calculated from 20ug of 16.5kDa protein.
+
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
−
|<ref name="Rinaldo2010"> [http://www.jbc.org/content/285/52/40771.full.pdf Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4
+
|-
−
Synthase'' J Biochem 2010, 285, 40771-40776]</ref>
+
|33.0
 +
|<math> ppm </math>
 +
|Human
 +
|Expression Vector: Esophagus
 +
Enzyme: LTC4S
 +
pH: 7.5
 +
Temperature: 37 °C
 +
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
 
|-
 
|-
−
|6.06e-05 - 3.03e-05
+
|13.9
−
|mM
+
|<math> ppm </math>
−
|Human
+
|Human  
−
|Expression Vector: E Coli
+
|Expression Vector: Adrenal Gland
−
Enzyme: Wild type LTC4S
+
Enzyme: LTC4S
−
pH: 7.8
+
pH: 7.5
 
Temperature: 37 °C
 
Temperature: 37 °C
−
|<ref name="Niegowski2013"> [http://www.jbc.org/content/289/8/5199.full Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the
+
|<ref name="Kim2014"> [http://www.nature.com/nature/journal/v509/n7502/pdf/nature13302.pdf M. Kim ''A draft map of the human proteome'' Nature, 2014 509, 575–581]</ref>
−
enzyme mechanism'' J. Biol. Chem. 289, 5199-5207 (2014)]</ref>
 
 
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|-
 
|}
 
|}

Revision as of 12:02, 12 August 2016

Return to overview

Reaction

Chemical equation

AA \rightleftharpoons PGH2

Rate equation

Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
0.3 �± 0.06 mM Human Expression Vector: E. Coli.

Enzyme: Wild Type hLTC4S pH:7.8 Temperature:20 °C

[1]
3.00E-02 ± 1.00E-02 mM Human Expression Vector: E Coli

Enzyme: Wild type LTC4S pH: 7.8 Temperature: 37 °C

[2]
Enzyme Turnover Numbers
Value Units Species Notes Reference
702 per minute Human Expression Vector: E. Coli.

Enzyme: Wild Type hLTC4S pH:7.8 Temperature:20 °C

[1]
1560 ± 240 per minute Human Expression Vector: E Coli

Enzyme: Wild type LTC4S pH: 7.8 Temperature: 37 °C

[2]
LTC4S Abundance
Value Units Species Notes Reference
26.8 ppm Human Expression Vector: Lung

Enzyme: LTC4S pH: 7.5 Temperature: 37 °C

[3]
33.0 ppm Human Expression Vector: Esophagus

Enzyme: LTC4S pH: 7.5 Temperature: 37 °C

[3]
13.9 ppm Human Expression Vector: Adrenal Gland

Enzyme: LTC4S pH: 7.5 Temperature: 37 °C

[3]

References

  1. ↑ 1.0 1.1 [http://www.jbc.org/content/285/52/40771.full.pdf Rinaldo A. " Arginine 104 Is a Key Catalytic Residue in Leukotriene C4 Synthase J Biochem 2010, 285, 40771-40776]
  2. ↑ 2.0 2.1 [http://www.jbc.org/content/early/2013/12/23/jbc.M113.534628 Niegowski D. " Crystal structures of Leukotriene C4 synthase in complex with product analogs, implications for the enzyme mechanism J. Biol. Chem. 289, 5199-5207 (2014)] Cite error: Invalid <ref> tag; name "Niegowski2013" defined multiple times with different content
  3. ↑ 3.0 3.1 3.2 M. Kim A draft map of the human proteome Nature, 2014 509, 575–581

Related Reactions

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