Difference between revisions of "Transformation of PGH2 to PGE2"

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(PGES Enzyme Parameters)
(PGES Enzyme Parameters)
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| per minute
 
| per minute
 
| Human
 
| Human
| Microsomal Prostaglandin E Synthase 1 expressed in E. Coli.
+
||Expression Vector: E. Coli.
 +
Enzyme: Microsomal Prostaglandin E Synthase
 +
pH: 7.5
 +
Temperature: 37
 +
 
 
| <ref name="Pettersson20005"> [www.ncbi.nlm.nih.gov/pubmed/16399384 Pettersson P., "Human microsomal prostaglandin E synthase 1: a member of the MAPEG protein superfamily.'' Methods Enzymol. 2005;401:147-61.]</ref>  
 
| <ref name="Pettersson20005"> [www.ncbi.nlm.nih.gov/pubmed/16399384 Pettersson P., "Human microsomal prostaglandin E synthase 1: a member of the MAPEG protein superfamily.'' Methods Enzymol. 2005;401:147-61.]</ref>  
 
|-
 
|-

Revision as of 09:48, 2 June 2016

Return to overview

Reaction

R10 PGH2 - PGE2.jpg

Chemical equation

 FA \rightleftharpoons AA

Rate equation

PGES Enzyme Parameters

Michaelis-Menten Constants
Value Units Species Notes Reference
1.6E-01 ± 4.00E-03  mM Human Expression Vector: E. Coli

Enzyme: PGES pH: 8 Temperature: 37

[1]
2.15E-01  mM Human Wild Type Enzyme [2]
1.49E-02  mM Human Expression Vector: E. Coli

Enzyme: PGES pH: Unknown Temperature: Unknown

Other: cPGES, casein kinase II and Hsp90

[3]
6.66E-02  mM Human Expression Vector: E. Coli

Enzyme: PGES pH: Unknown Temperature: Unknown

[3]
Enzyme Turnover Numbers
Value Units Species Notes Reference
3000 ± 360 per minute Human Expression Vector: E. Coli.

Enzyme: Microsomal Prostaglandin E Synthase pH: 7.5 Temperature: 37

[4]
Enzyme Concentrations
Value Units Species Notes Reference
0.00002353 mM Human 40ng/100ul of a 17 kDa protein

Synovial Tissue

[1]
0.47059 mM Human 8 mg/mL of 17kDa of protien [2]

References


Related Reactions